Efficient gene knockout in goats using CRISPR/Cas9 system.
The CRISPR/Cas9 system has been adapted as an efficient genome editing tool in laboratory animals such as mice, rats, zebrafish and pigs. Here, we report that CRISPR/Cas9 mediated approach can efficiently induce monoallelic and biallelic gene knockout in goat primary fibroblasts. Four genes were dis...
| Main Authors: | , , , , , , , |
|---|---|
| 格式: | 文件 |
| 语言: | English |
| 出版: |
Public Library of Science (PLoS)
2014-01-01
|
| 丛编: | PLoS ONE |
| 在线阅读: | http://europepmc.org/articles/PMC4154755?pdf=render |