Improving Diagnosis of Cervical Pre-Cancer: Combination of PCA and SVM Applied on Fluorescence Lifetime Images
We report a significant improvement in the diagnosis of cervical cancer through a combined application of principal component analysis (PCA) and support vector machine (SVM) on the average fluorescence decay profile of Fluorescence Lifetime Images (FLI) of epithelial hyperplasia (EH) and CIN-I cervi...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-12-01
|
| Series: | Photonics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-6732/5/4/57 |
| _version_ | 1831806073075400704 |
|---|---|
| author | Gyana Ranjan Sahoo Pankaj Singh Kiran Pandey Chayanika Kala Asima Pradhan |
| author_facet | Gyana Ranjan Sahoo Pankaj Singh Kiran Pandey Chayanika Kala Asima Pradhan |
| author_sort | Gyana Ranjan Sahoo |
| collection | DOAJ |
| description | We report a significant improvement in the diagnosis of cervical cancer through a combined application of principal component analysis (PCA) and support vector machine (SVM) on the average fluorescence decay profile of Fluorescence Lifetime Images (FLI) of epithelial hyperplasia (EH) and CIN-I cervical tissue samples, obtained ex-vivo. The fast and slow components of double exponential fitted fluorescence lifetimes were found to be higher for EH compared to the lifetimes of CIN-I samples. Application of PCA to the average time-resolved fluorescence decay profiles showed that the 2nd PC, in combination with 1st PC, enhanced the discrimination between EH and CIN-I tissues. Fluorescence lifetime and PC scores were then classified separately by using SVM support vector machine to identify the two. On applying SVM to a combination of fluorescence lifetime and PC scores, diagnostic capability improved significantly. |
| first_indexed | 2024-12-22T19:35:46Z |
| format | Article |
| id | doaj.art-f672d8d8c6e441d2a8c4e7896e081942 |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-12-22T19:35:46Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-f672d8d8c6e441d2a8c4e7896e0819422022-12-21T18:14:59ZengMDPI AGPhotonics2304-67322018-12-01545710.3390/photonics5040057photonics5040057Improving Diagnosis of Cervical Pre-Cancer: Combination of PCA and SVM Applied on Fluorescence Lifetime ImagesGyana Ranjan Sahoo0Pankaj Singh1Kiran Pandey2Chayanika Kala3Asima Pradhan4Department of Physics, IIT Kanpur, Kanpur 208016, IndiaDepartment of Physics, IIT Kanpur, Kanpur 208016, IndiaDepartment of Obstetrics & Gynaecology, GSVM Medical College Kanpur, Kanpur 208002, IndiaDepartment of Pathology, LPS Institute of Cadiology, Kanpur 208019, IndiaDepartment of Physics, IIT Kanpur, Kanpur 208016, IndiaWe report a significant improvement in the diagnosis of cervical cancer through a combined application of principal component analysis (PCA) and support vector machine (SVM) on the average fluorescence decay profile of Fluorescence Lifetime Images (FLI) of epithelial hyperplasia (EH) and CIN-I cervical tissue samples, obtained ex-vivo. The fast and slow components of double exponential fitted fluorescence lifetimes were found to be higher for EH compared to the lifetimes of CIN-I samples. Application of PCA to the average time-resolved fluorescence decay profiles showed that the 2nd PC, in combination with 1st PC, enhanced the discrimination between EH and CIN-I tissues. Fluorescence lifetime and PC scores were then classified separately by using SVM support vector machine to identify the two. On applying SVM to a combination of fluorescence lifetime and PC scores, diagnostic capability improved significantly.https://www.mdpi.com/2304-6732/5/4/57fluorescence lifetimePCAPC scoresSVM |
| spellingShingle | Gyana Ranjan Sahoo Pankaj Singh Kiran Pandey Chayanika Kala Asima Pradhan Improving Diagnosis of Cervical Pre-Cancer: Combination of PCA and SVM Applied on Fluorescence Lifetime Images Photonics fluorescence lifetime PCA PC scores SVM |
| title | Improving Diagnosis of Cervical Pre-Cancer: Combination of PCA and SVM Applied on Fluorescence Lifetime Images |
| title_full | Improving Diagnosis of Cervical Pre-Cancer: Combination of PCA and SVM Applied on Fluorescence Lifetime Images |
| title_fullStr | Improving Diagnosis of Cervical Pre-Cancer: Combination of PCA and SVM Applied on Fluorescence Lifetime Images |
| title_full_unstemmed | Improving Diagnosis of Cervical Pre-Cancer: Combination of PCA and SVM Applied on Fluorescence Lifetime Images |
| title_short | Improving Diagnosis of Cervical Pre-Cancer: Combination of PCA and SVM Applied on Fluorescence Lifetime Images |
| title_sort | improving diagnosis of cervical pre cancer combination of pca and svm applied on fluorescence lifetime images |
| topic | fluorescence lifetime PCA PC scores SVM |
| url | https://www.mdpi.com/2304-6732/5/4/57 |
| work_keys_str_mv | AT gyanaranjansahoo improvingdiagnosisofcervicalprecancercombinationofpcaandsvmappliedonfluorescencelifetimeimages AT pankajsingh improvingdiagnosisofcervicalprecancercombinationofpcaandsvmappliedonfluorescencelifetimeimages AT kiranpandey improvingdiagnosisofcervicalprecancercombinationofpcaandsvmappliedonfluorescencelifetimeimages AT chayanikakala improvingdiagnosisofcervicalprecancercombinationofpcaandsvmappliedonfluorescencelifetimeimages AT asimapradhan improvingdiagnosisofcervicalprecancercombinationofpcaandsvmappliedonfluorescencelifetimeimages |