<i>Astragalus</i> Shiitake—A Novel Functional Food with High Polysaccharide Content and Anti-Proliferative Activity in a Colorectal Carcinoma Cell Line
The chemical and nutritional constituents of mushrooms can alter significantly when grown on different substrates. Based on this fact, an approach was made to cultivate a new type of mushroom, Hengshan <i>Astragalus</i> Shiitake, by growing Shiitake mushrooms on beds supplemented with th...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/11/2333 |
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| author | Bunu Tamang Qi Liang Biju Balakrishnan Su Peng Wei Zhang |
| author_facet | Bunu Tamang Qi Liang Biju Balakrishnan Su Peng Wei Zhang |
| author_sort | Bunu Tamang |
| collection | DOAJ |
| description | The chemical and nutritional constituents of mushrooms can alter significantly when grown on different substrates. Based on this fact, an approach was made to cultivate a new type of mushroom, Hengshan <i>Astragalus</i> Shiitake, by growing Shiitake mushrooms on beds supplemented with the roots of an edible herbal plant, <i>Astragalus membranaceus</i>. In this study, three green extraction techniques, including microwave-enzyme assisted (MEA), ultrasound-enzyme assisted (UEA) and microwave-ultrasound-enzyme assisted (MUEA) extractions, were used to compare both the yield and antiproliferative activity of the polysaccharide-rich extracts (PREs) from HAS in human colorectal carcinoma cells (HCT 116). Both HAS-A and HAS-B extracts contain significantly higher amounts of polysaccharides when compared to the control (Shiitake extract), regardless of the extraction methods. The PREs from HAS-B have significantly higher anti-proliferative activity in HCT 116 compared to the control when using the UEA extraction method. Our findings demonstrate that HAS-B can become a novel functional food with anti-proliferative activities and the optimization of UEA extraction would help to develop new active extract-based health products. |
| first_indexed | 2024-03-10T01:00:11Z |
| format | Article |
| id | doaj.art-f6707d65fc584bd88828304bfcbb9de2 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T01:00:11Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-f6707d65fc584bd88828304bfcbb9de22023-11-23T14:37:21ZengMDPI AGNutrients2072-66432022-06-011411233310.3390/nu14112333<i>Astragalus</i> Shiitake—A Novel Functional Food with High Polysaccharide Content and Anti-Proliferative Activity in a Colorectal Carcinoma Cell LineBunu Tamang0Qi Liang1Biju Balakrishnan2Su Peng3Wei Zhang4Advanced Marine Biomanufacturing Laboratory, Centre for Marine Bioproducts Development, College of Medicine and Public Health, Flinders University, Bedford Park, SA 5042, AustraliaAdvanced Marine Biomanufacturing Laboratory, Centre for Marine Bioproducts Development, College of Medicine and Public Health, Flinders University, Bedford Park, SA 5042, AustraliaAdvanced Marine Biomanufacturing Laboratory, Centre for Marine Bioproducts Development, College of Medicine and Public Health, Flinders University, Bedford Park, SA 5042, AustraliaAdvanced Marine Biomanufacturing Laboratory, Centre for Marine Bioproducts Development, College of Medicine and Public Health, Flinders University, Bedford Park, SA 5042, AustraliaAdvanced Marine Biomanufacturing Laboratory, Centre for Marine Bioproducts Development, College of Medicine and Public Health, Flinders University, Bedford Park, SA 5042, AustraliaThe chemical and nutritional constituents of mushrooms can alter significantly when grown on different substrates. Based on this fact, an approach was made to cultivate a new type of mushroom, Hengshan <i>Astragalus</i> Shiitake, by growing Shiitake mushrooms on beds supplemented with the roots of an edible herbal plant, <i>Astragalus membranaceus</i>. In this study, three green extraction techniques, including microwave-enzyme assisted (MEA), ultrasound-enzyme assisted (UEA) and microwave-ultrasound-enzyme assisted (MUEA) extractions, were used to compare both the yield and antiproliferative activity of the polysaccharide-rich extracts (PREs) from HAS in human colorectal carcinoma cells (HCT 116). Both HAS-A and HAS-B extracts contain significantly higher amounts of polysaccharides when compared to the control (Shiitake extract), regardless of the extraction methods. The PREs from HAS-B have significantly higher anti-proliferative activity in HCT 116 compared to the control when using the UEA extraction method. Our findings demonstrate that HAS-B can become a novel functional food with anti-proliferative activities and the optimization of UEA extraction would help to develop new active extract-based health products.https://www.mdpi.com/2072-6643/14/11/2333shiitake mushroom<i>Astragalus membranaceus</i>hengshan <i>Astragalus</i> shiitake (HAS)polysaccharidesbiotransformationanti-proliferative activity |
| spellingShingle | Bunu Tamang Qi Liang Biju Balakrishnan Su Peng Wei Zhang <i>Astragalus</i> Shiitake—A Novel Functional Food with High Polysaccharide Content and Anti-Proliferative Activity in a Colorectal Carcinoma Cell Line Nutrients shiitake mushroom <i>Astragalus membranaceus</i> hengshan <i>Astragalus</i> shiitake (HAS) polysaccharides biotransformation anti-proliferative activity |
| title | <i>Astragalus</i> Shiitake—A Novel Functional Food with High Polysaccharide Content and Anti-Proliferative Activity in a Colorectal Carcinoma Cell Line |
| title_full | <i>Astragalus</i> Shiitake—A Novel Functional Food with High Polysaccharide Content and Anti-Proliferative Activity in a Colorectal Carcinoma Cell Line |
| title_fullStr | <i>Astragalus</i> Shiitake—A Novel Functional Food with High Polysaccharide Content and Anti-Proliferative Activity in a Colorectal Carcinoma Cell Line |
| title_full_unstemmed | <i>Astragalus</i> Shiitake—A Novel Functional Food with High Polysaccharide Content and Anti-Proliferative Activity in a Colorectal Carcinoma Cell Line |
| title_short | <i>Astragalus</i> Shiitake—A Novel Functional Food with High Polysaccharide Content and Anti-Proliferative Activity in a Colorectal Carcinoma Cell Line |
| title_sort | i astragalus i shiitake a novel functional food with high polysaccharide content and anti proliferative activity in a colorectal carcinoma cell line |
| topic | shiitake mushroom <i>Astragalus membranaceus</i> hengshan <i>Astragalus</i> shiitake (HAS) polysaccharides biotransformation anti-proliferative activity |
| url | https://www.mdpi.com/2072-6643/14/11/2333 |
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