Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment
Members of the Burkholderia cepacia complex (Bcc) are important in medical, biotechnological and agricultural disciplines. These bacteria naturally occur in soil and water environments and have adapted to survive in association with plants and animals including humans. All Bcc species are opportunis...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2011-12-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcimb.2011.00015/full |
| _version_ | 1819071019144445952 |
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| author | Eoin P O'Grady Pamela A Sokol |
| author_facet | Eoin P O'Grady Pamela A Sokol |
| author_sort | Eoin P O'Grady |
| collection | DOAJ |
| description | Members of the Burkholderia cepacia complex (Bcc) are important in medical, biotechnological and agricultural disciplines. These bacteria naturally occur in soil and water environments and have adapted to survive in association with plants and animals including humans. All Bcc species are opportunistic pathogens including Burkholderia cenocepacia that causes infections in cystic fibrosis and chronic granulomatous disease patients. The adaptation of B. cenocepacia to the host environment was assessed in a rat chronic respiratory infection model and compared to that of high cell-density in vitro-grown cultures using transcriptomics. The distribution of genes differentially expressed on chromosomes 1, 2 and 3 was relatively proportional to the size of each genomic element, whereas the proportion of plasmid-encoded genes differentially expressed was much higher relative to its size and most genes were induced in vivo. The majority of genes encoding known virulence factors, components of types II and III secretion systems and chromosome 2-encoded type IV secretion system were similarly expressed between in vitro and in vivo environments. Lower expression in vivo was detected for some genes encoding virulence factors controlled by the N¬-acyl-homoserine lactone dependent transcriptional regulator CepR and genes associated with flagellar motility, Flp type pilus formation and type VI secretion. Plasmid-encoded type IV secretion genes were markedly induced in vivo. Additional genes induced in vivo included several predicted to be involved in osmotic stress adaptation or intracellular survival, metal ion and nutrient transport, as well as those encoding outer membrane proteins. Genes identified in this study are potentially important for virulence during host-pathogen interactions and may be associated with survival and adaptation to the host environment during chronic lung infections. |
| first_indexed | 2024-12-21T17:15:10Z |
| format | Article |
| id | doaj.art-f6527d7bae4340ee94afcb8c22a550a1 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-21T17:15:10Z |
| publishDate | 2011-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-f6527d7bae4340ee94afcb8c22a550a12022-12-21T18:56:18ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882011-12-01110.3389/fcimb.2011.0001515440Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environmentEoin P O'Grady0Pamela A Sokol1University of CalgaryUniversity of CalgaryMembers of the Burkholderia cepacia complex (Bcc) are important in medical, biotechnological and agricultural disciplines. These bacteria naturally occur in soil and water environments and have adapted to survive in association with plants and animals including humans. All Bcc species are opportunistic pathogens including Burkholderia cenocepacia that causes infections in cystic fibrosis and chronic granulomatous disease patients. The adaptation of B. cenocepacia to the host environment was assessed in a rat chronic respiratory infection model and compared to that of high cell-density in vitro-grown cultures using transcriptomics. The distribution of genes differentially expressed on chromosomes 1, 2 and 3 was relatively proportional to the size of each genomic element, whereas the proportion of plasmid-encoded genes differentially expressed was much higher relative to its size and most genes were induced in vivo. The majority of genes encoding known virulence factors, components of types II and III secretion systems and chromosome 2-encoded type IV secretion system were similarly expressed between in vitro and in vivo environments. Lower expression in vivo was detected for some genes encoding virulence factors controlled by the N¬-acyl-homoserine lactone dependent transcriptional regulator CepR and genes associated with flagellar motility, Flp type pilus formation and type VI secretion. Plasmid-encoded type IV secretion genes were markedly induced in vivo. Additional genes induced in vivo included several predicted to be involved in osmotic stress adaptation or intracellular survival, metal ion and nutrient transport, as well as those encoding outer membrane proteins. Genes identified in this study are potentially important for virulence during host-pathogen interactions and may be associated with survival and adaptation to the host environment during chronic lung infections.http://journal.frontiersin.org/Journal/10.3389/fcimb.2011.00015/fullBurkholderia cenocepaciaBurkholderia cepacia complexMicroarraylung infectionrat chronic respiratory infection model |
| spellingShingle | Eoin P O'Grady Pamela A Sokol Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment Frontiers in Cellular and Infection Microbiology Burkholderia cenocepacia Burkholderia cepacia complex Microarray lung infection rat chronic respiratory infection model |
| title | Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment |
| title_full | Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment |
| title_fullStr | Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment |
| title_full_unstemmed | Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment |
| title_short | Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment |
| title_sort | burkholderia cenocepacia differential gene expression during host pathogen interactions and adaptation to the host environment |
| topic | Burkholderia cenocepacia Burkholderia cepacia complex Microarray lung infection rat chronic respiratory infection model |
| url | http://journal.frontiersin.org/Journal/10.3389/fcimb.2011.00015/full |
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