Assessment of Mineral Nutrient Efficiency in Genetically Diverse Spinach Accessions by Biochemical and Functional Marker Strategies
Leafy vegetable crops are considered as a natural source of mineral nutrients that could decrease the risk factor of many growth issues in children and adults. Spinach is globally considered as the most desirable leafy crop, due to its taste and nutrient richness along with greater nitrate contents...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-05-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.889604/full |
| _version_ | 1811254337431666688 |
|---|---|
| author | Madiha Rashid Madiha Rashid Madiha Rashid Zubaida Yousaf Ahmad Din Muneeb Munawar Arusa Aftab Nadia Riaz Afifa Younas Ibrahim A. Alaraidh Mohammad K. Okla Hamada AbdElgawad |
| author_facet | Madiha Rashid Madiha Rashid Madiha Rashid Zubaida Yousaf Ahmad Din Muneeb Munawar Arusa Aftab Nadia Riaz Afifa Younas Ibrahim A. Alaraidh Mohammad K. Okla Hamada AbdElgawad |
| author_sort | Madiha Rashid |
| collection | DOAJ |
| description | Leafy vegetable crops are considered as a natural source of mineral nutrients that could decrease the risk factor of many growth issues in children and adults. Spinach is globally considered as the most desirable leafy crop, due to its taste and nutrient richness along with greater nitrate contents and better nitrogen use efficiency. To evaluate the mineral nutrient efficiency of this crop, thirty genetically diverse spinach accessions were analyzed through nutritional and functional marker strategies. The accession 163,310 from Pakistan was found to be rich in minerals (sodium, calcium, potassium, zinc, and manganese) and nitrates. However, the oxalate contents were lesser in the accessions that had greater quantity of nutrients. These represented a negative correlation between mineral availability and oxalate accumulation in the leaves. To study the relationship of oxalates and minerals in the accessions, a functional marker analysis was performed, based on the genes involved in oxalate metabolism and disease resistance in spinach. High level of genetic polymorphism was observed among the accessions represented with 115 polymorphic bands out of 130 bands. Heat map clustering represented the accessions from Asian countries (Pakistan, India, China, and Iran) as the most adaptable accessions to the local environment. The correlation between nutritional and genetic analysis also revealed the nutrient richness of these accessions along with good oxalate metabolism and disease resistance. Hence, these accessions could be considered as useful genotypes in future breeding programs. |
| first_indexed | 2024-04-12T17:05:50Z |
| format | Article |
| id | doaj.art-f6756eca2199416f89f7543a54b6a2b6 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T17:05:50Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f6756eca2199416f89f7543a54b6a2b62022-12-22T03:23:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.889604889604Assessment of Mineral Nutrient Efficiency in Genetically Diverse Spinach Accessions by Biochemical and Functional Marker StrategiesMadiha Rashid0Madiha Rashid1Madiha Rashid2Zubaida Yousaf3Ahmad Din4Muneeb Munawar5Arusa Aftab6Nadia Riaz7Afifa Younas8Ibrahim A. Alaraidh9Mohammad K. Okla10Hamada AbdElgawad11Department of Botany, Lahore College for Women University, Lahore, PakistanDepartment of Botany, Division of Science and Technology, University of Education, Lahore, PakistanInstitute of Biological Sciences, University of Malaya, Kuala Lumpur, MalaysiaDepartment of Botany, Lahore College for Women University, Lahore, PakistanNational Institute of Food Science and Technology, Faculty of Food, Nutrition and Home Sciences, University of Agriculture, Faisalabad, PakistanVegetable Research Institute, Ayub Agricultural Research Institute, Faisalabad, PakistanDepartment of Botany, Lahore College for Women University, Lahore, PakistanDepartment of Botany, Lahore College for Women University, Lahore, PakistanDepartment of Botany, Lahore College for Women University, Lahore, PakistanDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaIntegrated Molecular Plant Physiology Research Group, Department of Biology, University of Antwerp, Antwerp, BelgiumLeafy vegetable crops are considered as a natural source of mineral nutrients that could decrease the risk factor of many growth issues in children and adults. Spinach is globally considered as the most desirable leafy crop, due to its taste and nutrient richness along with greater