Culture Age, Growth Medium, Ultrasound Amplitude, and Time of Exposure Influence the Kinetic Growth of <i>Lactobacillus acidophilus</i>
The growth pattern of probiotics can be modified by changing their nutritional factors and their physiological stage. Meanwhile, high intensity ultrasound (HIUS) can be employed to increase probiotics’ biomass. The one-factor-at-a-time (OFAT) approach was employed to investigate the influence of the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/1/63 |
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| author | Norma Angélica Bolivar-Jacobo Raúl Alberto Reyes-Villagrana Ana Luisa Rentería-Monterrubio Rogelio Sánchez-Vega Eduardo Santellano-Estrada Juan Manuel Tirado-Gallegos América Chávez-Martínez |
| author_facet | Norma Angélica Bolivar-Jacobo Raúl Alberto Reyes-Villagrana Ana Luisa Rentería-Monterrubio Rogelio Sánchez-Vega Eduardo Santellano-Estrada Juan Manuel Tirado-Gallegos América Chávez-Martínez |
| author_sort | Norma Angélica Bolivar-Jacobo |
| collection | DOAJ |
| description | The growth pattern of probiotics can be modified by changing their nutritional factors and their physiological stage. Meanwhile, high intensity ultrasound (HIUS) can be employed to increase probiotics’ biomass. The one-factor-at-a-time (OFAT) approach was employed to investigate the influence of the growth medium (MRS broth, whole milk, and skim milk), culture age (1 day and 7 days old) and ultrasound parameters (time and amplitude) on the kinetic parameters of <i>L. acidophilus</i>. The oldest culture (7 days) had a greater lag phase and time to reach the end of the sigmoidal curve (T<sub>max</sub>) (<i>p</i> < 0.05) as well as a lower rate (maximum growth potential μ<sub>max</sub>) compared to the youngest culture (1 day). Regarding the growth medium, skim milk presented the greatest <i>L. acidophilus</i> counts (<i>p</i> < 0.05). Meanwhile, sonication times (60 and 90 s) change µ<sub>max</sub> and T<sub>max</sub>. When 30% amplitude was applied, a greater μ<sub>max</sub> and a smaller T<sub>max</sub> were observed (<i>p</i> < 0.05). It can be concluded that the growth medium, culture age, and ultrasound parameters (time and amplitude) influence the kinetic parameters of <i>L. acidophilus.</i> Results from this study could be used in the design and optimization of processes to improve the growth of the probiotic <i>L. acidophilus</i> at industrial scale. |
| first_indexed | 2024-03-09T12:47:29Z |
| format | Article |
| id | doaj.art-f651e27702e84a9ea58e72fb67fa96aa |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T12:47:29Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-f651e27702e84a9ea58e72fb67fa96aa2023-11-30T22:10:58ZengMDPI AGFermentation2311-56372023-01-01916310.3390/fermentation9010063Culture Age, Growth Medium, Ultrasound Amplitude, and Time of Exposure Influence the Kinetic Growth of <i>Lactobacillus acidophilus</i>Norma Angélica Bolivar-Jacobo0Raúl Alberto Reyes-Villagrana1Ana Luisa Rentería-Monterrubio2Rogelio Sánchez-Vega3Eduardo Santellano-Estrada4Juan Manuel Tirado-Gallegos5América Chávez-Martínez6Facultad de Zootécnica y Ecología, Universidad Autónoma de Chihuahua, Periférico Francisco R. Almada, Km 1, Chihuahua 31453, MexicoConsejo Nacional de Ciencia y Tecnología, Av. Insurgentes Sur 1582, Col. Crédito Constructor, Col. Benito Juárez, Ciudad de México 03940, MexicoFacultad de Zootécnica y Ecología, Universidad Autónoma de Chihuahua, Periférico Francisco R. Almada, Km 1, Chihuahua 31453, MexicoFacultad