Significant Effect of Crocin on the Gene Expression of MicroRNA-21 and MicroRNA-155 in Patients with Osteoarthritis
Osteoarthritis (OA) is the most common form of arthritis associated with gradual joint destruction. The current treatment aims to alleviate pain and inflammation and improve the quality of life. Crocin is an active ingredient in saffron, with anti-inflammatory properties. MicroRNAs are small, non-c...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2022-06-01
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| Series: | Iranian Journal of Allergy, Asthma and Immunology |
| Subjects: | |
| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/3441 |
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| author | Maryam Mohebbi Mahdi Atabaki Jalil Tavakkol-Afshari Zhaleh Shariati-Sarabi Javad Poursamimi Seyed Ahmad Mohajeri Mojgan Mohammadi |
| author_facet | Maryam Mohebbi Mahdi Atabaki Jalil Tavakkol-Afshari Zhaleh Shariati-Sarabi Javad Poursamimi Seyed Ahmad Mohajeri Mojgan Mohammadi |
| author_sort | Maryam Mohebbi |
| collection | DOAJ |
| description |
Osteoarthritis (OA) is the most common form of arthritis associated with gradual joint destruction. The current treatment aims to alleviate pain and inflammation and improve the quality of life. Crocin is an active ingredient in saffron, with anti-inflammatory properties. MicroRNAs are small, non-coding RNAs that regulate gene expression. We aimed to evaluate the effect of crocin on the gene expression of microRNA-146a, microRNA-155, microRNA-223, and microRNA-21 in OA patients and compare it with a placebo.
This study was approved and registered in the Iranian Registry of Clinical Trials (2015021910507N2) and ClinicalTrials.gov identifier: NCT03375814. Forty OA patients were randomly divided into two equal groups, receiving either crocin or placebo. Peripheral blood samples were collected before and four months after the intervention. The pain was assessed using the visual analog scale, and laboratory tests included C-reactive protein and erythrocyte sedimentation rate. The expression levels of microRNA-146a, microRNA-155, microRNA-223, and microRNA-21 genes were evaluated by SYBR Green real-time PCR.
The results showed that the gene expression levels of microRNA-21 and microRNA-155 in patients receiving crocin were significantly decreased and increased, respectively. No significant changes were observed in microRNA-146a and microRNA-223 gene expression levels.
In conclusion, crocin's anti-inflammatory role might be partly attributed to its effects on the gene expression of microRNA-21 and microRNA-155.
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| first_indexed | 2024-12-10T23:43:48Z |
| format | Article |
| id | doaj.art-f63761f4123448cea64c2105d2679b6e |
| institution | Directory Open Access Journal |
| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-12-10T23:43:48Z |
| publishDate | 2022-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-f63761f4123448cea64c2105d2679b6e2022-12-22T01:28:58ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492022-06-0121310.18502/ijaai.v21i3.9805Significant Effect of Crocin on the Gene Expression of MicroRNA-21 and MicroRNA-155 in Patients with OsteoarthritisMaryam Mohebbi0Mahdi Atabaki1Jalil Tavakkol-Afshari2Zhaleh Shariati-Sarabi3Javad Poursamimi4Seyed Ahmad Mohajeri5Mojgan Mohammadi6Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, IranClinical Immunology Research Center, Zahedan University of Medical Sciences, Zahedan, IranImmunology Research Center, Mashhad University of Medical Sciences, Mashhad, IranRheumatic Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Immunology, School of Medicine, Zabol University of Medical Sciences, Zabol, IranPharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranImmunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Osteoarthritis (OA) is the most common form of arthritis associated with gradual joint destruction. The current treatment aims to alleviate pain and inflammation and improve the quality of life. Crocin is an active ingredient in saffron, with anti-inflammatory properties. MicroRNAs are small, non-coding RNAs that regulate gene expression. We aimed to evaluate the effect of crocin on the gene expression of microRNA-146a, microRNA-155, microRNA-223, and microRNA-21 in OA patients and compare it with a placebo. This study was approved and registered in the Iranian Registry of Clinical Trials (2015021910507N2) and ClinicalTrials.gov identifier: NCT03375814. Forty OA patients were randomly divided into two equal groups, receiving either crocin or placebo. Peripheral blood samples were collected before and four months after the intervention. The pain was assessed using the visual analog scale, and laboratory tests included C-reactive protein and erythrocyte sedimentation rate. The expression levels of microRNA-146a, microRNA-155, microRNA-223, and microRNA-21 genes were evaluated by SYBR Green real-time PCR. The results showed that the gene expression levels of microRNA-21 and microRNA-155 in patients receiving crocin were significantly decreased and increased, respectively. No significant changes were observed in microRNA-146a and microRNA-223 gene expression levels. In conclusion, crocin's anti-inflammatory role might be partly attributed to its effects on the gene expression of microRNA-21 and microRNA-155. https://ijaai.tums.ac.ir/index.php/ijaai/article/view/3441Clinical trialInflammationMicroRNAsOsteoarthritis |
| spellingShingle | Maryam Mohebbi Mahdi Atabaki Jalil Tavakkol-Afshari Zhaleh Shariati-Sarabi Javad Poursamimi Seyed Ahmad Mohajeri Mojgan Mohammadi Significant Effect of Crocin on the Gene Expression of MicroRNA-21 and MicroRNA-155 in Patients with Osteoarthritis Iranian Journal of Allergy, Asthma and Immunology Clinical trial Inflammation MicroRNAs Osteoarthritis |
| title | Significant Effect of Crocin on the Gene Expression of MicroRNA-21 and MicroRNA-155 in Patients with Osteoarthritis |
| title_full | Significant Effect of Crocin on the Gene Expression of MicroRNA-21 and MicroRNA-155 in Patients with Osteoarthritis |
| title_fullStr | Significant Effect of Crocin on the Gene Expression of MicroRNA-21 and MicroRNA-155 in Patients with Osteoarthritis |
| title_full_unstemmed | Significant Effect of Crocin on the Gene Expression of MicroRNA-21 and MicroRNA-155 in Patients with Osteoarthritis |
| title_short | Significant Effect of Crocin on the Gene Expression of MicroRNA-21 and MicroRNA-155 in Patients with Osteoarthritis |
| title_sort | significant effect of crocin on the gene expression of microrna 21 and microrna 155 in patients with osteoarthritis |
| topic | Clinical trial Inflammation MicroRNAs Osteoarthritis |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/3441 |
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