Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against Pathogenic <i>Vibrio coralliilyticus</i> Causing Mortality of Pacific Oyster (<i>Crassostrea gigas</i>) Larvae
<i>Vibrio coralliilyticus</i> is one of the major pathogens causing mass mortality in marine bivalve larvae aquaculture. To prevent and control <i>Vibrio</i> spp. infections in marine bivalve hatcheries, various antibiotics are overused, resulting in environmental pollution a...
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MDPI AG
2020-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/6/926 |
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| author | Hyoun Joong Kim Sib Sankar Giri Sang Guen Kim Sang Wha Kim Jun Kwon Sung Bin Lee Se Chang Park |
| author_facet | Hyoun Joong Kim Sib Sankar Giri Sang Guen Kim Sang Wha Kim Jun Kwon Sung Bin Lee Se Chang Park |
| author_sort | Hyoun Joong Kim |
| collection | DOAJ |
| description | <i>Vibrio coralliilyticus</i> is one of the major pathogens causing mass mortality in marine bivalve larvae aquaculture. To prevent and control <i>Vibrio</i> spp. infections in marine bivalve hatcheries, various antibiotics are overused, resulting in environmental pollution and the creation of multi-drug-resistant strains. Therefore, research on the development of antibiotic substitutes is required. In this study, we isolated two bacteriophages (phages) that specifically infected pathogenic <i>V. coralliilyticus</i> from an oyster hatchery and designated them as pVco-5 and pVco-7. Both phages were classified as <i>Podoviridae</i> and were stable over a wide range of temperatures (4–37 °C) and at pH 7.0–9.0. Thus, both phages were suitable for application under the environmental conditions of an oyster hatchery. The two phages showed confirmed significant bactericidal efficacy against pathogenic <i>V. coralliilyticus</i> in an in vitro test. In the in vivo experiment, the phage pre-treated groups of Pacific oyster larvae showed significantly lower mortality against <i>V. coralliilyticus</i> infection than untreated control larvae. The results of the present study suggest that both phages could be used in the artificial marine bivalve seedling industry; not only to prevent pathogenic <i>V. coralliilyticus</i> infection, but also to reduce antibiotic overuse. |
| first_indexed | 2024-03-10T19:02:45Z |
| format | Article |
| id | doaj.art-f645bbab747d410388ce0c9dc87266c0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T19:02:45Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f645bbab747d410388ce0c9dc87266c02023-11-20T04:21:39ZengMDPI AGMicroorganisms2076-26072020-06-018692610.3390/microorganisms8060926Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against Pathogenic <i>Vibrio coralliilyticus</i> Causing Mortality of Pacific Oyster (<i>Crassostrea gigas</i>) LarvaeHyoun Joong Kim0Sib Sankar Giri1Sang Guen Kim2Sang Wha Kim3Jun Kwon4Sung Bin Lee5Se Chang Park6Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea<i>Vibrio coralliilyticus</i> is one of the major pathogens causing mass mortality in marine bivalve larvae aquaculture. To prevent and control <i>Vibrio</i> spp. infections in marine bivalve hatcheries, various antibiotics are overused, resulting in environmental pollution and the creation of multi-drug-resistant strains. Therefore, research on the development of antibiotic substitutes is required. In this study, we isolated two bacteriophages (phages) that specifically infected pathogenic <i>V. coralliilyticus</i> from an oyster hatchery and designated them as pVco-5 and pVco-7. Both phages were classified as <i>Podoviridae</i> and were stable over a wide range of temperatures (4–37 °C) and at pH 7.0–9.0. Thus, both phages were suitable for application under the environmental conditions of an oyster hatchery. The two phages showed confirmed significant bactericidal efficacy against pathogenic <i>V. coralliilyticus</i> in an in vitro test. In the in vivo experiment, the phage pre-treated groups of Pacific oyster larvae showed significantly lower mortality against <i>V. coralliilyticus</i> infection than untreated control larvae. The results of the present study suggest that both phages could be used in the artificial marine bivalve seedling industry; not only to prevent pathogenic <i>V. coralliilyticus</i> infection, but also to reduce antibiotic overuse.https://www.mdpi.com/2076-2607/8/6/926Pacific oyster larvaemass mortality<i>Vibrio coralliilyticus</i>bacteriophagephage antibiotic substitution |
| spellingShingle | Hyoun Joong Kim Sib Sankar Giri Sang Guen Kim Sang Wha Kim Jun Kwon Sung Bin Lee Se Chang Park Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against Pathogenic <i>Vibrio coralliilyticus</i> Causing Mortality of Pacific Oyster (<i>Crassostrea gigas</i>) Larvae Microorganisms Pacific oyster larvae mass mortality <i>Vibrio coralliilyticus</i> bacteriophage phage antibiotic substitution |
| title | Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against Pathogenic <i>Vibrio coralliilyticus</i> Causing Mortality of Pacific Oyster (<i>Crassostrea gigas</i>) Larvae |
| title_full | Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against Pathogenic <i>Vibrio coralliilyticus</i> Causing Mortality of Pacific Oyster (<i>Crassostrea gigas</i>) Larvae |
| title_fullStr | Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against Pathogenic <i>Vibrio coralliilyticus</i> Causing Mortality of Pacific Oyster (<i>Crassostrea gigas</i>) Larvae |
| title_full_unstemmed | Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against Pathogenic <i>Vibrio coralliilyticus</i> Causing Mortality of Pacific Oyster (<i>Crassostrea gigas</i>) Larvae |
| title_short | Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against Pathogenic <i>Vibrio coralliilyticus</i> Causing Mortality of Pacific Oyster (<i>Crassostrea gigas</i>) Larvae |
| title_sort | isolation and characterization of two bacteriophages and their preventive effects against pathogenic i vibrio coralliilyticus i causing mortality of pacific oyster i crassostrea gigas i larvae |
| topic | Pacific oyster larvae mass mortality <i>Vibrio coralliilyticus</i> bacteriophage phage antibiotic substitution |
| url | https://www.mdpi.com/2076-2607/8/6/926 |
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