Hemostatic capability of ultrafiltrated fresh frozen plasma compared to cryoprecipitate
Abstract This in vitro study evaluated the potential hemostatic effect of fresh frozen plasma (FFP) ultrafiltration on clotting factors, coagulation parameters, and plasma properties. ABO-specific units of FFP (n = 40) were prepared for the concentrated FFP and cryoprecipitate. Plasma water was remo...
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| Format: | Article |
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Nature Portfolio
2023-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-48759-1 |
| _version_ | 1827590670898954240 |
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| author | Junko Ichikawa Toshiaki Iba Ryouta Okazaki Tomoki Fukuda Mitsuharu Kodaka Makiko Komori Jerrold H. Levy |
| author_facet | Junko Ichikawa Toshiaki Iba Ryouta Okazaki Tomoki Fukuda Mitsuharu Kodaka Makiko Komori Jerrold H. Levy |
| author_sort | Junko Ichikawa |
| collection | DOAJ |
| description | Abstract This in vitro study evaluated the potential hemostatic effect of fresh frozen plasma (FFP) ultrafiltration on clotting factors, coagulation parameters, and plasma properties. ABO-specific units of FFP (n = 40) were prepared for the concentrated FFP and cryoprecipitate. Plasma water was removed from FFP by ultrafiltration using a dialyzer with a pump running at a 300 mL/min. The aliquot of each concentrated FFP after 50, 100, 200, and 250 mL of fluid removal were measured the standard coagulation assay, clotting activity, and plasma properties to compare those parameters of cryoprecipitate. Concentrated FFP contained 36.5% of fibrinogen in FFP with a mean concentration of 7.2 g/L, lower than the cryoprecipitate level. The levels of factor VIII (FVIII), von Willebrand factor (VWF):antigen (Ag), and VWF:ristocetin cofactor (RCo) were also lower in concentrated FFP, whereas the levels of factor V, factor IX, factor XIII, antithrombin and albumin was higher in concentrated FFP. Maximum clot firmness (MCF) in thromboelastometry was approximately one-half of that in cryoprecipitate. Although the levels of VWF:Ag, VWF:RCo, and FVIII differed depending on the ABO blood types, fibrinogen levels, and MCF were not significantly different among the ABO blood groups in FFP and concentrated FFP. |
| first_indexed | 2024-03-09T01:20:05Z |
| format | Article |
| id | doaj.art-f66fc758a7944dd1bc7b72397bbab80f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T01:20:05Z |
| publishDate | 2023-12-01 |
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| series | Scientific Reports |
| spelling | doaj.art-f66fc758a7944dd1bc7b72397bbab80f2023-12-10T12:14:44ZengNature PortfolioScientific Reports2045-23222023-12-011311810.1038/s41598-023-48759-1Hemostatic capability of ultrafiltrated fresh frozen plasma compared to cryoprecipitateJunko Ichikawa0Toshiaki Iba1Ryouta Okazaki2Tomoki Fukuda3Mitsuharu Kodaka4Makiko Komori5Jerrold H. Levy6Department of Anesthesiology, Tokyo Women’s Medical University, Adachi Medical CenterDepartment of Emergency and Disaster Medicine, Juntendo University Graduate School of MedicineDepartment of Anesthesiology, Tokyo Women’s Medical University, Adachi Medical CenterDepartment of Anesthesiology, Tokyo Women’s Medical University, Adachi Medical CenterDepartment of Anesthesiology, Tokyo Women’s Medical University, Adachi Medical CenterDepartment of Anesthesiology, Tokyo Women’s Medical University, Adachi Medical CenterDepartment of Anesthesiology, Critical Care, and Surgery, Duke University School of MedicineAbstract This in vitro study evaluated the potential hemostatic effect of fresh frozen plasma (FFP) ultrafiltration on clotting factors, coagulation parameters, and plasma properties. ABO-specific units of FFP (n = 40) were prepared for the concentrated FFP and cryoprecipitate. Plasma water was removed from FFP by ultrafiltration using a dialyzer with a pump running at a 300 mL/min. The aliquot of each concentrated FFP after 50, 100, 200, and 250 mL of fluid removal were measured the standard coagulation assay, clotting activity, and plasma properties to compare those parameters of cryoprecipitate. Concentrated FFP contained 36.5% of fibrinogen in FFP with a mean concentration of 7.2 g/L, lower than the cryoprecipitate level. The levels of factor VIII (FVIII), von Willebrand factor (VWF):antigen (Ag), and VWF:ristocetin cofactor (RCo) were also lower in concentrated FFP, whereas the levels of factor V, factor IX, factor XIII, antithrombin and albumin was higher in concentrated FFP. Maximum clot firmness (MCF) in thromboelastometry was approximately one-half of that in cryoprecipitate. Although the levels of VWF:Ag, VWF:RCo, and FVIII differed depending on the ABO blood types, fibrinogen levels, and MCF were not significantly different among the ABO blood groups in FFP and concentrated FFP.https://doi.org/10.1038/s41598-023-48759-1 |
| spellingShingle | Junko Ichikawa Toshiaki Iba Ryouta Okazaki Tomoki Fukuda Mitsuharu Kodaka Makiko Komori Jerrold H. Levy Hemostatic capability of ultrafiltrated fresh frozen plasma compared to cryoprecipitate Scientific Reports |
| title | Hemostatic capability of ultrafiltrated fresh frozen plasma compared to cryoprecipitate |
| title_full | Hemostatic capability of ultrafiltrated fresh frozen plasma compared to cryoprecipitate |
| title_fullStr | Hemostatic capability of ultrafiltrated fresh frozen plasma compared to cryoprecipitate |
| title_full_unstemmed | Hemostatic capability of ultrafiltrated fresh frozen plasma compared to cryoprecipitate |
| title_short | Hemostatic capability of ultrafiltrated fresh frozen plasma compared to cryoprecipitate |
| title_sort | hemostatic capability of ultrafiltrated fresh frozen plasma compared to cryoprecipitate |
| url | https://doi.org/10.1038/s41598-023-48759-1 |
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