Dynamic changes in genomic 5-hydroxymethyluracil and N6-methyladenine levels in the Drosophila melanogaster life cycle and in response to different temperature conditions
Abstract In this study, the level of DNA modifications was investigated in three developmental stages of Drosophila melanogaster (larvae, pupae, imago) and in an in vitro model (Schneider 2 cells). Analysis was carried out using two-dimensional ultra-performance liquid chromatography with tandem mas...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-10-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-22490-9 |
| _version_ | 1811249899389321216 |
|---|---|
| author | Marta Starczak Maciej Gawronski Aleksandra Wasilow Pawel Mijewski Ryszard Olinski Daniel Gackowski |
| author_facet | Marta Starczak Maciej Gawronski Aleksandra Wasilow Pawel Mijewski Ryszard Olinski Daniel Gackowski |
| author_sort | Marta Starczak |
| collection | DOAJ |
| description | Abstract In this study, the level of DNA modifications was investigated in three developmental stages of Drosophila melanogaster (larvae, pupae, imago) and in an in vitro model (Schneider 2 cells). Analysis was carried out using two-dimensional ultra-performance liquid chromatography with tandem mass spectrometry. Our method made it possible, for the first time, to analyze a broad spectrum of DNA modifications in the three stages of Drosophila. Each stage was characterized by a specific modification pattern, and the levels of these compounds fluctuated throughout the D. melanogaster life cycle. The level of DNA modification was also compared between insects bred at 25 °C (optimal temperature) and at 18 °C, and the groups differed significantly. The profound changes in N6-methyladenine and 5-hydroxymethyluracil levels during the Drosophila life cycle and as a result of breeding temperature changes indicate that these DNA modifications can play important regulatory roles in response to environmental changes and/or biological conditions. Moreover, the supplementation of Schneider 2 cells with 1 mM L-ascorbic acid caused a time-dependent increase in the level of 5-(hydroxymethyl)-2′-deoxyuridine. These data suggest that a certain pool of this compound may arise from the enzymatic activity of the dTET protein. |
| first_indexed | 2024-04-12T15:55:36Z |
| format | Article |
| id | doaj.art-f67905b556f74ee79dc7f8c15dd10601 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T15:55:36Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f67905b556f74ee79dc7f8c15dd106012022-12-22T03:26:23ZengNature PortfolioScientific Reports2045-23222022-10-0112111310.1038/s41598-022-22490-9Dynamic changes in genomic 5-hydroxymethyluracil and N6-methyladenine levels in the Drosophila melanogaster life cycle and in response to different temperature conditionsMarta Starczak0Maciej Gawronski1Aleksandra Wasilow2Pawel Mijewski3Ryszard Olinski4Daniel Gackowski5Department of Clinical Biochemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in ToruńDepartment of Clinical Biochemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in ToruńDepartment of Clinical Biochemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in ToruńDepartment of Clinical Biochemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in ToruńDepartment of Clinical Biochemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in ToruńDepartment of Clinical Biochemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in ToruńAbstract In this study, the level of DNA modifications was investigated in three developmental stages of Drosophila melanogaster (larvae, pupae, imago) and in an in vitro model (Schneider 2 cells). Analysis was carried out using two-dimensional ultra-performance liquid chromatography with tandem mass spectrometry. Our method made it possible, for the first time, to analyze a broad spectrum of DNA modifications in the three stages of Drosophila. Each stage was characterized by a specific modification pattern, and the levels of these compounds fluctuated throughout the D. melanogaster life cycle. The level of DNA modification was also compared between insects bred at 25 °C (optimal temperature) and at 18 °C, and the groups differed significantly. The profound changes in N6-methyladenine and 5-hydroxymethyluracil levels during the Drosophila life cycle and as a result of breeding temperature changes indicate that these DNA modifications can play important regulatory roles in response to environmental changes and/or biological conditions. Moreover, the supplementation of Schneider 2 cells with 1 mM L-ascorbic acid caused a time-dependent increase in the level of 5-(hydroxymethyl)-2′-deoxyuridine. These data suggest that a certain pool of this compound may arise from the enzymatic activity of the dTET protein.https://doi.org/10.1038/s41598-022-22490-9 |
| spellingShingle | Marta Starczak Maciej Gawronski Aleksandra Wasilow Pawel Mijewski Ryszard Olinski Daniel Gackowski Dynamic changes in genomic 5-hydroxymethyluracil and N6-methyladenine levels in the Drosophila melanogaster life cycle and in response to different temperature conditions Scientific Reports |
| title | Dynamic changes in genomic 5-hydroxymethyluracil and N6-methyladenine levels in the Drosophila melanogaster life cycle and in response to different temperature conditions |
| title_full | Dynamic changes in genomic 5-hydroxymethyluracil and N6-methyladenine levels in the Drosophila melanogaster life cycle and in response to different temperature conditions |
| title_fullStr | Dynamic changes in genomic 5-hydroxymethyluracil and N6-methyladenine levels in the Drosophila melanogaster life cycle and in response to different temperature conditions |
| title_full_unstemmed | Dynamic changes in genomic 5-hydroxymethyluracil and N6-methyladenine levels in the Drosophila melanogaster life cycle and in response to different temperature conditions |
| title_short | Dynamic changes in genomic 5-hydroxymethyluracil and N6-methyladenine levels in the Drosophila melanogaster life cycle and in response to different temperature conditions |
| title_sort | dynamic changes in genomic 5 hydroxymethyluracil and n6 methyladenine levels in the drosophila melanogaster life cycle and in response to different temperature conditions |
| url | https://doi.org/10.1038/s41598-022-22490-9 |
| work_keys_str_mv | AT martastarczak dynamicchangesingenomic5hydroxymethyluracilandn6methyladeninelevelsinthedrosophilamelanogasterlifecycleandinresponsetodifferenttemperatureconditions AT maciejgawronski dynamicchangesingenomic5hydroxymethyluracilandn6methyladeninelevelsinthedrosophilamelanogasterlifecycleandinresponsetodifferenttemperatureconditions AT aleksandrawasilow dynamicchangesingenomic5hydroxymethyluracilandn6methyladeninelevelsinthedrosophilamelanogasterlifecycleandinresponsetodifferenttemperatureconditions AT pawelmijewski dynamicchangesingenomic5hydroxymethyluracilandn6methyladeninelevelsinthedrosophilamelanogasterlifecycleandinresponsetodifferenttemperatureconditions AT ryszardolinski dynamicchangesingenomic5hydroxymethyluracilandn6methyladeninelevelsinthedrosophilamelanogasterlifecycleandinresponsetodifferenttemperatureconditions AT danielgackowski dynamicchangesingenomic5hydroxymethyluracilandn6methyladeninelevelsinthedrosophilamelanogasterlifecycleandinresponsetodifferenttemperatureconditions |