Circulating MiRNA-195-5p and -451a in Diabetic Patients with Transient and Acute Ischemic Stroke in the Emergency Department

(1) Background: Circulating micro-RNAs (miRNAs) modulate the expression of molecules in diabetes. We evaluated the expression of serum miRNA-195-5p and -451a in diabetic patients with ischemic stroke and correlated them with two markers of brain tissue integrity. (2) Methods: Seventy-eight subjects...

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Main Authors: Mauro Giordano, Maria Consiglia Trotta, Tiziana Ciarambino, Michele D’Amico, Marilena Galdiero, Federico Schettini, Diego Paternosto, Marta Salzillo, Roberto Alfano, Vincenzo Andreone, Lorenzo Salvatore Malatino, Gianni Biolo, Giuseppe Paolisso, Luigi Elio Adinolfi
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/21/20/7615
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author Mauro Giordano
Maria Consiglia Trotta
Tiziana Ciarambino
Michele D’Amico
Marilena Galdiero
Federico Schettini
Diego Paternosto
Marta Salzillo
Roberto Alfano
Vincenzo Andreone
Lorenzo Salvatore Malatino
Gianni Biolo
Giuseppe Paolisso
Luigi Elio Adinolfi
author_facet Mauro Giordano
Maria Consiglia Trotta
Tiziana Ciarambino
Michele D’Amico
Marilena Galdiero
Federico Schettini
Diego Paternosto
Marta Salzillo
Roberto Alfano
Vincenzo Andreone
Lorenzo Salvatore Malatino
Gianni Biolo
Giuseppe Paolisso
Luigi Elio Adinolfi
author_sort Mauro Giordano
collection DOAJ
description (1) Background: Circulating micro-RNAs (miRNAs) modulate the expression of molecules in diabetes. We evaluated the expression of serum miRNA-195-5p and -451a in diabetic patients with ischemic stroke and correlated them with two markers of brain tissue integrity. (2) Methods: Seventy-eight subjects with acute ischemic stroke (AIS) or transient ischemic attack (TIA) (40 with diabetes) were enrolled. Serum miRNA levels, brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor A (VEGF-A) were assessed at admission and 24 and 72 h after a post-ischemic stroke, and were compared to 20 controls. (3) Results: Both circulating miRNAs were two-fold up-regulated in diabetic AIS and TIA patients compared to non-diabetics. Their levels progressively decreased at 24 and 72 h in both AIS and TIA patients. Interestingly, in the non-diabetic TIA group, both circulating miRNAs, although higher than the controls, tended to achieve a complete decay after 72 h. Furthermore, miRNA-195-5p and miRNA-451a levels inversely correlated with both BDNF and VEGF-A serum levels. (4) Conclusions: These data show a different profile of both micro-RNAs in diabetic versus non-diabetic patients after acute ischemic stroke, suggesting their pivotal role in cerebrovascular ischemic attack.