nitrate contents and better nitrogen use efficiency. To evaluate the mineral nutrient efficiency of this crop, thirty genetically diverse spinach accessions were analyzed through nutritional and functional marker strategies. The accession 163,310 from Pakistan was found to be rich in minerals (sodium, calcium, potassium, zinc, and manganese) and nitrates. However, the oxalate contents were lesser in the accessions that had greater quantity of nutrients. These represented a negative correlation between mineral availability and oxalate accumulation in the leaves. To study the relationship of oxalates and minerals in the accessions, a functional marker analysis was performed, based on the genes involved in oxalate metabolism and disease resistance in spinach. High level of genetic polymorphism was observed among the accessions represented with 115 polymorphic bands out of 130 bands. Heat map clustering represented the accessions from Asian countries (Pakistan, India, China, and Iran) as the most adaptable accessions to the local environment. The correlation between nutritional and genetic analysis also revealed the nutrient richness of these accessions along with good oxalate metabolism and disease resistance. Hence, these accessions could be considered as useful genotypes in future breeding programs.https://www.frontiersin.org/articles/10.3389/fpls.2022.889604/fullaccessionbreedingcrop improvementnitratemineral uptakeoxalate |
| spellingShingle | Madiha Rashid Madiha Rashid Madiha Rashid Zubaida Yousaf Ahmad Din Muneeb Munawar Arusa Aftab Nadia Riaz Afifa Younas Ibrahim A. Alaraidh Mohammad K. Okla Hamada AbdElgawad Assessment of Mineral Nutrient Efficiency in Genetically Diverse Spinach Accessions by Biochemical and Functional Marker Strategies Frontiers in Plant Science accession breeding crop improvement nitrate mineral uptake oxalate |
| title | Assessment of Mineral Nutrient Efficiency in Genetically Diverse Spinach Accessions by Biochemical and Functional Marker Strategies |
| title_full | Assessment of Mineral Nutrient Efficiency in Genetically Diverse Spinach Accessions by Biochemical and Functional Marker Strategies |
| title_fullStr | Assessment of Mineral Nutrient Efficiency in Genetically Diverse Spinach Accessions by Biochemical and Functional Marker Strategies |
| title_full_unstemmed | Assessment of Mineral Nutrient Efficiency in Genetically Diverse Spinach Accessions by Biochemical and Functional Marker Strategies |
| title_short | Assessment of Mineral Nutrient Efficiency in Genetically Diverse Spinach Accessions by Biochemical and Functional Marker Strategies |
| title_sort | assessment of mineral nutrient efficiency in genetically diverse spinach accessions by biochemical and functional marker strategies |
| topic | accession breeding crop improvement nitrate mineral uptake oxalate |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.889604/full |
| work_keys_str_mv | AT madiharashid assessmentofmineralnutrientefficiencyingeneticallydiversespinachaccessionsbybiochemicalandfunctionalmarkerstrategies AT madiharashid assessmentofmineralnutrientefficiencyingeneticallydiversespinachaccessionsbybiochemicalandfunctionalmarkerstrategies AT madiharashid assessmentofmineralnutrientefficiencyingeneticallydiversespinachaccessionsbybiochemicalandfunctionalmarkerstrategies AT zubaidayousaf assessmentofmineralnutrientefficiencyingeneticallydiversespinachaccessionsbybiochemicalandfunctionalmarkerstrategies AT ahmaddin assessmentofmineralnutrientefficiencyingeneticallydiversespinachaccessionsbybiochemicalandfunctionalmarkerstrategies AT muneebmunawar assessmentofmineralnutrientefficiencyingeneticallydiversespinachaccessionsbybiochemicalandfunctionalmarkerstrategies AT arusaaftab assessmentofmineralnutrientefficiencyingeneticallydiversespinachaccessionsbybiochemicalandfunctionalmarkerstrategies AT nadiariaz assessmentofmineralnutrientefficiencyingeneticallydiversespinachaccessionsbybiochemicalandfunctionalmarkerstrategies AT afifayounas assessmentofmineralnutrientefficiencyingeneticallydiversespinachaccessionsbybiochemicalandfunctionalmarkerstrategies AT ibrahimaalaraidh assessmentofmineralnutrientefficiencyingeneticallydiversespinachaccessionsbybiochemicalandfunctionalmarkerstrategies AT mohammadkokla assessmentofmineralnutrientefficiencyingeneticallydiversespinachaccessionsbybiochemicalandfunctionalmarkerstrategies AT hamadaabdelgawad assessmentofmineralnutrientefficiencyingeneticallydiversespinachaccessionsbybiochemicalandfunctionalmarkerstrategies |