Single Cell Transcriptomics: Methods and Applications
Traditionally, gene expression measurements were performed on bulk samples containing populations of thousands of cells. Recent advances in genomic technology have made it possible to measure gene expression in hundreds of individual cells at a time. As a result, cellular properties that were previo...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-03-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fonc.2015.00053/full |
| _version_ | 1811234070879797248 |
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| author | Itamar eKanter Tomer eKalisky |
| author_facet | Itamar eKanter Tomer eKalisky |
| author_sort | Itamar eKanter |
| collection | DOAJ |
| description | Traditionally, gene expression measurements were performed on bulk samples containing populations of thousands of cells. Recent advances in genomic technology have made it possible to measure gene expression in hundreds of individual cells at a time. As a result, cellular properties that were previously masked in bulk measurements can now be observed directly. In this review we will survey emerging technologies for single cell transcriptomics, and describe how they are used to study complex disease such as cancer, as well as other biological phenomena such as tissue regeneration, embryonic development, and immune response. |
| first_indexed | 2024-04-12T11:30:26Z |
| format | Article |
| id | doaj.art-f649c878e850412a811036ef14e6f3b5 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-12T11:30:26Z |
| publishDate | 2015-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-f649c878e850412a811036ef14e6f3b52022-12-22T03:35:02ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2015-03-01510.3389/fonc.2015.00053129637Single Cell Transcriptomics: Methods and ApplicationsItamar eKanter0Tomer eKalisky1Bar-Ilan universityBar-Ilan universityTraditionally, gene expression measurements were performed on bulk samples containing populations of thousands of cells. Recent advances in genomic technology have made it possible to measure gene expression in hundreds of individual cells at a time. As a result, cellular properties that were previously masked in bulk measurements can now be observed directly. In this review we will survey emerging technologies for single cell transcriptomics, and describe how they are used to study complex disease such as cancer, as well as other biological phenomena such as tissue regeneration, embryonic development, and immune response.http://journal.frontiersin.org/Journal/10.3389/fonc.2015.00053/fullGene ExpressionqPCRfishsingle cellRNA sequencing |
| spellingShingle | Itamar eKanter Tomer eKalisky Single Cell Transcriptomics: Methods and Applications Frontiers in Oncology Gene Expression qPCR fish single cell RNA sequencing |
| title | Single Cell Transcriptomics: Methods and Applications |
| title_full | Single Cell Transcriptomics: Methods and Applications |
| title_fullStr | Single Cell Transcriptomics: Methods and Applications |
| title_full_unstemmed | Single Cell Transcriptomics: Methods and Applications |
| title_short | Single Cell Transcriptomics: Methods and Applications |
| title_sort | single cell transcriptomics methods and applications |
| topic | Gene Expression qPCR fish single cell RNA sequencing |
| url | http://journal.frontiersin.org/Journal/10.3389/fonc.2015.00053/full |
| work_keys_str_mv | AT itamarekanter singlecelltranscriptomicsmethodsandapplications AT tomerekalisky singlecelltranscriptomicsmethodsandapplications |