QTL mapping and BSA-seq map a major QTL for the node of the first fruiting branch in cotton
Understanding the genetic basis of the node of the first fruiting branch (NFFB) improves early-maturity cotton breeding. Here we report QTL mapping on 200 F2 plants and derivative F2:3 and F2:4 populations by genotyping by sequencing (GBS). BC1F2 population was constructed by backcrossing one F2:4 l...
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| Format: | Article |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1113059/full |
| _version_ | 1797943682634088448 |
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| author | Xiaoyun Jia Shijie Wang Hongxia Zhao Jijie Zhu Miao Li Guoyin Wang |
| author_facet | Xiaoyun Jia Shijie Wang Hongxia Zhao Jijie Zhu Miao Li Guoyin Wang |
| author_sort | Xiaoyun Jia |
| collection | DOAJ |
| description | Understanding the genetic basis of the node of the first fruiting branch (NFFB) improves early-maturity cotton breeding. Here we report QTL mapping on 200 F2 plants and derivative F2:3 and F2:4 populations by genotyping by sequencing (GBS). BC1F2 population was constructed by backcrossing one F2:4 line with the maternal parent JF914 and used for BSA-seq for further QTL mapping. A total of 1,305,642 SNPs were developed between the parents by GBS, and 2,907,790 SNPs were detected by BSA-seq. A high-density genetic map was constructed containing 11,488 SNPs and spanning 4,202.12 cM in length. A total of 13 QTL were mapped in the 3 tested populations. JF914 conferred favorable alleles for 11 QTL, and JF173 conferred favorable alleles for the other 2 QTL. Two stable QTL were repeatedly mapped in F2:3 and F2:4, including qNFFB-D3-1 and qNFFB-D6-1. Only qNFFB-D3-1 contributed more than 10% of the phenotypic variation. This QTL covered about 24.7 Mb (17,130,008–41,839,226 bp) on chromosome D3. Two regions on D3 (41,779,195–41,836,120 bp, 41,836,768–41,872,287 bp) were found by BSA-seq and covered about 92.4 Kb. This 92.4 Kb region overlapped with the stable QTL qNFFB-D3-1 and contained 8 annotated genes. By qRT-PCR, Ghir_D03G012430 showed a lower expression level from the 1- to 2-leaf stage and a higher expression level from the 3- to 6-leaf stage in the buds of JF173 than that of JF914. Ghir_D03G012390 reached the highest level at the 3- and 5-leaf stages in the buds of JF173 and JF914, respectively. As JF173 has lower NFFB and more early maturity than JF914, these two genes might be important in cell division and differentiation during NFFB formation in the seedling stage. The results of this study will facilitate a better understanding of the genetic basis of NFFB and benefit cotton molecular breeding for improving earliness traits. |
| first_indexed | 2024-04-10T20:26:58Z |
| format | Article |
| id | doaj.art-f644253741b54f1796d7114c432a411c |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T20:26:58Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-f644253741b54f1796d7114c432a411c2023-01-25T08:54:49ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011410.3389/fpls.2023.11130591113059QTL mapping and BSA-seq map a major QTL for the node of the first fruiting branch in cottonXiaoyun JiaShijie WangHongxia ZhaoJijie ZhuMiao LiGuoyin WangUnderstanding the genetic basis of the node of the first fruiting branch (NFFB) improves early-maturity cotton breeding. Here we report QTL mapping on 200 F2 plants and derivative F2:3 and F2:4 populations by genotyping by sequencing (GBS). BC1F2 population was constructed by backcrossing one F2:4 line with the maternal parent JF914 and used for BSA-seq for further QTL mapping. A total of 1,305,642 SNPs were developed between the parents by GBS, and 2,907,790 SNPs were detected by BSA-seq. A high-density genetic map was constructed containing 11,488 SNPs and spanning 4,202.12 cM in length. A total of 13 QTL were mapped in the 3 tested populations. JF914 conferred favorable alleles for 11 QTL, and JF173 conferred favorable alleles for the other 2 QTL. Two stable QTL were repeatedly mapped in F2:3 and F2:4, including qNFFB-D3-1 and qNFFB-D6-1. Only qNFFB-D3-1 contributed more than 10% of the phenotypic variation. This QTL covered about 24.7 Mb (17,130,008–41,839,226 bp) on chromosome D3. Two regions on D3 (41,779,195–41,836,120 bp, 41,836,768–41,872,287 bp) were found by BSA-seq and covered about 92.4 Kb. This 92.4 Kb region overlapped with the stable QTL qNFFB-D3-1 and contained 8 annotated genes. By qRT-PCR, Ghir_D03G012430 showed a lower expression level from the 1- to 2-leaf stage and a higher expression level from the 3- to 6-leaf stage in the buds of JF173 than that of JF914. Ghir_D03G012390 reached the highest level at the 3- and 5-leaf stages in the buds of JF173 and JF914, respectively. As JF173 has lower NFFB and more early maturity than JF914, these two genes might be important in cell division and differentiation during NFFB formation in the seedling stage. The results of this study will facilitate a better understanding of the genetic basis of NFFB and benefit cotton molecular breeding for improving earliness traits.https://www.frontiersin.org/articles/10.3389/fpls.2023.1113059/fullcotton earlinessnode of the first fruiting branchQTL mappingBSA-seqcandidate gene |
| spellingShingle | Xiaoyun Jia Shijie Wang Hongxia Zhao Jijie Zhu Miao Li Guoyin Wang QTL mapping and BSA-seq map a major QTL for the node of the first fruiting branch in cotton Frontiers in Plant Science cotton earliness node of the first fruiting branch QTL mapping BSA-seq candidate gene |
| title | QTL mapping and BSA-seq map a major QTL for the node of the first fruiting branch in cotton |
| title_full | QTL mapping and BSA-seq map a major QTL for the node of the first fruiting branch in cotton |
| title_fullStr | QTL mapping and BSA-seq map a major QTL for the node of the first fruiting branch in cotton |
| title_full_unstemmed | QTL mapping and BSA-seq map a major QTL for the node of the first fruiting branch in cotton |
| title_short | QTL mapping and BSA-seq map a major QTL for the node of the first fruiting branch in cotton |
| title_sort | qtl mapping and bsa seq map a major qtl for the node of the first fruiting branch in cotton |
| topic | cotton earliness node of the first fruiting branch QTL mapping BSA-seq candidate gene |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1113059/full |
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