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spelling doaj.art-f640610b42604f0a8fe600916e59ae612023-11-20T17:09:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-012120761510.3390/ijms21207615Circulating MiRNA-195-5p and -451a in Diabetic Patients with Transient and Acute Ischemic Stroke in the Emergency DepartmentMauro Giordano0Maria Consiglia Trotta1Tiziana Ciarambino2Michele D’Amico3Marilena Galdiero4Federico Schettini5Diego Paternosto6Marta Salzillo7Roberto Alfano8Vincenzo Andreone9Lorenzo Salvatore Malatino10Gianni Biolo11Giuseppe Paolisso12Luigi Elio Adinolfi13Department of Advanced Medical and Surgical Sciences, University of Campania “L. Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, Division of Pharmacology, University of Campania “L. Vanvitelli”, 80138 Naples, ItalyDepartment of Internal Medicine, Hospital of Marcianise, ASL, 81025 Caserta, ItalyDepartment of Experimental Medicine, Division of Pharmacology, University of Campania “L. Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, Division of Pharmacology, University of Campania “L. Vanvitelli”, 80138 Naples, ItalyDepartment of Advanced Medical and Surgical Sciences, University of Campania “L. Vanvitelli”, 80138 Naples, ItalyDepartment of Emergency Medicine, AORN Sant’Anna e San Sebastiano,81100 Caserta, ItalyDepartment of Emergency Medicine, AORN Sant’Anna e San Sebastiano,81100 Caserta, ItalyDepartment of Experimental Medicine, Division of Pharmacology, University of Campania “L. Vanvitelli”, 80138 Naples, ItalyDepartment of Emergency Medicine, AORN Sant’Anna e San Sebastiano,81100 Caserta, ItalyDepartment of Clinical and Experimental Medicine, University of Catania, 95126 Catania, ItalyDepartment of Medical and Surgical Sciences, University of Trieste, 34100 Trieste, ItalyDepartment of Advanced Medical and Surgical Sciences, University of Campania “L. Vanvitelli”, 80138 Naples, ItalyDepartment of Advanced Medical and Surgical Sciences, University of Campania “L. Vanvitelli”, 80138 Naples, Italy(1) Background: Circulating micro-RNAs (miRNAs) modulate the expression of molecules in diabetes. We evaluated the expression of serum miRNA-195-5p and -451a in diabetic patients with ischemic stroke and correlated them with two markers of brain tissue integrity. (2) Methods: Seventy-eight subjects with acute ischemic stroke (AIS) or transient ischemic attack (TIA) (40 with diabetes) were enrolled. Serum miRNA levels, brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor A (VEGF-A) were assessed at admission and 24 and 72 h after a post-ischemic stroke, and were compared to 20 controls. (3) Results: Both circulating miRNAs were two-fold up-regulated in diabetic AIS and TIA patients compared to non-diabetics. Their levels progressively decreased at 24 and 72 h in both AIS and TIA patients. Interestingly, in the non-diabetic TIA group, both circulating miRNAs, although higher than the controls, tended to achieve a complete decay after 72 h. Furthermore, miRNA-195-5p and miRNA-451a levels inversely correlated with both BDNF and VEGF-A serum levels. (4) Conclusions: These data show a different profile of both micro-RNAs in diabetic versus non-diabetic patients after acute ischemic stroke, suggesting their pivotal role in cerebrovascular ischemic attack.https://www.mdpi.com/1422-0067/21/20/7615microRNAacute ischemic stroketransient ischemic attackdiabetes mellitusemergency
spellingShingle Mauro Giordano
Maria Consiglia Trotta
Tiziana Ciarambino
Michele D’Amico
Marilena Galdiero
Federico Schettini
Diego Paternosto
Marta Salzillo
Roberto Alfano
Vincenzo Andreone
Lorenzo Salvatore Malatino
Gianni Biolo
Giuseppe Paolisso
Luigi Elio Adinolfi
Circulating MiRNA-195-5p and -451a in Diabetic Patients with Transient and Acute Ischemic Stroke in the Emergency Department
International Journal of Molecular Sciences
microRNA
acute ischemic stroke
transient ischemic attack
diabetes mellitus
emergency
title Circulating MiRNA-195-5p and -451a in Diabetic Patients with Transient and Acute Ischemic Stroke in the Emergency Department
title_full Circulating MiRNA-195-5p and -451a in Diabetic Patients with Transient and Acute Ischemic Stroke in the Emergency Department
title_fullStr Circulating MiRNA-195-5p and -451a in Diabetic Patients with Transient and Acute Ischemic Stroke in the Emergency Department
title_full_unstemmed Circulating MiRNA-195-5p and -451a in Diabetic Patients with Transient and Acute Ischemic Stroke in the Emergency Department
title_short Circulating MiRNA-195-5p and -451a in Diabetic Patients with Transient and Acute Ischemic Stroke in the Emergency Department
title_sort circulating mirna 195 5p and 451a in diabetic patients with transient and acute ischemic stroke in the emergency department
topic microRNA
acute ischemic stroke
transient ischemic attack
diabetes mellitus
emergency
url https://www.mdpi.com/1422-0067/21/20/7615
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