In vivo labeling and quantitative imaging of neuronal populations using MRI
The study of neural circuits, which underlies perception, cognition, emotion, and behavior, is essential for understanding the mammalian brain, a complex organ consisting of billions of neurons. To study the structure and function of the brain, in vivo neuronal labeling and imaging techniques are cr...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-11-01
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| Series: | NeuroImage |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1053811923005256 |
| _version_ | 1797663911399391232 |
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| author | Shana Li Xiang Xu Canjun Li Ziyan Xu Ke Wu Qiong Ye Yan Zhang Xiaohua Jiang Chunlei Cang Changlin Tian Jie Wen |
| author_facet | Shana Li Xiang Xu Canjun Li Ziyan Xu Ke Wu Qiong Ye Yan Zhang Xiaohua Jiang Chunlei Cang Changlin Tian Jie Wen |
| author_sort | Shana Li |
| collection | DOAJ |
| description | The study of neural circuits, which underlies perception, cognition, emotion, and behavior, is essential for understanding the mammalian brain, a complex organ consisting of billions of neurons. To study the structure and function of the brain, in vivo neuronal labeling and imaging techniques are crucial as they provide true physiological information that ex vivo methods cannot offer. In this paper, we present a new strategy for in vivo neuronal labeling and quantification using MRI. We demonstrate the efficacy of this method by delivering the oatp1a1 gene to the target neurons using rAAV2-retro virus. OATP1A1 protein expression on the neuronal membrane increased the uptake of a specific MRI contrast agent (Gd-EOB-DTPA), leading to hyperintense signals on T1W images of labeled neuronal populations. We also used dynamic contrast enhancement-based methods to obtain quantitative information on labeled neuronal populations in vivo. |
| first_indexed | 2024-03-11T19:22:36Z |
| format | Article |
| id | doaj.art-f65f132889764146be997ee618cfcbda |
| institution | Directory Open Access Journal |
| issn | 1095-9572 |
| language | English |
| last_indexed | 2024-03-11T19:22:36Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | NeuroImage |
| spelling | doaj.art-f65f132889764146be997ee618cfcbda2023-10-07T04:33:50ZengElsevierNeuroImage1095-95722023-11-01281120374In vivo labeling and quantitative imaging of neuronal populations using MRIShana Li0Xiang Xu1Canjun Li2Ziyan Xu3Ke Wu4Qiong Ye5Yan Zhang6Xiaohua Jiang7Chunlei Cang8Changlin Tian9Jie Wen10The First Affiliated Hospital of USTC (Anhui Provincial Hospital), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, PR ChinaThe First Affiliated Hospital of USTC (Anhui Provincial Hospital), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, PR ChinaSchool of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, PR ChinaSchool of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, PR ChinaThe First Affiliated Hospital of USTC (Anhui Provincial Hospital), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, PR ChinaKey Laboratory of High Field Magnetic Resonance Image of Anhui Province, High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, PR ChinaThe First Affiliated Hospital of USTC (Anhui Provincial Hospital), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, PR ChinaThe First Affiliated Hospital of USTC (Anhui Provincial Hospital), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, PR ChinaSchool of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, PR ChinaThe First Affiliated Hospital of USTC (Anhui Provincial Hospital), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, PR China; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, PR China; Key Laboratory of High Field Magnetic Resonance Image of Anhui Province, High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, PR China; Co-corresponding author.The First Affiliated Hospital of USTC (Anhui Provincial Hospital), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, PR China; Corresponding author.The study of neural circuits, which underlies perception, cognition, emotion, and behavior, is essential for understanding the mammalian brain, a complex organ consisting of billions of neurons. To study the structure and function of the brain, in vivo neuronal labeling and imaging techniques are crucial as they provide true physiological information that ex vivo methods cannot offer. In this paper, we present a new strategy for in vivo neuronal labeling and quantification using MRI. We demonstrate the efficacy of this method by delivering the oatp1a1 gene to the target neurons using rAAV2-retro virus. OATP1A1 protein expression on the neuronal membrane increased the uptake of a specific MRI contrast agent (Gd-EOB-DTPA), leading to hyperintense signals on T1W images of labeled neuronal populations. We also used dynamic contrast enhancement-based methods to obtain quantitative information on labeled neuronal populations in vivo.http://www.sciencedirect.com/science/article/pii/S1053811923005256Reporter geneIn vivo MRINeuronal labelingOatp1a1Gd-EOB-DTPAQuantitative analysis |
| spellingShingle | Shana Li Xiang Xu Canjun Li Ziyan Xu Ke Wu Qiong Ye Yan Zhang Xiaohua Jiang Chunlei Cang Changlin Tian Jie Wen In vivo labeling and quantitative imaging of neuronal populations using MRI NeuroImage Reporter gene In vivo MRI Neuronal labeling Oatp1a1 Gd-EOB-DTPA Quantitative analysis |
| title | In vivo labeling and quantitative imaging of neuronal populations using MRI |
| title_full | In vivo labeling and quantitative imaging of neuronal populations using MRI |
| title_fullStr | In vivo labeling and quantitative imaging of neuronal populations using MRI |
| title_full_unstemmed | In vivo labeling and quantitative imaging of neuronal populations using MRI |
| title_short | In vivo labeling and quantitative imaging of neuronal populations using MRI |
| title_sort | in vivo labeling and quantitative imaging of neuronal populations using mri |
| topic | Reporter gene In vivo MRI Neuronal labeling Oatp1a1 Gd-EOB-DTPA Quantitative analysis |
| url | http://www.sciencedirect.com/science/article/pii/S1053811923005256 |
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