Comparative transcriptomic analysis of transcription factors and hormones during flower bud differentiation in ‘Red Globe’ grape under red‒blue light
Abstract Grape is a globally significant fruit-bearing crop, and the grape flower bud differentiation essential to fruit production is closely related to light quality. To investigate the regulatory mechanism of grape flower bud differentiation under red‒blue light, the transcriptome and hormone con...
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| Format: | Article |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-29402-5 |
| _version_ | 1797811517331079168 |
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| author | Xin Liu Miao Yuan Shizhuo Dang Juan Zhou Yahong Zhang |
| author_facet | Xin Liu Miao Yuan Shizhuo Dang Juan Zhou Yahong Zhang |
| author_sort | Xin Liu |
| collection | DOAJ |
| description | Abstract Grape is a globally significant fruit-bearing crop, and the grape flower bud differentiation essential to fruit production is closely related to light quality. To investigate the regulatory mechanism of grape flower bud differentiation under red‒blue light, the transcriptome and hormone content were determined at four stages of flower bud differentiation. The levels of indole-3-acetic acid (IAA) and abscisic acid (ABA) in grape flower buds at all stages of differentiation under red‒blue light were higher than those in the control. However, the levels of cytokinins (CKs) and gibberellic acid (giberellins, GAs) fluctuated continuously over the course of flower bud differentiation. Moreover, many differentially expressed genes were involved in auxin, CK, GA, and the ABA signal transduction pathways. There were significant differences in the AUX/IAA, SAUR, A-RR, and ABF gene expression levels between the red‒blue light treatment and the control buds, especially in regard to the ABF genes, the expression levels of which were completely different between the two groups. The expression of GBF4 and AI5L2 in the control was always low, while the expression under red‒blue light increased. AI5L7 and AI5L5 expression levels showed an upwards trend in the control plant buds and gradually decreased in red‒blue light treatment plant buds. Through weighted gene coexpression network analysis, we determined that the transcription factors WRK48 (WRKY family), EF110 (ERF family), ABR1, CAMTA3 (CAMTA family), and HSFA3 (HSF family) may be involved in the regulation of the GBF4 gene. This study lays a foundation for further analysis of grape flower bud differentiation regulation under red‒blue light. |
| first_indexed | 2024-03-13T07:24:52Z |
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| id | doaj.art-f6514762f45c48da826153e4cfb3156d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T07:24:52Z |
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| spelling | doaj.art-f6514762f45c48da826153e4cfb3156d2023-06-04T11:28:26ZengNature PortfolioScientific Reports2045-23222023-06-0113111210.1038/s41598-023-29402-5Comparative transcriptomic analysis of transcription factors and hormones during flower bud differentiation in ‘Red Globe’ grape under red‒blue lightXin Liu0Miao Yuan1Shizhuo Dang2Juan Zhou3Yahong Zhang4College of Agriculture, Ningxia UniversityCollege of Agriculture, Ningxia UniversityCollege of Agriculture, Ningxia UniversityCollege of Agriculture, Ningxia UniversityCollege of Agriculture, Ningxia UniversityAbstract Grape is a globally significant fruit-bearing crop, and the grape flower bud differentiation essential to fruit production is closely related to light quality. To investigate the regulatory mechanism of grape flower bud differentiation under red‒blue light, the transcriptome and hormone content were determined at four stages of flower bud differentiation. The levels of indole-3-acetic acid (IAA) and abscisic acid (ABA) in grape flower buds at all stages of differentiation under red‒blue light were higher than those in the control. However, the levels of cytokinins (CKs) and gibberellic acid (giberellins, GAs) fluctuated continuously over the course of flower bud differentiation. Moreover, many differentially expressed genes were involved in auxin, CK, GA, and the ABA signal transduction pathways. There were significant differences in the AUX/IAA, SAUR, A-RR, and ABF gene expression levels between the red‒blue light treatment and the control buds, especially in regard to the ABF genes, the expression levels of which were completely different between the two groups. The expression of GBF4 and AI5L2 in the control was always low, while the expression under red‒blue light increased. AI5L7 and AI5L5 expression levels showed an upwards trend in the control plant buds and gradually decreased in red‒blue light treatment plant buds. Through weighted gene coexpression network analysis, we determined that the transcription factors WRK48 (WRKY family), EF110 (ERF family), ABR1, CAMTA3 (CAMTA family), and HSFA3 (HSF family) may be involved in the regulation of the GBF4 gene. This study lays a foundation for further analysis of grape flower bud differentiation regulation under red‒blue light.https://doi.org/10.1038/s41598-023-29402-5 |
| spellingShingle | Xin Liu Miao Yuan Shizhuo Dang Juan Zhou Yahong Zhang Comparative transcriptomic analysis of transcription factors and hormones during flower bud differentiation in ‘Red Globe’ grape under red‒blue light Scientific Reports |
| title | Comparative transcriptomic analysis of transcription factors and hormones during flower bud differentiation in ‘Red Globe’ grape under red‒blue light |
| title_full | Comparative transcriptomic analysis of transcription factors and hormones during flower bud differentiation in ‘Red Globe’ grape under red‒blue light |
| title_fullStr | Comparative transcriptomic analysis of transcription factors and hormones during flower bud differentiation in ‘Red Globe’ grape under red‒blue light |
| title_full_unstemmed | Comparative transcriptomic analysis of transcription factors and hormones during flower bud differentiation in ‘Red Globe’ grape under red‒blue light |
| title_short | Comparative transcriptomic analysis of transcription factors and hormones during flower bud differentiation in ‘Red Globe’ grape under red‒blue light |
| title_sort | comparative transcriptomic analysis of transcription factors and hormones during flower bud differentiation in red globe grape under red blue light |
| url | https://doi.org/10.1038/s41598-023-29402-5 |
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