Molecular epidemiology of rhinovirus among hospitalised patients, Singapore
Human rhinovirus (HRV) is the most prevalent respiratory etiological agent in the world. Over 100 genotypes have been characterised using molecular genotyping techniques. Here, we characterised the molecular epidemiology of the circulating rhinoviruses among hospitalised patients in Singapore by seq...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2016-12-01
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| Series: | Microbiologia Medica |
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| Online Access: | http://www.pagepressjournals.org/index.php/mm/article/view/6233 |
| _version_ | 1818492880921034752 |
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| author | Chun Kiat Lee Erik Wei Jun Low Christian Benjamin George Highfield Hong Kai Lee Paul Tambyah Anantharajah Tze Ping Loh Evelyn Siew-Chuan Koay |
| author_facet | Chun Kiat Lee Erik Wei Jun Low Christian Benjamin George Highfield Hong Kai Lee Paul Tambyah Anantharajah Tze Ping Loh Evelyn Siew-Chuan Koay |
| author_sort | Chun Kiat Lee |
| collection | DOAJ |
| description | Human rhinovirus (HRV) is the most prevalent respiratory etiological agent in the world. Over 100 genotypes have been characterised using molecular genotyping techniques. Here, we characterised the molecular epidemiology of the circulating rhinoviruses among hospitalised patients in Singapore by sequencing 134 rhinovirus-positive respiratory specimens that were collected in the period between 2013 and 2015. Each sequence was assigned a genogroup and a genotype using the Enterovirus Genotyping Tool Version 0.1 and phylogenetic reconstruction, respectively. In this study, HRV-A (n=88) and HRV-C (n=38) were identified as the dominant genogroups in Singapore. HRV-A28 (n=7) was the dominant genotype in HRV-A while both HRVC2 (n=8) and HRV-C11 (n=8) were the dominant genotypes in HRV-C. HRV-B was observed to have the lowest number of positive detections in our study population (n=8). The result is interesting as another group had previously found HRV-B to be the second most common genogroup in Singapore after HRV-A. |
| first_indexed | 2024-12-10T17:48:06Z |
| format | Article |
| id | doaj.art-f675d46b870f4cb0831101fb36d9ca91 |
| institution | Directory Open Access Journal |
| issn | 2280-6423 |
| language | English |
| last_indexed | 2024-12-10T17:48:06Z |
| publishDate | 2016-12-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Microbiologia Medica |
| spelling | doaj.art-f675d46b870f4cb0831101fb36d9ca912022-12-22T01:39:10ZengPAGEPress PublicationsMicrobiologia Medica2280-64232016-12-0131410.4081/mm.2016.62335068Molecular epidemiology of rhinovirus among hospitalised patients, SingaporeChun Kiat Lee0Erik Wei Jun Low1Christian Benjamin George Highfield2Hong Kai Lee3Paul Tambyah Anantharajah4Tze Ping Loh5Evelyn Siew-Chuan Koay6Department of Laboratory Medicine, National University Hospital, SingaporeNgee Ann Polytechnic, SingaporeNgee Ann Polytechnic, SingaporeDepartment of Laboratory Medicine, National University Hospital, SingaporeDepartment of Medicine, Yong Loo Lin School of Medicine, National University of SingaporeDepartment of Laboratory Medicine, National University Hospital, SingaporeDepartment of Laboratory Medicine, and Department of Pathology, Yong Loo Lin School of Medicine, National University of SingaporeHuman rhinovirus (HRV) is the most prevalent respiratory etiological agent in the world. Over 100 genotypes have been characterised using molecular genotyping techniques. Here, we characterised the molecular epidemiology of the circulating rhinoviruses among hospitalised patients in Singapore by sequencing 134 rhinovirus-positive respiratory specimens that were collected in the period between 2013 and 2015. Each sequence was assigned a genogroup and a genotype using the Enterovirus Genotyping Tool Version 0.1 and phylogenetic reconstruction, respectively. In this study, HRV-A (n=88) and HRV-C (n=38) were identified as the dominant genogroups in Singapore. HRV-A28 (n=7) was the dominant genotype in HRV-A while both HRVC2 (n=8) and HRV-C11 (n=8) were the dominant genotypes in HRV-C. HRV-B was observed to have the lowest number of positive detections in our study population (n=8). The result is interesting as another group had previously found HRV-B to be the second most common genogroup in Singapore after HRV-A.http://www.pagepressjournals.org/index.php/mm/article/view/6233Respiratory tract infectionsrhinovirusgenotypingRT-PCRepidemiologymolecular diagnostics |
| spellingShingle | Chun Kiat Lee Erik Wei Jun Low Christian Benjamin George Highfield Hong Kai Lee Paul Tambyah Anantharajah Tze Ping Loh Evelyn Siew-Chuan Koay Molecular epidemiology of rhinovirus among hospitalised patients, Singapore Microbiologia Medica Respiratory tract infections rhinovirus genotyping RT-PCR epidemiology molecular diagnostics |
| title | Molecular epidemiology of rhinovirus among hospitalised patients, Singapore |
| title_full | Molecular epidemiology of rhinovirus among hospitalised patients, Singapore |
| title_fullStr | Molecular epidemiology of rhinovirus among hospitalised patients, Singapore |
| title_full_unstemmed | Molecular epidemiology of rhinovirus among hospitalised patients, Singapore |
| title_short | Molecular epidemiology of rhinovirus among hospitalised patients, Singapore |
| title_sort | molecular epidemiology of rhinovirus among hospitalised patients singapore |
| topic | Respiratory tract infections rhinovirus genotyping RT-PCR epidemiology molecular diagnostics |
| url | http://www.pagepressjournals.org/index.php/mm/article/view/6233 |
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