Cellular proteomic profiling of esophageal epithelial cells cultured under physioxia or normoxia reveals high correlation of radiation response
Objective: To investigate the radiation response and proteomic profiling of esophageal epithelial cells cultured under physioxia and normoxia. Methods: The human immortalized normal esophageal epithelial cell line SHEE cells were cultured under normoxia (21%) and physioxia (4%), respectively. A clon...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Radiation Medicine and Protection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666555723000187 |
| _version_ | 1797798640306094080 |
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| author | Hui Luo Yanan Sun Liuxiang Wang Ran Zhao Beggs James |
| author_facet | Hui Luo Yanan Sun Liuxiang Wang Ran Zhao Beggs James |
| author_sort | Hui Luo |
| collection | DOAJ |
| description | Objective: To investigate the radiation response and proteomic profiling of esophageal epithelial cells cultured under physioxia and normoxia. Methods: The human immortalized normal esophageal epithelial cell line SHEE cells were cultured under normoxia (21%) and physioxia (4%), respectively. A clonogenic assay was performed to evaluate the radiation response of SHEE cells. Cellular proteomic profiling of SHEE cells maintained under physioxia and normoxia was conducted to determine the differentially expressed proteins. Then, the identified differentially expressed proteins were validated by Western blot. Results: SHEE cells exposed to normoxia showed an increased radiation response compared to physioxia (irradiation dose ≥10 Gy, P< 0.05). Over 1200 non-redundant proteins were identified in the collected samples. Protein expression was compared between physioxia and normoxia, 42 proteins were downregulated and 45 proteins upregulated, in which oxidative phosphorylation was the most significantly enriched pathway. When cells were cultured under normoxia conditions, the induction of antioxidant genes appeared to contribute to form a phenotype adapted to the environment with high oxygen-content. Further analysis validated NRF2, BIP, VCP, SOD1, and YAP1 were the key regulators of this phenotype. Conclusions: Compared with physioxia, normoxic cell culture condition can enhance the radiation response. This study could stimulate in vivo microenvironment, and provide a basis for radiation-induced normal tissue damage. |
| first_indexed | 2024-03-13T04:06:52Z |
| format | Article |
| id | doaj.art-f65c333f1b8a4710b4cfe08702d799c0 |
| institution | Directory Open Access Journal |
| issn | 2666-5557 |
| language | English |
| last_indexed | 2024-03-13T04:06:52Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Radiation Medicine and Protection |
| spelling | doaj.art-f65c333f1b8a4710b4cfe08702d799c02023-06-21T07:00:38ZengElsevierRadiation Medicine and Protection2666-55572023-06-01428692Cellular proteomic profiling of esophageal epithelial cells cultured under physioxia or normoxia reveals high correlation of radiation responseHui Luo0Yanan Sun1Liuxiang Wang2Ran Zhao3Beggs James4Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China; Corresponding author.Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, ChinaAcademic of Medical Science, Zhengzhou University, Zhengzhou 450001, ChinaChina-US (Henan) Hormel Cancer Institute, Zhengzhou 450003, ChinaChina-US (Henan) Hormel Cancer Institute, Zhengzhou 450003, ChinaObjective: To investigate the radiation response and proteomic profiling of esophageal epithelial cells cultured under physioxia and normoxia. Methods: The human immortalized normal esophageal epithelial cell line SHEE cells were cultured under normoxia (21%) and physioxia (4%), respectively. A clonogenic assay was performed to evaluate the radiation response of SHEE cells. Cellular proteomic profiling of SHEE cells maintained under physioxia and normoxia was conducted to determine the differentially expressed proteins. Then, the identified differentially expressed proteins were validated by Western blot. Results: SHEE cells exposed to normoxia showed an increased radiation response compared to physioxia (irradiation dose ≥10 Gy, P< 0.05). Over 1200 non-redundant proteins were identified in the collected samples. Protein expression was compared between physioxia and normoxia, 42 proteins were downregulated and 45 proteins upregulated, in which oxidative phosphorylation was the most significantly enriched pathway. When cells were cultured under normoxia conditions, the induction of antioxidant genes appeared to contribute to form a phenotype adapted to the environment with high oxygen-content. Further analysis validated NRF2, BIP, VCP, SOD1, and YAP1 were the key regulators of this phenotype. Conclusions: Compared with physioxia, normoxic cell culture condition can enhance the radiation response. This study could stimulate in vivo microenvironment, and provide a basis for radiation-induced normal tissue damage.http://www.sciencedirect.com/science/article/pii/S2666555723000187Esophageal epithelial cellRadiation responseProteomicsNormoxiaPhysioxia |
| spellingShingle | Hui Luo Yanan Sun Liuxiang Wang Ran Zhao Beggs James Cellular proteomic profiling of esophageal epithelial cells cultured under physioxia or normoxia reveals high correlation of radiation response Radiation Medicine and Protection Esophageal epithelial cell Radiation response Proteomics Normoxia Physioxia |
| title | Cellular proteomic profiling of esophageal epithelial cells cultured under physioxia or normoxia reveals high correlation of radiation response |
| title_full | Cellular proteomic profiling of esophageal epithelial cells cultured under physioxia or normoxia reveals high correlation of radiation response |
| title_fullStr | Cellular proteomic profiling of esophageal epithelial cells cultured under physioxia or normoxia reveals high correlation of radiation response |
| title_full_unstemmed | Cellular proteomic profiling of esophageal epithelial cells cultured under physioxia or normoxia reveals high correlation of radiation response |
| title_short | Cellular proteomic profiling of esophageal epithelial cells cultured under physioxia or normoxia reveals high correlation of radiation response |
| title_sort | cellular proteomic profiling of esophageal epithelial cells cultured under physioxia or normoxia reveals high correlation of radiation response |
| topic | Esophageal epithelial cell Radiation response Proteomics Normoxia Physioxia |
| url | http://www.sciencedirect.com/science/article/pii/S2666555723000187 |
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