Biotransformations of nitriles mediated by in vivo nitrile hydratase of Rhodococcus erythropolis ATCC 4277 heterologously expressed in E. coli
Nitrile hydratase activity has been reported as an exciting alternative for the industrial production of a variety of compounds overwhelming its chemical counterpart; despite this, until now, a few enzymes have been thoroughly studied. Efficient expression of nitrile hydratase enzymes has been the b...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715622004799 |
| _version_ | 1797799115899273216 |
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| author | Maraylla I. Moraes César Iglesias Iris S. Teixeira Humberto M.S. Milagre Sonia Rodríguez Giordano Cintia D.F. Milagre |
| author_facet | Maraylla I. Moraes César Iglesias Iris S. Teixeira Humberto M.S. Milagre Sonia Rodríguez Giordano Cintia D.F. Milagre |
| author_sort | Maraylla I. Moraes |
| collection | DOAJ |
| description | Nitrile hydratase activity has been reported as an exciting alternative for the industrial production of a variety of compounds overwhelming its chemical counterpart; despite this, until now, a few enzymes have been thoroughly studied. Efficient expression of nitrile hydratase enzymes has been the bottleneck to explore this activity. Here, we report the cloning and expression of Rhodococcus erythropolis ATCC 4277 nitrile hydratase (α- and β-subunits) and the correspondent activator gene. Furthermore, substrate scope with whole cells of recombinant E. coli demonstrates that this Fe-type NHase could hydrate a wide range of aliphatic and aromatic nitrile with high conversion rates and moderate enantiomeric excess. |
| first_indexed | 2024-03-13T04:14:07Z |
| format | Article |
| id | doaj.art-f636f09fdd404b4caedae69daea96d24 |
| institution | Directory Open Access Journal |
| issn | 2211-7156 |
| language | English |
| last_indexed | 2024-03-13T04:14:07Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj.art-f636f09fdd404b4caedae69daea96d242023-06-21T06:51:52ZengElsevierResults in Chemistry2211-71562023-01-015100760Biotransformations of nitriles mediated by in vivo nitrile hydratase of Rhodococcus erythropolis ATCC 4277 heterologously expressed in E. coliMaraylla I. Moraes0César Iglesias1Iris S. Teixeira2Humberto M.S. Milagre3Sonia Rodríguez Giordano4Cintia D.F. Milagre5Institute of Chemistry, UNESP – São Paulo State University, Araraquara, São Paulo, BrazilArea Microbiología, DEPBIO, Facultad de Química, Universidad de la República, Gral. Flores 2124, Montevideo 11800, UruguayInstitute of Chemistry, UNESP – São Paulo State University, Araraquara, São Paulo, BrazilInstitute of Chemistry, UNESP – São Paulo State University, Araraquara, São Paulo, BrazilArea Microbiología, DEPBIO, Facultad de Química, Universidad de la República, Gral. Flores 2124, Montevideo 11800, Uruguay; Corresponding authors.Institute of Chemistry, UNESP – São Paulo State University, Araraquara, São Paulo, Brazil; Corresponding authors.Nitrile hydratase activity has been reported as an exciting alternative for the industrial production of a variety of compounds overwhelming its chemical counterpart; despite this, until now, a few enzymes have been thoroughly studied. Efficient expression of nitrile hydratase enzymes has been the bottleneck to explore this activity. Here, we report the cloning and expression of Rhodococcus erythropolis ATCC 4277 nitrile hydratase (α- and β-subunits) and the correspondent activator gene. Furthermore, substrate scope with whole cells of recombinant E. coli demonstrates that this Fe-type NHase could hydrate a wide range of aliphatic and aromatic nitrile with high conversion rates and moderate enantiomeric excess.http://www.sciencedirect.com/science/article/pii/S2211715622004799BiocatalysisNitrile hydrataseRestriction free cloningNitrilesAmides |
| spellingShingle | Maraylla I. Moraes César Iglesias Iris S. Teixeira Humberto M.S. Milagre Sonia Rodríguez Giordano Cintia D.F. Milagre Biotransformations of nitriles mediated by in vivo nitrile hydratase of Rhodococcus erythropolis ATCC 4277 heterologously expressed in E. coli Results in Chemistry Biocatalysis Nitrile hydratase Restriction free cloning Nitriles Amides |
| title | Biotransformations of nitriles mediated by in vivo nitrile hydratase of Rhodococcus erythropolis ATCC 4277 heterologously expressed in E. coli |
| title_full | Biotransformations of nitriles mediated by in vivo nitrile hydratase of Rhodococcus erythropolis ATCC 4277 heterologously expressed in E. coli |
| title_fullStr | Biotransformations of nitriles mediated by in vivo nitrile hydratase of Rhodococcus erythropolis ATCC 4277 heterologously expressed in E. coli |
| title_full_unstemmed | Biotransformations of nitriles mediated by in vivo nitrile hydratase of Rhodococcus erythropolis ATCC 4277 heterologously expressed in E. coli |
| title_short | Biotransformations of nitriles mediated by in vivo nitrile hydratase of Rhodococcus erythropolis ATCC 4277 heterologously expressed in E. coli |
| title_sort | biotransformations of nitriles mediated by in vivo nitrile hydratase of rhodococcus erythropolis atcc 4277 heterologously expressed in e coli |
| topic | Biocatalysis Nitrile hydratase Restriction free cloning Nitriles Amides |
| url | http://www.sciencedirect.com/science/article/pii/S2211715622004799 |
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