High-Throughput Microbial Community Analyses to Establish a Natural Fungal and Bacterial Consortium from Sewage Sludge Enriched with Three Pharmaceutical Compounds
Emerging and unregulated contaminants end up in soils via stabilized/composted sewage sludges, paired with possible risks associated with the development of microbial resistance to antimicrobial agents or an imbalance in the microbial communities. An enrichment experiment was performed, fortifying t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/7/668 |
| _version_ | 1797433429200994304 |
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| author | Alejandro Ledezma-Villanueva Tatiana Robledo-Mahón Cinta Gómez-Silván Gabriela Angeles-De Paz Clementina Pozo Maximino Manzanera Concepción Calvo Elisabet Aranda |
| author_facet | Alejandro Ledezma-Villanueva Tatiana Robledo-Mahón Cinta Gómez-Silván Gabriela Angeles-De Paz Clementina Pozo Maximino Manzanera Concepción Calvo Elisabet Aranda |
| author_sort | Alejandro Ledezma-Villanueva |
| collection | DOAJ |
| description | Emerging and unregulated contaminants end up in soils via stabilized/composted sewage sludges, paired with possible risks associated with the development of microbial resistance to antimicrobial agents or an imbalance in the microbial communities. An enrichment experiment was performed, fortifying the sewage sludge with carbamazepine, ketoprofen and diclofenac as model compounds, with the aim to obtain strains with the capability to transform these pollutants. Culturable microorganisms were obtained at the end of the experiment. Among fungi, <i>Cladosporium cladosporioides</i>, <i>Alternaria alternata</i> and <i>Penicillium raistrickii</i> showed remarkable degradation rates. Population shifts in bacterial and fungal communities were also studied during the selective pressure using Illumina MiSeq. These analyses showed a predominance of Ascomycota (<i>Dothideomycetes</i> and <i>Aspergillaceae</i>) and <i>Actinobacteria</i> and <i>Proteobacteria</i>, suggesting the possibility of selecting native microorganisms to carry out bioremediation processes using tailored techniques. |
| first_indexed | 2024-03-09T10:16:54Z |
| format | Article |
| id | doaj.art-f6521c246194468fa125bfa86270e36b |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T10:16:54Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-f6521c246194468fa125bfa86270e36b2023-12-01T22:19:50ZengMDPI AGJournal of Fungi2309-608X2022-06-018766810.3390/jof8070668High-Throughput Microbial Community Analyses to Establish a Natural Fungal and Bacterial Consortium from Sewage Sludge Enriched with Three Pharmaceutical CompoundsAlejandro Ledezma-Villanueva0Tatiana Robledo-Mahón1Cinta Gómez-Silván2Gabriela Angeles-De Paz3Clementina Pozo4Maximino Manzanera5Concepción Calvo6Elisabet Aranda7Institute of Water Research, University of Granada, Ramón y Cajal, 4, 18071 Granada, SpainInstitute of Water Research, University of Granada, Ramón y Cajal, 4, 18071 Granada, SpainDepartment of Environmental Science, Policy, and Management (ESPM), University of California, Berkeley, CA 94710, USAInstitute of Water Research, University of Granada, Ramón y Cajal, 4, 18071 Granada, SpainInstitute of Water Research, University of Granada, Ramón y Cajal, 4, 18071 Granada, SpainInstitute of Water Research, University of Granada, Ramón y Cajal, 4, 18071 Granada, SpainInstitute of Water Research, University of Granada, Ramón y Cajal, 4, 18071 Granada, SpainInstitute of Water Research, University of Granada, Ramón y Cajal, 4, 18071 Granada, SpainEmerging and unregulated contaminants end up in soils via stabilized/composted sewage sludges, paired with possible risks associated with the development of microbial resistance to antimicrobial agents or an imbalance in the microbial communities. An enrichment experiment was performed, fortifying the sewage sludge with carbamazepine, ketoprofen and diclofenac as model compounds, with the aim to obtain strains with the capability to transform these pollutants. Culturable microorganisms were obtained at the end of the experiment. Among fungi, <i>Cladosporium cladosporioides</i>, <i>Alternaria alternata</i> and <i>Penicillium raistrickii</i> showed remarkable degradation rates. Population shifts in bacterial and fungal communities were also studied during the selective pressure using Illumina MiSeq. These analyses showed a predominance of Ascomycota (<i>Dothideomycetes</i> and <i>Aspergillaceae</i>) and <i>Actinobacteria</i> and <i>Proteobacteria</i>, suggesting the possibility of selecting native microorganisms to carry out bioremediation processes using tailored techniques.https://www.mdpi.com/2309-608X/8/7/668sewage sludgeemerging pollutantsselective pressuremicrobial communitiesillumina MiSeq |
| spellingShingle | Alejandro Ledezma-Villanueva Tatiana Robledo-Mahón Cinta Gómez-Silván Gabriela Angeles-De Paz Clementina Pozo Maximino Manzanera Concepción Calvo Elisabet Aranda High-Throughput Microbial Community Analyses to Establish a Natural Fungal and Bacterial Consortium from Sewage Sludge Enriched with Three Pharmaceutical Compounds Journal of Fungi sewage sludge emerging pollutants selective pressure microbial communities illumina MiSeq |
| title | High-Throughput Microbial Community Analyses to Establish a Natural Fungal and Bacterial Consortium from Sewage Sludge Enriched with Three Pharmaceutical Compounds |
| title_full | High-Throughput Microbial Community Analyses to Establish a Natural Fungal and Bacterial Consortium from Sewage Sludge Enriched with Three Pharmaceutical Compounds |
| title_fullStr | High-Throughput Microbial Community Analyses to Establish a Natural Fungal and Bacterial Consortium from Sewage Sludge Enriched with Three Pharmaceutical Compounds |
| title_full_unstemmed | High-Throughput Microbial Community Analyses to Establish a Natural Fungal and Bacterial Consortium from Sewage Sludge Enriched with Three Pharmaceutical Compounds |
| title_short | High-Throughput Microbial Community Analyses to Establish a Natural Fungal and Bacterial Consortium from Sewage Sludge Enriched with Three Pharmaceutical Compounds |
| title_sort | high throughput microbial community analyses to establish a natural fungal and bacterial consortium from sewage sludge enriched with three pharmaceutical compounds |
| topic | sewage sludge emerging pollutants selective pressure microbial communities illumina MiSeq |
| url | https://www.mdpi.com/2309-608X/8/7/668 |
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