Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position
Accurate translation of the genetic code depends on mRNA:tRNA codon:anticodon base pairing. Here we exploit an emissive, isosteric adenosine surrogate that allows direct measurement of the kinetics of codon:anticodon University of California base formation during protein synthesis. Our results sugge...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-08-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/22/9/1427 |
| _version_ | 1828825744038428672 |
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| author | Wei Liu Dongwon Shin Martin Ng Karissa Y. Sanbonmatsu Yitzhak Tor Barry S. Cooperman |
| author_facet | Wei Liu Dongwon Shin Martin Ng Karissa Y. Sanbonmatsu Yitzhak Tor Barry S. Cooperman |
| author_sort | Wei Liu |
| collection | DOAJ |
| description | Accurate translation of the genetic code depends on mRNA:tRNA codon:anticodon base pairing. Here we exploit an emissive, isosteric adenosine surrogate that allows direct measurement of the kinetics of codon:anticodon University of California base formation during protein synthesis. Our results suggest that codon:anticodon base pairing is subject to tighter constraints at the middle position than at the 5′- and 3′-positions, and further suggest a sequential mechanism of formation of the three base pairs in the codon:anticodon helix. |
| first_indexed | 2024-12-12T14:24:44Z |
| format | Article |
| id | doaj.art-f63a13cc2c9f444586a88a84d0382c06 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T14:24:44Z |
| publishDate | 2017-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f63a13cc2c9f444586a88a84d0382c062022-12-22T00:21:43ZengMDPI AGMolecules1420-30492017-08-01229142710.3390/molecules22091427molecules22091427Stringent Nucleotide Recognition by the Ribosome at the Middle Codon PositionWei Liu0Dongwon Shin1Martin Ng2Karissa Y. Sanbonmatsu3Yitzhak Tor4Barry S. Cooperman5Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USADepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USADepartment of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USATheoretical Biology and Biophysics Group, Los Alamos National Laboratory, Los Alamos, NM 87545, USADepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USADepartment of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USAAccurate translation of the genetic code depends on mRNA:tRNA codon:anticodon base pairing. Here we exploit an emissive, isosteric adenosine surrogate that allows direct measurement of the kinetics of codon:anticodon University of California base formation during protein synthesis. Our results suggest that codon:anticodon base pairing is subject to tighter constraints at the middle position than at the 5′- and 3′-positions, and further suggest a sequential mechanism of formation of the three base pairs in the codon:anticodon helix.https://www.mdpi.com/1420-3049/22/9/1427fluorescent mRNAmRNA:tRNA base pairingkinetic mechanismsteric constraintatomic mutagenesis |
| spellingShingle | Wei Liu Dongwon Shin Martin Ng Karissa Y. Sanbonmatsu Yitzhak Tor Barry S. Cooperman Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position Molecules fluorescent mRNA mRNA:tRNA base pairing kinetic mechanism steric constraint atomic mutagenesis |
| title | Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position |
| title_full | Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position |
| title_fullStr | Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position |
| title_full_unstemmed | Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position |
| title_short | Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position |
| title_sort | stringent nucleotide recognition by the ribosome at the middle codon position |
| topic | fluorescent mRNA mRNA:tRNA base pairing kinetic mechanism steric constraint atomic mutagenesis |
| url | https://www.mdpi.com/1420-3049/22/9/1427 |
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