FBXO28 promotes cell proliferation, migration and invasion via upregulation of the TGF-beta1/SMAD2/3 signaling pathway in ovarian cancer
Abstract Background Ovarian cancer is one of the most common gynecological malignancies due to the lack of early symptoms, early diagnosis and limited screening. Therefore, it is necessary to understand the molecular mechanism underlying the occurrence and progression of ovarian cancer and to identi...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-01-01
|
| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-024-11893-8 |
| _version_ | 1797276514252750848 |
|---|---|
| author | Gendi Song Zhengwei Sun Man Chu Zihan Zhang Jiajia Chen Zhiwei Wang Xueqiong Zhu |
| author_facet | Gendi Song Zhengwei Sun Man Chu Zihan Zhang Jiajia Chen Zhiwei Wang Xueqiong Zhu |
| author_sort | Gendi Song |
| collection | DOAJ |
| description | Abstract Background Ovarian cancer is one of the most common gynecological malignancies due to the lack of early symptoms, early diagnosis and limited screening. Therefore, it is necessary to understand the molecular mechanism underlying the occurrence and progression of ovarian cancer and to identify a basic biomarker for the early diagnosis and clinical treatment of ovarian cancer. Methods The association between FBXO28 and ovarian cancer prognosis was analyzed using Kaplan‒Meier survival analysis. The difference in FBXO28 mRNA expression between normal ovarian tissues and ovarian tumor tissues was obtained from The Cancer Genome Atlas (TCGA), and Genotype-Tissue Expression (GTEx) cohorts. The expression levels of the FBXO28 protein in ovarian cancer tissues and normal ovarian tissues were measured via immunohistochemical staining. Western blotting was used to determine the level of FBXO28 expression in ovarian cancer cells. The CCK-8, the colony formation, Transwell migration and invasion assays were performed to evaluate cell proliferation and motility. Results We found that a higher expression level of FBXO28 was associated with poor prognosis in ovarian cancer patients. Analysis of the TCGA and GTEx cohorts showed that the FBXO28 mRNA level was lower in normal ovarian tissue samples than in ovarian cancer tissue samples. Compared with that in normal ovarian tissues or cell lines, the expression of FBXO28 was greater in ovarian tumor tissues or tumor cells. The upregulation of FBXO28 promoted the viability, proliferation, migration and invasion of ovarian cancer cells. Finally, we demonstrated that FBXO28 activated the TGF-beta1/Smad2/3 signaling pathway in ovarian cancer. Conclusions In conclusion, FBXO28 enhanced oncogenic function via upregulation of the TGF-beta1/Smad2/3 signaling pathway in ovarian cancer. |
| first_indexed | 2024-03-07T15:29:16Z |
| format | Article |
| id | doaj.art-f65c8ce514fe45cf92a0a0169fc2c140 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-03-07T15:29:16Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-f65c8ce514fe45cf92a0a0169fc2c1402024-03-05T16:32:47ZengBMCBMC Cancer1471-24072024-01-0124111310.1186/s12885-024-11893-8FBXO28 promotes cell proliferation, migration and invasion via upregulation of the TGF-beta1/SMAD2/3 signaling pathway in ovarian cancerGendi Song0Zhengwei Sun1Man Chu2Zihan Zhang3Jiajia Chen4Zhiwei Wang5Xueqiong Zhu6Zhejiang Provincial Clinical Research Center for Obstetrics and Gynecology, Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou Medical UniversityZhejiang Provincial Clinical Research Center for Obstetrics and Gynecology, Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou Medical UniversityZhejiang Provincial Clinical Research Center for Obstetrics and Gynecology, Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou Medical UniversityZhejiang Provincial Clinical Research Center for Obstetrics and Gynecology, Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou Medical UniversityZhejiang Provincial Clinical Research Center for Obstetrics and Gynecology, Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou Medical UniversityZhejiang Provincial Clinical Research Center for Obstetrics and Gynecology, Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou Medical UniversityZhejiang Provincial Clinical Research Center for Obstetrics and