In Vitro Efficiency of Antimicrobial Peptides against Staphylococcal Pathogens Associated with Canine Pyoderma
The emergence of staphylococcal canine pathogens resistant to multiple antimicrobial agents is a growing and urgent problem in veterinary practice. Antimicrobial peptides (AMPs) seem to be a promising alternative to conventional antibiotics. The aim of this in vitro study was to evaluate the antimic...
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MDPI AG
2020-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/3/470 |
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| author | Małgorzata Jarosiewicz Katarzyna Garbacz Damian Neubauer Wojciech Kamysz |
| author_facet | Małgorzata Jarosiewicz Katarzyna Garbacz Damian Neubauer Wojciech Kamysz |
| author_sort | Małgorzata Jarosiewicz |
| collection | DOAJ |
| description | The emergence of staphylococcal canine pathogens resistant to multiple antimicrobial agents is a growing and urgent problem in veterinary practice. Antimicrobial peptides (AMPs) seem to be a promising alternative to conventional antibiotics. The aim of this in vitro study was to evaluate the antimicrobial activity of selected AMPs against pathogenic staphylococcal strains, including multidrug- and methicillin-resistant strains isolated from canine pyoderma cases. Seven antimicrobial peptides (aurein 1.2, CAMEL, citropin 1.1, protegrin-1, pexiganan, temporin A and uperin 3.6) synthesized by the 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase method were tested. The minimal inhibitory and minimal bactericidal concentrations (MIC and MBC) were determined by the broth microdilution method. The study showed that analyzed AMPs exerted an extensive effect against canine pathogens, with the most active peptide being uperin 3.6. The tested AMPs were equally efficient against both resistant- and susceptible staphylococcal strains and were more efficient against <i>Staphylococcus pseudintermedius</i> than against <i>Staphylococcus aureus</i> strains. Our findings are particularly interesting from a clinical perspective, as they point to AMPs as potential therapeutic topical agents in canine pyoderma cases associated with antimicrobial resistance of staphylococci. |
| first_indexed | 2024-04-12T00:12:09Z |
| format | Article |
| id | doaj.art-f67306e6b1bc4517abded8e9e73e5cc9 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-04-12T00:12:09Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-f67306e6b1bc4517abded8e9e73e5cc92022-12-22T03:55:57ZengMDPI AGAnimals2076-26152020-03-0110347010.3390/ani10030470ani10030470In Vitro Efficiency of Antimicrobial Peptides against Staphylococcal Pathogens Associated with Canine PyodermaMałgorzata Jarosiewicz0Katarzyna Garbacz1Damian Neubauer2Wojciech Kamysz3Department of Oral Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, 25 Dębowa Str., 80-204 Gdansk, PolandDepartment of Oral Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, 25 Dębowa Str., 80-204 Gdansk, PolandDepartment of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdansk, Gdansk, 107 Gen. J. Hallera Str., 80-416 Gdansk, PolandDepartment of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdansk, Gdansk, 107 Gen. J. Hallera Str., 80-416 Gdansk, PolandThe emergence of staphylococcal canine pathogens resistant to multiple antimicrobial agents is a growing and urgent problem in veterinary practice. Antimicrobial peptides (AMPs) seem to be a promising alternative to conventional antibiotics. The aim of this in vitro study was to evaluate the antimicrobial activity of selected AMPs against pathogenic staphylococcal strains, including multidrug- and methicillin-resistant strains isolated from canine pyoderma cases. Seven antimicrobial peptides (aurein 1.2, CAMEL, citropin 1.1, protegrin-1, pexiganan, temporin A and uperin 3.6) synthesized by the 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase method were tested. The minimal inhibitory and minimal bactericidal concentrations (MIC and MBC) were determined by the broth microdilution method. The study showed that analyzed AMPs exerted an extensive effect against canine pathogens, with the most active peptide being uperin 3.6. The tested AMPs were equally efficient against both resistant- and susceptible staphylococcal strains and were more efficient against <i>Staphylococcus pseudintermedius</i> than against <i>Staphylococcus aureus</i> strains. Our findings are particularly interesting from a clinical perspective, as they point to AMPs as potential therapeutic topical agents in canine pyoderma cases associated with antimicrobial resistance of staphylococci.https://www.mdpi.com/2076-2615/10/3/470antimicrobial peptidescanine pyoderma<i>staphylococcus pseudintermedius</i> |
| spellingShingle | Małgorzata Jarosiewicz Katarzyna Garbacz Damian Neubauer Wojciech Kamysz In Vitro Efficiency of Antimicrobial Peptides against Staphylococcal Pathogens Associated with Canine Pyoderma Animals antimicrobial peptides canine pyoderma <i>staphylococcus pseudintermedius</i> |
| title | In Vitro Efficiency of Antimicrobial Peptides against Staphylococcal Pathogens Associated with Canine Pyoderma |
| title_full | In Vitro Efficiency of Antimicrobial Peptides against Staphylococcal Pathogens Associated with Canine Pyoderma |
| title_fullStr | In Vitro Efficiency of Antimicrobial Peptides against Staphylococcal Pathogens Associated with Canine Pyoderma |
| title_full_unstemmed | In Vitro Efficiency of Antimicrobial Peptides against Staphylococcal Pathogens Associated with Canine Pyoderma |
| title_short | In Vitro Efficiency of Antimicrobial Peptides against Staphylococcal Pathogens Associated with Canine Pyoderma |
| title_sort | in vitro efficiency of antimicrobial peptides against staphylococcal pathogens associated with canine pyoderma |
| topic | antimicrobial peptides canine pyoderma <i>staphylococcus pseudintermedius</i> |
| url | https://www.mdpi.com/2076-2615/10/3/470 |
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