Genome-Wide Identification and Expression Analysis of the Stearoyl-Acyl Carrier Protein Δ9 Desaturase Gene Family under Abiotic Stress in Barley
Stearoyl-acyl carrier protein (ACP) Δ9 desaturase (SAD) is a critical fatty acid dehydrogenase in plants, playing a prominent role in regulating the synthesis of unsaturated fatty acids (UFAs) and having a significant impact on plant growth and development. In this study, we conducted a comprehensiv...
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/1/113 |
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| author | Mingyu Ding Danni Zhou Yichen Ye Shuting Wen Xian Zhang Quanxiang Tian Xiaoqin Zhang Wangshu Mou Cong Dang Yunxia Fang Dawei Xue |
| author_facet | Mingyu Ding Danni Zhou Yichen Ye Shuting Wen Xian Zhang Quanxiang Tian Xiaoqin Zhang Wangshu Mou Cong Dang Yunxia Fang Dawei Xue |
| author_sort | Mingyu Ding |
| collection | DOAJ |
| description | Stearoyl-acyl carrier protein (ACP) Δ9 desaturase (SAD) is a critical fatty acid dehydrogenase in plants, playing a prominent role in regulating the synthesis of unsaturated fatty acids (UFAs) and having a significant impact on plant growth and development. In this study, we conducted a comprehensive genomic analysis of the SAD family in barley (<i>Hordeum vulgare</i> L.), identifying 14 HvSADs with the FA_desaturase_2 domain, which were divided into four subgroups based on sequence composition and phylogenetic analysis, with members of the same subgroup possessing similar genes and motif structures. Gene replication analysis suggested that tandem and segmental duplication may be the major reasons for the expansion of the <i>SAD</i> family in barley. The promoters of <i>HvSADs</i> contained various <i>cis</i>-regulatory elements (CREs) related to light, abscisic acid (ABA), and methyl jasmonate (MeJA). In addition, expression analysis indicated that <i>HvSADs</i> exhibit multiple tissue expression patterns in barley as well as different response characteristics under three abiotic stresses: salt, drought, and cold. Briefly, this evolutionary and expression analysis of <i>HvSADs</i> provides insight into the biological functions of barley, supporting a comprehensive analysis of the regulatory mechanisms of oil biosynthesis and metabolism in plants under abiotic stress. |
| first_indexed | 2024-03-08T15:06:55Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T15:06:55Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f66b25839cf14eb18902c6da4a1073992024-01-10T14:58:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0125111310.3390/ijms25010113Genome-Wide Identification and Expression Analysis of the Stearoyl-Acyl Carrier Protein Δ9 Desaturase Gene Family under Abiotic Stress in BarleyMingyu Ding0Danni Zhou1Yichen Ye2Shuting Wen3Xian Zhang4Quanxiang Tian5Xiaoqin Zhang6Wangshu Mou7Cong Dang8Yunxia Fang9Dawei Xue10College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaStearoyl-acyl carrier protein (ACP) Δ9 desaturase (SAD) is a critical fatty acid dehydrogenase in plants, playing a prominent role in regulating the synthesis of unsaturated fatty acids (UFAs) and having a significant impact on plant growth and development. In this study, we conducted a comprehensive genomic analysis of the SAD family in barley (<i>Hordeum vulgare</i> L.), identifying 14 HvSADs with the FA_desaturase_2 domain, which were divided into four subgroups based on sequence composition and phylogenetic analysis, with members of the same subgroup possessing similar genes and motif structures. Gene replication analysis suggested that tandem and segmental duplication may be the major reasons for the expansion of the <i>SAD</i> family in barley. The promoters of <i>HvSADs</i> contained various <i>cis</i>-regulatory elements (CREs) related to light, abscisic acid (ABA), and methyl jasmonate (MeJA). In addition, expression analysis indicated that <i>HvSADs</i> exhibit multiple tissue expression patterns in barley as well as different response characteristics under three abiotic stresses: salt, drought, and cold. Briefly, this evolutionary and expression analysis of <i>HvSADs</i> provides insight into the biological functions of barley, supporting a comprehensive analysis of the regulatory mechanisms of oil biosynthesis and metabolism in plants under abiotic stress.https://www.mdpi.com/1422-0067/25/1/113barleySADevolutionunsaturated fatty acids (UFAs)abiotic stress |
| spellingShingle | Mingyu Ding Danni Zhou Yichen Ye Shuting Wen Xian Zhang Quanxiang Tian Xiaoqin Zhang Wangshu Mou Cong Dang Yunxia Fang Dawei Xue Genome-Wide Identification and Expression Analysis of the Stearoyl-Acyl Carrier Protein Δ9 Desaturase Gene Family under Abiotic Stress in Barley International Journal of Molecular Sciences barley SAD evolution unsaturated fatty acids (UFAs) abiotic stress |
| title | Genome-Wide Identification and Expression Analysis of the Stearoyl-Acyl Carrier Protein Δ9 Desaturase Gene Family under Abiotic Stress in Barley |
| title_full | Genome-Wide Identification and Expression Analysis of the Stearoyl-Acyl Carrier Protein Δ9 Desaturase Gene Family under Abiotic Stress in Barley |
| title_fullStr | Genome-Wide Identification and Expression Analysis of the Stearoyl-Acyl Carrier Protein Δ9 Desaturase Gene Family under Abiotic Stress in Barley |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of the Stearoyl-Acyl Carrier Protein Δ9 Desaturase Gene Family under Abiotic Stress in Barley |
| title_short | Genome-Wide Identification and Expression Analysis of the Stearoyl-Acyl Carrier Protein Δ9 Desaturase Gene Family under Abiotic Stress in Barley |
| title_sort | genome wide identification and expression analysis of the stearoyl acyl carrier protein δ9 desaturase gene family under abiotic stress in barley |
| topic | barley SAD evolution unsaturated fatty acids (UFAs) abiotic stress |
| url | https://www.mdpi.com/1422-0067/25/1/113 |
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