RAPD analysis of genetic diversity and qualitative assessment of hydrolytic activities in a collection of Bacillus sp. isolate

RAPD analysis of genetic diversity and qualitative assessment of hydrolytic activities in a collection of Bacillus sp. isolate

Genetic diversity and production of hydrolytic enzymes of 205 Bacillus isolates from different geographical and ecological niches in Serbia were studied. Combining RAPD analysis and 16S DNA sequencing, we determined 13 different groups of RAPD profiles within four (five) species: B. subtilis, B. cer...

Full description

Bibliographic Details
Main Authors: Berić Tanja, Urdaci Maria C., Stanković S., Knežević-Vukčević Jelena
Format: Article
Language:English
Published: University of Belgrade, University of Novi Sad 2009-01-01
Series:Archives of Biological Sciences
Subjects:
Bacillus isolates
RAPD
enzyme production
Online Access:http://www.doiserbia.nb.rs/img/doi/0354-4664/2009/0354-46640904645B.pdf
Description
Summary:Genetic diversity and production of hydrolytic enzymes of 205 Bacillus isolates from different geographical and ecological niches in Serbia were studied. Combining RAPD analysis and 16S DNA sequencing, we determined 13 different groups of RAPD profiles within four (five) species: B. subtilis, B. cereus/B. thuringiensis, B. pumilus, and B. firmus. Screening for production of hydrolytic enzymes showed that there was no correlation of enzyme production with species. Most of the isolates from all habitats produced amylase, protease, lipase, mannanase, and xylanase to some extent at 25ºC and 37ºC. The number of isolates that retained enzyme production ability at 55ºC is considerably lower and they predominantly came from manure.
ISSN:0354-4664

Similar Items