Genome-Wide Identification and Transcriptional Analysis of Arabidopsis <i>DUF506</i> Gene Family
The Domain of unknown function 506 (<i>DUF506</i>) family, which belongs to the PD-(D/E)XK nuclease superfamily, has not been functionally characterized. In this study, 266 <i>DUF506</i> domain-containing genes were identified from algae, mosses, and land plants showing their...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/21/11442 |
| Summary: | The Domain of unknown function 506 (<i>DUF506</i>) family, which belongs to the PD-(D/E)XK nuclease superfamily, has not been functionally characterized. In this study, 266 <i>DUF506</i> domain-containing genes were identified from algae, mosses, and land plants showing their wide occurrence in photosynthetic organisms. Bioinformatics analysis identified 211 high-confidence <i>DUF506</i> genes across 17 representative land plant species. Phylogenetic modeling classified three groups of plant <i>DUF506</i> genes that suggested functional preservation among the groups based on conserved gene structure and motifs. Gene duplication and Ka/Ks evolutionary rates revealed that <i>DUF506</i> genes are under purifying positive selection pressure. Subcellular protein localization analysis revealed that <i>DUF506</i> proteins were present in different organelles. Transcript analyses showed that 13 of the Arabidopsis <i>DUF506</i> genes are ubiquitously expressed in various tissues and respond to different abiotic stresses and ABA treatment. Protein-protein interaction network analysis using the STRING-DB, AtPIN (<i>Arabidopsis thaliana</i> Protein Interaction Network), and AI-1 (Arabidopsis Interactome-1) tools indicated that AtDUF506s potentially interact with iron-deficiency response proteins, salt-inducible transcription factors, or calcium sensors (calmodulins), implying that <i>DUF506</i> genes have distinct biological functions including responses to environmental stimuli, nutrient-deficiencies, and participate in Ca(2+) signaling. Current results provide insightful information regarding the molecular features of the <i>DUF506</i> family in plants, to support further functional characterizations. |
|---|---|
| ISSN: | 1661-6596 1422-0067 |