Identification of RT-qPCR reference genes suitable for gene function studies in the pitaya canker disease pathogen Neoscytalidium dimidiatum
Abstract Neoscytalidium dimidiatum is the main causal agent of pitaya canker. Most studies of virulence and pathogenicity genes have measured expression levels using real-time quantitative polymerase chain reaction (RT-qPCR). Suitable reference genes are essential for ensuring that estimates of gene...
Main Authors: | Meng Wang, Zhouwen Wang, Shuangshuang Wei, Jun Xie, Jiaquan Huang, Dongdong Li, Wenbin Hu, Hongli Li, Hua Tang |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2022-12-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-022-27041-w |
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