Efficient Delivery of Therapeutic siRNA by Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles into Oral Cancer Cells
The incidence of oral cancer is increasing due to smoking, drinking, and human papillomavirus (HPV) infection, while the current treatments are not satisfactory. Small interfering RNA (siRNA)-based therapy has brought hope, but an efficient delivery system is still needed. Here, polyethyleneimine (P...
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MDPI AG
2019-11-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/11/11/615 |
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| author | Lili Jin Qiuyu Wang Jiayu Chen Zixiang Wang Hongchuan Xin Dianbao Zhang |
| author_facet | Lili Jin Qiuyu Wang Jiayu Chen Zixiang Wang Hongchuan Xin Dianbao Zhang |
| author_sort | Lili Jin |
| collection | DOAJ |
| description | The incidence of oral cancer is increasing due to smoking, drinking, and human papillomavirus (HPV) infection, while the current treatments are not satisfactory. Small interfering RNA (siRNA)-based therapy has brought hope, but an efficient delivery system is still needed. Here, polyethyleneimine (PEI)-modified magnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles were prepared for the delivery of therapeutic siRNAs targeting B-cell lymphoma-2 (BCL2) and Baculoviral IAP repeat-containing 5 (BIRC5) into Ca9-22 oral cancer cells. The cationic nanoparticles were characterized by transmission electronic microscopy (TEM), scanning electronic microscopy (SEM), dynamic light scattering (DLS), and vibrating sample magnetometer (VSM). By gel retardation assay, the nanoparticles were found to block siRNA in a concentration-dependent manner. The cellular uptake of the nanoparticle/siRNA complexes under a magnetic field was visualized by Perl’s Prussian blue staining and FAM labeling. High gene silencing efficiencies were determined by quantitative real-time PCR and western blotting. Furthermore, the nanoparticle-delivered siRNAs targeting BCL2 and BIRC5 were found to remarkably inhibit the viability and migration of Ca9-22 cells, by cell counting kit-8 assay and transwell assay. In this study, we have developed a novel siRNA-based therapeutic strategy targeting BCL2 and BIRC5 for oral cancer. |
| first_indexed | 2024-04-11T12:14:30Z |
| format | Article |
| id | doaj.art-f64eae390a4f483cb7b6ea2bf5fafde1 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-11T12:14:30Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-f64eae390a4f483cb7b6ea2bf5fafde12022-12-22T04:24:27ZengMDPI AGPharmaceutics1999-49232019-11-01111161510.3390/pharmaceutics11110615pharmaceutics11110615Efficient Delivery of Therapeutic siRNA by Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles into Oral Cancer CellsLili Jin0Qiuyu Wang1Jiayu Chen2Zixiang Wang3Hongchuan Xin4Dianbao Zhang5School of Life Science, Liaoning University, Shenyang 110036, ChinaSchool of Life Science, Liaoning University, Shenyang 110036, ChinaDepartment of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology, National Health Commission of China, and Key Laboratory of Medical Cell Biology, Ministry of Education of China, China Medical University, Shenyang 110122, ChinaSchool of Life Science, Liaoning University, Shenyang 110036, ChinaQingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, ChinaDepartment of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology, National Health Commission of China, and Key Laboratory of Medical Cell Biology, Ministry of Education of China, China Medical University, Shenyang 110122, ChinaThe incidence of oral cancer is increasing due to smoking, drinking, and human papillomavirus (HPV) infection, while the current treatments are not satisfactory. Small interfering RNA (siRNA)-based therapy has brought hope, but an efficient delivery system is still needed. Here, polyethyleneimine (PEI)-modified magnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles were prepared for the delivery of therapeutic siRNAs targeting B-cell lymphoma-2 (BCL2) and Baculoviral IAP repeat-containing 5 (BIRC5) into Ca9-22 oral cancer cells. The cationic nanoparticles were characterized by transmission electronic microscopy (TEM), scanning electronic microscopy (SEM), dynamic light scattering (DLS), and vibrating sample magnetometer (VSM). By gel retardation assay, the nanoparticles were found to block siRNA in a concentration-dependent manner. The cellular uptake of the nanoparticle/siRNA complexes under a magnetic field was visualized by Perl’s Prussian blue staining and FAM labeling. High gene silencing efficiencies were determined by quantitative real-time PCR and western blotting. Furthermore, the nanoparticle-delivered siRNAs targeting BCL2 and BIRC5 were found to remarkably inhibit the viability and migration of Ca9-22 cells, by cell counting kit-8 assay and transwell assay. In this study, we have developed a novel siRNA-based therapeutic strategy targeting BCL2 and BIRC5 for oral cancer.https://www.mdpi.com/1999-4923/11/11/615magnetic nanoparticleiron oxidesirna deliverybcl2birc5/survivinoral cancer |
| spellingShingle | Lili Jin Qiuyu Wang Jiayu Chen Zixiang Wang Hongchuan Xin Dianbao Zhang Efficient Delivery of Therapeutic siRNA by Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles into Oral Cancer Cells Pharmaceutics magnetic nanoparticle iron oxide sirna delivery bcl2 birc5/survivin oral cancer |
| title | Efficient Delivery of Therapeutic siRNA by Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles into Oral Cancer Cells |
| title_full | Efficient Delivery of Therapeutic siRNA by Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles into Oral Cancer Cells |
| title_fullStr | Efficient Delivery of Therapeutic siRNA by Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles into Oral Cancer Cells |
| title_full_unstemmed | Efficient Delivery of Therapeutic siRNA by Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles into Oral Cancer Cells |
| title_short | Efficient Delivery of Therapeutic siRNA by Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles into Oral Cancer Cells |
| title_sort | efficient delivery of therapeutic sirna by fe sub 3 sub o sub 4 sub magnetic nanoparticles into oral cancer cells |
| topic | magnetic nanoparticle iron oxide sirna delivery bcl2 birc5/survivin oral cancer |
| url | https://www.mdpi.com/1999-4923/11/11/615 |
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