de Zootécnica y Ecología, Universidad Autónoma de Chihuahua, Periférico Francisco R. Almada, Km 1, Chihuahua 31453, MexicoFacultad de Zootécnica y Ecología, Universidad Autónoma de Chihuahua, Periférico Francisco R. Almada, Km 1, Chihuahua 31453, MexicoFacultad de Zootécnica y Ecología, Universidad Autónoma de Chihuahua, Periférico Francisco R. Almada, Km 1, Chihuahua 31453, MexicoFacultad de Zootécnica y Ecología, Universidad Autónoma de Chihuahua, Periférico Francisco R. Almada, Km 1, Chihuahua 31453, MexicoThe growth pattern of probiotics can be modified by changing their nutritional factors and their physiological stage. Meanwhile, high intensity ultrasound (HIUS) can be employed to increase probiotics’ biomass. The one-factor-at-a-time (OFAT) approach was employed to investigate the influence of the growth medium (MRS broth, whole milk, and skim milk), culture age (1 day and 7 days old) and ultrasound parameters (time and amplitude) on the kinetic parameters of <i>L. acidophilus</i>. The oldest culture (7 days) had a greater lag phase and time to reach the end of the sigmoidal curve (T<sub>max</sub>) (<i>p</i> < 0.05) as well as a lower rate (maximum growth potential μ<sub>max</sub>) compared to the youngest culture (1 day). Regarding the growth medium, skim milk presented the greatest <i>L. acidophilus</i> counts (<i>p</i> < 0.05). Meanwhile, sonication times (60 and 90 s) change µ<sub>max</sub> and T<sub>max</sub>. When 30% amplitude was applied, a greater μ<sub>max</sub> and a smaller T<sub>max</sub> were observed (<i>p</i> < 0.05). It can be concluded that the growth medium, culture age, and ultrasound parameters (time and amplitude) influence the kinetic parameters of <i>L. acidophilus.</i> Results from this study could be used in the design and optimization of processes to improve the growth of the probiotic <i>L. acidophilus</i> at industrial scale.https://www.mdpi.com/2311-5637/9/1/63ultrasound<i>Lactobacillus acidophilus</i>kinetic parameters |
| spellingShingle | Norma Angélica Bolivar-Jacobo Raúl Alberto Reyes-Villagrana Ana Luisa Rentería-Monterrubio Rogelio Sánchez-Vega Eduardo Santellano-Estrada Juan Manuel Tirado-Gallegos América Chávez-Martínez Culture Age, Growth Medium, Ultrasound Amplitude, and Time of Exposure Influence the Kinetic Growth of <i>Lactobacillus acidophilus</i> Fermentation ultrasound <i>Lactobacillus acidophilus</i> kinetic parameters |
| title | Culture Age, Growth Medium, Ultrasound Amplitude, and Time of Exposure Influence the Kinetic Growth of <i>Lactobacillus acidophilus</i> |
| title_full | Culture Age, Growth Medium, Ultrasound Amplitude, and Time of Exposure Influence the Kinetic Growth of <i>Lactobacillus acidophilus</i> |
| title_fullStr | Culture Age, Growth Medium, Ultrasound Amplitude, and Time of Exposure Influence the Kinetic Growth of <i>Lactobacillus acidophilus</i> |
| title_full_unstemmed | Culture Age, Growth Medium, Ultrasound Amplitude, and Time of Exposure Influence the Kinetic Growth of <i>Lactobacillus acidophilus</i> |
| title_short | Culture Age, Growth Medium, Ultrasound Amplitude, and Time of Exposure Influence the Kinetic Growth of <i>Lactobacillus acidophilus</i> |
| title_sort | culture age growth medium ultrasound amplitude and time of exposure influence the kinetic growth of i lactobacillus acidophilus i |
| topic | ultrasound <i>Lactobacillus acidophilus</i> kinetic parameters |
| url | https://www.mdpi.com/2311-5637/9/1/63 |
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