Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair
Femoral shaft fracture is a common bone trauma in dogs. The limitation of mesenchymal stem cells in bone defect applications is that the cell suspension cannot be fixed to the bone defect site. In the study, our objective was to substantiate the combined application of canine bone marrow mesenchymal...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1162407/full |
| _version_ | 1797798507976851456 |
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| author | Zihang Ma Zihang Ma Xiaoying Guo Xiaoying Guo Jun Zhang Jun Zhang Jun Zhang Qifeng Jiang Qifeng Jiang Wuying Liang Wuying Liang Wenxin Meng Wenxin Meng Shuaijiang Chen Shuaijiang Chen Yufan Zhu Yufan Zhu Cundong Ye Cundong Ye Cundong Ye Kun Jia Kun Jia |
| author_facet | Zihang Ma Zihang Ma Xiaoying Guo Xiaoying Guo Jun Zhang Jun Zhang Jun Zhang Qifeng Jiang Qifeng Jiang Wuying Liang Wuying Liang Wenxin Meng Wenxin Meng Shuaijiang Chen Shuaijiang Chen Yufan Zhu Yufan Zhu Cundong Ye Cundong Ye Cundong Ye Kun Jia Kun Jia |
| author_sort | Zihang Ma |
| collection | DOAJ |
| description | Femoral shaft fracture is a common bone trauma in dogs. The limitation of mesenchymal stem cells in bone defect applications is that the cell suspension cannot be fixed to the bone defect site. In the study, our objective was to substantiate the combined application of canine bone marrow mesenchymal stem cells (cBMSCs) and gelatin-nano-hydroxyapatite (Gel-nHAP) and evaluate its therapeutic effect on bone defect diseases in dogs. Experiments were performed to evaluate the following: (1) the porosity of Gel-nHAP; (2) the adhesion of cBMSCs to Gel-nHAP; and (3) the effect of Gel-nHAP on cBMSC proliferation. The efficacy and safety of the combination of cBMSC and Gel-nHAP in the repair of femoral shaft defects were evaluated in animal experiments. The results showed that Gel-nHAP supported the attachment of cBMSCs and exhibited good biocompatibility. In the animal bone defect repair experiment, significant cortical bone growth was observed in the Gel-nHAP group at week 8 (p < 0.05) and in the cBMSCs-Gel-nHAP group at week 4 (p < 0.01). We demonstrated that Gel-nHAP could promote the repair of bone defects, and the effect of cBMSC-Gel-nHAP on the repair of bone defects was profound. |
| first_indexed | 2024-03-13T04:04:47Z |
| format | Article |
| id | doaj.art-f672e406d66b4bc1acf3dab7b2350aea |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-13T04:04:47Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-f672e406d66b4bc1acf3dab7b2350aea2023-06-21T09:14:10ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-06-011010.3389/fvets.2023.11624071162407Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repairZihang Ma0Zihang Ma1Xiaoying Guo2Xiaoying Guo3Jun Zhang4Jun Zhang5Jun Zhang6Qifeng Jiang7Qifeng Jiang8Wuying Liang9Wuying Liang10Wenxin Meng11Wenxin Meng12Shuaijiang Chen13Shuaijiang Chen14Yufan Zhu15Yufan Zhu16Cundong Ye17Cundong Ye18Cundong Ye19Kun Jia20Kun Jia21College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Technological Engineering Research Center for Pet, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Technological Engineering Research Center for Pet, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Technological Engineering Research Center for Pet, Guangzhou, ChinaGuangdong Polytechnic of Science and Trade, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Technological Engineering Research Center for Pet, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Technological Engineering Research Center for Pet, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Technological Engineering Research Center for Pet, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Technological Engineering Research Center for Pet, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Technological Engineering Research Center for Pet, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Technological Engineering Research Center for Pet, Guangzhou, ChinaCollege of Tropical Agriculture and Forestry, Guangdong Agriculture Industry Business Polytechnic, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Technological Engineering Research Center for Pet, Guangzhou, ChinaFemoral shaft fracture is a common bone trauma in dogs. The limitation of mesenchymal stem cells in bone defect applications is that the cell suspension cannot be fixed to the bone defect site. In the study, our objective was to substantiate the combined application of canine bone marrow mesenchymal stem cells (cBMSCs) and gelatin-nano-hydroxyapatite (Gel-nHAP) and evaluate its therapeutic effect on bone defect diseases in dogs. Experiments were performed to evaluate the following: (1) the porosity of Gel-nHAP; (2) the adhesion of cBMSCs to Gel-nHAP; and (3) the effect of Gel-nHAP on cBMSC proliferation. The efficacy and safety of the combination of cBMSC and Gel-nHAP in the repair of femoral shaft defects were evaluated in animal experiments. The results showed that Gel-nHAP supported the attachment of cBMSCs and exhibited good biocompatibility. In the animal bone defect repair experiment, significant cortical bone growth was observed in the Gel-nHAP group at week 8 (p < 0.05) and in the cBMSCs-Gel-nHAP group at week 4 (p < 0.01). We demonstrated that Gel-nHAP could promote the repair of bone defects, and the effect of cBMSC-Gel-nHAP on the repair of bone defects was profound.https://www.frontiersin.org/articles/10.3389/fvets.2023.1162407/fullcanine bone marrow mesenchymal stem cellsgelatinhydroxyapatitebone defectco-culturebone tissue engineering |
| spellingShingle | Zihang Ma Zihang Ma Xiaoying Guo Xiaoying Guo Jun Zhang Jun Zhang Jun Zhang Qifeng Jiang Qifeng Jiang Wuying Liang Wuying Liang Wenxin Meng Wenxin Meng Shuaijiang Chen Shuaijiang Chen Yufan Zhu Yufan Zhu Cundong Ye Cundong Ye Cundong Ye Kun Jia Kun Jia Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair Frontiers in Veterinary Science canine bone marrow mesenchymal stem cells gelatin hydroxyapatite bone defect co-culture bone tissue engineering |
| title | Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair |
| title_full | Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair |
| title_fullStr | Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair |
| title_full_unstemmed | Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair |
| title_short | Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair |
| title_sort | evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin nano hydroxyapatite combination in canine femoral defect repair |
| topic | canine bone marrow mesenchymal stem cells gelatin hydroxyapatite bone defect co-culture bone tissue engineering |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1162407/full |
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