Optical Technologies for Single-Cell Analysis on Microchips
Cell analysis at the single-cell level is of great importance to investigate the inherent heterogeneity of cell populations and to understand the morphology, composition, and function of individual cells. With the continuous innovation of analytical techniques and methods, single-cell analysis on mi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/11/1/40 |
| _version_ | 1797444403461095424 |
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| author | Xiaowen Ou Peng Chen Bi-Feng Liu |
| author_facet | Xiaowen Ou Peng Chen Bi-Feng Liu |
| author_sort | Xiaowen Ou |
| collection | DOAJ |
| description | Cell analysis at the single-cell level is of great importance to investigate the inherent heterogeneity of cell populations and to understand the morphology, composition, and function of individual cells. With the continuous innovation of analytical techniques and methods, single-cell analysis on microfluidic chip systems has been extensively applied for its precise single-cell manipulation and sensitive signal response integrated with various detection techniques, such as optical, electrical, and mass spectrometric analyses. In this review, we focus on the specific optical events in single-cell analysis on a microfluidic chip system. First, the four most commonly applied optical technologies, i.e., fluorescence, surface-enhanced Raman spectroscopy, surface plasmon resonance, and interferometry, are briefly introduced. Then, we focus on the recent applications of the abovementioned optical technologies integrated with a microfluidic chip system for single-cell analysis. Finally, future directions of optical technologies for single-cell analysis on microfluidic chip systems are predicted. |
| first_indexed | 2024-03-09T13:12:07Z |
| format | Article |
| id | doaj.art-f648c7db268141bb8ae65436d18ab38c |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-09T13:12:07Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-f648c7db268141bb8ae65436d18ab38c2023-11-30T21:41:45ZengMDPI AGChemosensors2227-90402023-01-011114010.3390/chemosensors11010040Optical Technologies for Single-Cell Analysis on MicrochipsXiaowen Ou0Peng Chen1Bi-Feng Liu2Hubei Key Laboratory of Purification and Application of Plant Anti-Cancer Active Ingredients, Department of Chemistry and Life Science, Hubei University of Education, Wuhan 430205, ChinaThe Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaThe Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaCell analysis at the single-cell level is of great importance to investigate the inherent heterogeneity of cell populations and to understand the morphology, composition, and function of individual cells. With the continuous innovation of analytical techniques and methods, single-cell analysis on microfluidic chip systems has been extensively applied for its precise single-cell manipulation and sensitive signal response integrated with various detection techniques, such as optical, electrical, and mass spectrometric analyses. In this review, we focus on the specific optical events in single-cell analysis on a microfluidic chip system. First, the four most commonly applied optical technologies, i.e., fluorescence, surface-enhanced Raman spectroscopy, surface plasmon resonance, and interferometry, are briefly introduced. Then, we focus on the recent applications of the abovementioned optical technologies integrated with a microfluidic chip system for single-cell analysis. Finally, future directions of optical technologies for single-cell analysis on microfluidic chip systems are predicted.https://www.mdpi.com/2227-9040/11/1/40single-cell analysismicrofluidic chipfluorescenceSERSsurface plasmon resonanceinterferometry |
| spellingShingle | Xiaowen Ou Peng Chen Bi-Feng Liu Optical Technologies for Single-Cell Analysis on Microchips Chemosensors single-cell analysis microfluidic chip fluorescence SERS surface plasmon resonance interferometry |
| title | Optical Technologies for Single-Cell Analysis on Microchips |
| title_full | Optical Technologies for Single-Cell Analysis on Microchips |
| title_fullStr | Optical Technologies for Single-Cell Analysis on Microchips |
| title_full_unstemmed | Optical Technologies for Single-Cell Analysis on Microchips |
| title_short | Optical Technologies for Single-Cell Analysis on Microchips |
| title_sort | optical technologies for single cell analysis on microchips |
| topic | single-cell analysis microfluidic chip fluorescence SERS surface plasmon resonance interferometry |
| url | https://www.mdpi.com/2227-9040/11/1/40 |
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