Gynecology, Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou Medical UniversityAbstract Background Ovarian cancer is one of the most common gynecological malignancies due to the lack of early symptoms, early diagnosis and limited screening. Therefore, it is necessary to understand the molecular mechanism underlying the occurrence and progression of ovarian cancer and to identify a basic biomarker for the early diagnosis and clinical treatment of ovarian cancer. Methods The association between FBXO28 and ovarian cancer prognosis was analyzed using Kaplan‒Meier survival analysis. The difference in FBXO28 mRNA expression between normal ovarian tissues and ovarian tumor tissues was obtained from The Cancer Genome Atlas (TCGA), and Genotype-Tissue Expression (GTEx) cohorts. The expression levels of the FBXO28 protein in ovarian cancer tissues and normal ovarian tissues were measured via immunohistochemical staining. Western blotting was used to determine the level of FBXO28 expression in ovarian cancer cells. The CCK-8, the colony formation, Transwell migration and invasion assays were performed to evaluate cell proliferation and motility. Results We found that a higher expression level of FBXO28 was associated with poor prognosis in ovarian cancer patients. Analysis of the TCGA and GTEx cohorts showed that the FBXO28 mRNA level was lower in normal ovarian tissue samples than in ovarian cancer tissue samples. Compared with that in normal ovarian tissues or cell lines, the expression of FBXO28 was greater in ovarian tumor tissues or tumor cells. The upregulation of FBXO28 promoted the viability, proliferation, migration and invasion of ovarian cancer cells. Finally, we demonstrated that FBXO28 activated the TGF-beta1/Smad2/3 signaling pathway in ovarian cancer. Conclusions In conclusion, FBXO28 enhanced oncogenic function via upregulation of the TGF-beta1/Smad2/3 signaling pathway in ovarian cancer.https://doi.org/10.1186/s12885-024-11893-8FBXO28TGF-b1Ovarian cancerSmad2/3Migration |
| spellingShingle | Gendi Song Zhengwei Sun Man Chu Zihan Zhang Jiajia Chen Zhiwei Wang Xueqiong Zhu FBXO28 promotes cell proliferation, migration and invasion via upregulation of the TGF-beta1/SMAD2/3 signaling pathway in ovarian cancer BMC Cancer FBXO28 TGF-b1 Ovarian cancer Smad2/3 Migration |
| title | FBXO28 promotes cell proliferation, migration and invasion via upregulation of the TGF-beta1/SMAD2/3 signaling pathway in ovarian cancer |
| title_full | FBXO28 promotes cell proliferation, migration and invasion via upregulation of the TGF-beta1/SMAD2/3 signaling pathway in ovarian cancer |
| title_fullStr | FBXO28 promotes cell proliferation, migration and invasion via upregulation of the TGF-beta1/SMAD2/3 signaling pathway in ovarian cancer |
| title_full_unstemmed | FBXO28 promotes cell proliferation, migration and invasion via upregulation of the TGF-beta1/SMAD2/3 signaling pathway in ovarian cancer |
| title_short | FBXO28 promotes cell proliferation, migration and invasion via upregulation of the TGF-beta1/SMAD2/3 signaling pathway in ovarian cancer |
| title_sort | fbxo28 promotes cell proliferation migration and invasion via upregulation of the tgf beta1 smad2 3 signaling pathway in ovarian cancer |
| topic | FBXO28 TGF-b1 Ovarian cancer Smad2/3 Migration |
| url | https://doi.org/10.1186/s12885-024-11893-8 |
| work_keys_str_mv | AT gendisong fbxo28promotescellproliferationmigrationandinvasionviaupregulationofthetgfbeta1smad23signalingpathwayinovariancancer AT zhengweisun fbxo28promotescellproliferationmigrationandinvasionviaupregulationofthetgfbeta1smad23signalingpathwayinovariancancer AT manchu fbxo28promotescellproliferationmigrationandinvasionviaupregulationofthetgfbeta1smad23signalingpathwayinovariancancer AT zihanzhang fbxo28promotescellproliferationmigrationandinvasionviaupregulationofthetgfbeta1smad23signalingpathwayinovariancancer AT jiajiachen fbxo28promotescellproliferationmigrationandinvasionviaupregulationofthetgfbeta1smad23signalingpathwayinovariancancer AT zhiweiwang fbxo28promotescellproliferationmigrationandinvasionviaupregulationofthetgfbeta1smad23signalingpathwayinovariancancer AT xueqiongzhu fbxo28promotescellproliferationmigrationandinvasionviaupregulationofthetgfbeta1smad23signalingpathwayinovariancancer |