Effect of A-769662, a direct AMPK activator, on Tlr-4 expression and activity in mice heart tissue
Objective(s): TLR-4 activates a number of inflammatory signaling pathways. Also, AMPK could be involved in anti-inflammatory signaling. The aim of this study was to identify whether stimulation of AMPK could inhibit LPS-induced Tlr-4 gene expression in mice hearts. Materials and methods: Heart AMPK...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2016-12-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_7917_c6e102ce9fd836beabc0c3235560d57b.pdf |
| _version_ | 1818117084423389184 |
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| author | Maryam Rameshrad Nasrin Maleki-Dizaji Hamid Soraya Negisa Seyed Toutounchi Abolfazl Barzegari Alireza Garjani |
| author_facet | Maryam Rameshrad Nasrin Maleki-Dizaji Hamid Soraya Negisa Seyed Toutounchi Abolfazl Barzegari Alireza Garjani |
| author_sort | Maryam Rameshrad |
| collection | DOAJ |
| description | Objective(s): TLR-4 activates a number of inflammatory signaling pathways. Also, AMPK could be involved in anti-inflammatory signaling. The aim of this study was to identify whether stimulation of AMPK could inhibit LPS-induced Tlr-4 gene expression in mice hearts. Materials and methods: Heart AMPK activity and/or Tlr-4 expression was stimulated in different mice groups, using respectively IP injection of A-769662 (10 mg/kg) and LPS (2 mg/kg) or a combination of both agents. Moreover, compound-C (20 mg/kg), as an AMPK antagonist, was intraperitoneally co-administrated with both A-769662 and LPS in another group to investigate the role of AMPK activity on Tlr-4 regulation. After 8 hr, in addition to peripheral neutrophil cell count, myocardial p-AMPK, p-ACC as well as MyD88 protein contents and Tlr-4 expression was assessed by Western blotting and real-time qRT-PCR, respectively. TNF-α and IL-6 expression levels were also determined by ELISA. Results: LPS induced heart Tlr-4 expression (P |
| first_indexed | 2024-12-11T04:32:47Z |
| format | Article |
| id | doaj.art-f655effc9910457c86ac31cce8008040 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-11T04:32:47Z |
| publishDate | 2016-12-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-f655effc9910457c86ac31cce80080402022-12-22T01:20:49ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742016-12-0119121308131710.22038/ijbms.2016.79177917Effect of A-769662, a direct AMPK activator, on Tlr-4 expression and activity in mice heart tissueMaryam Rameshrad0Nasrin Maleki-Dizaji1Hamid Soraya2Negisa Seyed Toutounchi3Abolfazl Barzegari4Alireza Garjani5Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran|Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Pharmacology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, IranStudent Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranResearch Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranObjective(s): TLR-4 activates a number of inflammatory signaling pathways. Also, AMPK could be involved in anti-inflammatory signaling. The aim of this study was to identify whether stimulation of AMPK could inhibit LPS-induced Tlr-4 gene expression in mice hearts. Materials and methods: Heart AMPK activity and/or Tlr-4 expression was stimulated in different mice groups, using respectively IP injection of A-769662 (10 mg/kg) and LPS (2 mg/kg) or a combination of both agents. Moreover, compound-C (20 mg/kg), as an AMPK antagonist, was intraperitoneally co-administrated with both A-769662 and LPS in another group to investigate the role of AMPK activity on Tlr-4 regulation. After 8 hr, in addition to peripheral neutrophil cell count, myocardial p-AMPK, p-ACC as well as MyD88 protein contents and Tlr-4 expression was assessed by Western blotting and real-time qRT-PCR, respectively. TNF-α and IL-6 expression levels were also determined by ELISA. Results: LPS induced heart Tlr-4 expression (Phttp://ijbms.mums.ac.ir/article_7917_c6e102ce9fd836beabc0c3235560d57b.pdfACCA-769662AMPKCompound-C LipopolysaccharideTLR-4 |
| spellingShingle | Maryam Rameshrad Nasrin Maleki-Dizaji Hamid Soraya Negisa Seyed Toutounchi Abolfazl Barzegari Alireza Garjani Effect of A-769662, a direct AMPK activator, on Tlr-4 expression and activity in mice heart tissue Iranian Journal of Basic Medical Sciences ACC A-769662 AMPK Compound-C Lipopolysaccharide TLR-4 |
| title | Effect of A-769662, a direct AMPK activator, on Tlr-4 expression and activity in mice heart tissue |
| title_full | Effect of A-769662, a direct AMPK activator, on Tlr-4 expression and activity in mice heart tissue |
| title_fullStr | Effect of A-769662, a direct AMPK activator, on Tlr-4 expression and activity in mice heart tissue |
| title_full_unstemmed | Effect of A-769662, a direct AMPK activator, on Tlr-4 expression and activity in mice heart tissue |
| title_short | Effect of A-769662, a direct AMPK activator, on Tlr-4 expression and activity in mice heart tissue |
| title_sort | effect of a 769662 a direct ampk activator on tlr 4 expression and activity in mice heart tissue |
| topic | ACC A-769662 AMPK Compound-C Lipopolysaccharide TLR-4 |
| url | http://ijbms.mums.ac.ir/article_7917_c6e102ce9fd836beabc0c3235560d57b.pdf |
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