Effect of BIO-PLY<sup>TM</sup>, a Platelet-Rich Plasma Derived Biologic on PRRSV-2-Infected Macrophages
Porcine Reproductive and Respiratory Syndrome (PRRS) is the one of the most devastating diseases impacting the swine industry worldwide. Control and prevention methods rely on biosafety measures and vaccination. As an RNA virus with a high rate of mutation, vaccines are only partially effective agai...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/12/2666 |
| _version_ | 1827636616711110656 |
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| author | Alba Frias-De-Diego Jessica M. Gilbertie Frank Scholle Sarah Dejarnette Elisa Crisci |
| author_facet | Alba Frias-De-Diego Jessica M. Gilbertie Frank Scholle Sarah Dejarnette Elisa Crisci |
| author_sort | Alba Frias-De-Diego |
| collection | DOAJ |
| description | Porcine Reproductive and Respiratory Syndrome (PRRS) is the one of the most devastating diseases impacting the swine industry worldwide. Control and prevention methods rely on biosafety measures and vaccination. As an RNA virus with a high rate of mutation, vaccines are only partially effective against circulating and newly emerging strains. To reduce the burden of this disease, research on alternative control methods is needed. Here, we assess the in vitro antiviral effect of a novel platelet-rich plasma-derived biologic termed BIO-PLY<sup>TM</sup> (for the BIOactive fraction of Platelet-rich plasma LYsate) from both swine and equine origin. Our results show that BIO-PLY<sup>TM</sup> significantly reduces the amount of PRRSV viral load determined by RT-qPCR and the number of infectious viral particles measured by TCID50 in infected porcine alveolar and parenchymal macrophages. This study also showed limited toxicity of BIO-PLY<sup>TM</sup> in vitro and aspects of its immunomodulatory capacity evaluating the regulation of reactive oxygen species and cytokines production in infected cells. Finally, this study presents promising data on the effect of BIO-PLY<sup>TM</sup> on other RNA viruses such as human A influenza viruses and coronavirus. |
| first_indexed | 2024-03-09T15:45:18Z |
| format | Article |
| id | doaj.art-f6521a3a3ce8452aa787eef1c14ec1c9 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T15:45:18Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f6521a3a3ce8452aa787eef1c14ec1c92023-11-24T18:37:29ZengMDPI AGViruses1999-49152022-11-011412266610.3390/v14122666Effect of BIO-PLY<sup>TM</sup>, a Platelet-Rich Plasma Derived Biologic on PRRSV-2-Infected MacrophagesAlba Frias-De-Diego0Jessica M. Gilbertie1Frank Scholle2Sarah Dejarnette3Elisa Crisci4Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USADepartment of Biomedical Affairs and Research, Edward Via College of Osteopathic Medicine, Blacksburg, VA 24060, USADepartment of Biological Sciences, North Carolina State University, Raleigh, NC 27695, USADepartment of Biological Sciences, North Carolina State University, Raleigh, NC 27695, USADepartment of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USAPorcine Reproductive and Respiratory Syndrome (PRRS) is the one of the most devastating diseases impacting the swine industry worldwide. Control and prevention methods rely on biosafety measures and vaccination. As an RNA virus with a high rate of mutation, vaccines are only partially effective against circulating and newly emerging strains. To reduce the burden of this disease, research on alternative control methods is needed. Here, we assess the in vitro antiviral effect of a novel platelet-rich plasma-derived biologic termed BIO-PLY<sup>TM</sup> (for the BIOactive fraction of Platelet-rich plasma LYsate) from both swine and equine origin. Our results show that BIO-PLY<sup>TM</sup> significantly reduces the amount of PRRSV viral load determined by RT-qPCR and the number of infectious viral particles measured by TCID50 in infected porcine alveolar and parenchymal macrophages. This study also showed limited toxicity of BIO-PLY<sup>TM</sup> in vitro and aspects of its immunomodulatory capacity evaluating the regulation of reactive oxygen species and cytokines production in infected cells. Finally, this study presents promising data on the effect of BIO-PLY<sup>TM</sup> on other RNA viruses such as human A influenza viruses and coronavirus.https://www.mdpi.com/1999-4915/14/12/2666antiviralsbiologicporcine macrophagesPRRSV |
| spellingShingle | Alba Frias-De-Diego Jessica M. Gilbertie Frank Scholle Sarah Dejarnette Elisa Crisci Effect of BIO-PLY<sup>TM</sup>, a Platelet-Rich Plasma Derived Biologic on PRRSV-2-Infected Macrophages Viruses antivirals biologic porcine macrophages PRRSV |
| title | Effect of BIO-PLY<sup>TM</sup>, a Platelet-Rich Plasma Derived Biologic on PRRSV-2-Infected Macrophages |
| title_full | Effect of BIO-PLY<sup>TM</sup>, a Platelet-Rich Plasma Derived Biologic on PRRSV-2-Infected Macrophages |
| title_fullStr | Effect of BIO-PLY<sup>TM</sup>, a Platelet-Rich Plasma Derived Biologic on PRRSV-2-Infected Macrophages |
| title_full_unstemmed | Effect of BIO-PLY<sup>TM</sup>, a Platelet-Rich Plasma Derived Biologic on PRRSV-2-Infected Macrophages |
| title_short | Effect of BIO-PLY<sup>TM</sup>, a Platelet-Rich Plasma Derived Biologic on PRRSV-2-Infected Macrophages |
| title_sort | effect of bio ply sup tm sup a platelet rich plasma derived biologic on prrsv 2 infected macrophages |
| topic | antivirals biologic porcine macrophages PRRSV |
| url | https://www.mdpi.com/1999-4915/14/12/2666 |
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