Acinetobacter Plasmids: Diversity and Development of Classification Strategies
Bacteria of the genus Acinetobacter, with their numerous species common in various habitats, play a significant role as pathogens. Their ability to adapt to different living conditions is largely due to the presence of numerous plasmids containing the necessary adaptive genes. At the same time the d...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.588410/full |
| _version_ | 1818341401230835712 |
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| author | Sofia Mindlin Alexey Beletsky Andrey Rakitin Andrey Mardanov Mayya Petrova |
| author_facet | Sofia Mindlin Alexey Beletsky Andrey Rakitin Andrey Mardanov Mayya Petrova |
| author_sort | Sofia Mindlin |
| collection | DOAJ |
| description | Bacteria of the genus Acinetobacter, with their numerous species common in various habitats, play a significant role as pathogens. Their ability to adapt to different living conditions is largely due to the presence of numerous plasmids containing the necessary adaptive genes. At the same time the diversity of Acinetobacter plasmids and their evolutionary dynamics have not been sufficiently studied. Here, we characterized 44 plasmids isolated from five permafrost Acinetobacter lwoffii strains, examined their relationship with plasmids of modern Acinetobacter strains and identified groups of related plasmids. For this purpose, we have developed a combined approach for classifying all known Acinetobacter plasmids. The classification took into account the size of plasmids, the presence and structure of the rep and mob genes, as well as the structure of their backbone and accessory regions. Based on the analysis, 19 major groups (lineages) of plasmids were identified, of which more than half were small plasmids. The plasmids of each group have common features of the organization of the backbone region with a DNA identity level of at least 80%. In addition, plasmids of the same group have similarities in the organization of accessory regions. We also described a number of plasmids with a unique structure. The presence of plasmids in clinical strains that are closely related to those of environmental permafrost strains provides evidence of the origin of the former from the latter. |
| first_indexed | 2024-12-13T15:58:12Z |
| format | Article |
| id | doaj.art-f64cb9cc75c84db69646f3836dbf9ccc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T15:58:12Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f64cb9cc75c84db69646f3836dbf9ccc2022-12-21T23:39:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-11-011110.3389/fmicb.2020.588410588410Acinetobacter Plasmids: Diversity and Development of Classification StrategiesSofia Mindlin0Alexey Beletsky1Andrey Rakitin2Andrey Mardanov3Mayya Petrova4Institute of Molecular Genetics of National Research Centre “Kurchatov Institute”, Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, RussiaInstitute of Molecular Genetics of National Research Centre “Kurchatov Institute”, Moscow, RussiaBacteria of the genus Acinetobacter, with their numerous species common in various habitats, play a significant role as pathogens. Their ability to adapt to different living conditions is largely due to the presence of numerous plasmids containing the necessary adaptive genes. At the same time the diversity of Acinetobacter plasmids and their evolutionary dynamics have not been sufficiently studied. Here, we characterized 44 plasmids isolated from five permafrost Acinetobacter lwoffii strains, examined their relationship with plasmids of modern Acinetobacter strains and identified groups of related plasmids. For this purpose, we have developed a combined approach for classifying all known Acinetobacter plasmids. The classification took into account the size of plasmids, the presence and structure of the rep and mob genes, as well as the structure of their backbone and accessory regions. Based on the analysis, 19 major groups (lineages) of plasmids were identified, of which more than half were small plasmids. The plasmids of each group have common features of the organization of the backbone region with a DNA identity level of at least 80%. In addition, plasmids of the same group have similarities in the organization of accessory regions. We also described a number of plasmids with a unique structure. The presence of plasmids in clinical strains that are closely related to those of environmental permafrost strains provides evidence of the origin of the former from the latter.https://www.frontiersin.org/articles/10.3389/fmicb.2020.588410/fullrelaxasesreplication initiator proteinsbackbone and accessory regionsphylogenetic analysisadaptation of bacteria |
| spellingShingle | Sofia Mindlin Alexey Beletsky Andrey Rakitin Andrey Mardanov Mayya Petrova Acinetobacter Plasmids: Diversity and Development of Classification Strategies Frontiers in Microbiology relaxases replication initiator proteins backbone and accessory regions phylogenetic analysis adaptation of bacteria |
| title | Acinetobacter Plasmids: Diversity and Development of Classification Strategies |
| title_full | Acinetobacter Plasmids: Diversity and Development of Classification Strategies |
| title_fullStr | Acinetobacter Plasmids: Diversity and Development of Classification Strategies |
| title_full_unstemmed | Acinetobacter Plasmids: Diversity and Development of Classification Strategies |
| title_short | Acinetobacter Plasmids: Diversity and Development of Classification Strategies |
| title_sort | acinetobacter plasmids diversity and development of classification strategies |
| topic | relaxases replication initiator proteins backbone and accessory regions phylogenetic analysis adaptation of bacteria |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.588410/full |
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