NASCarD (Nanopore Adaptive Sampling with Carrier DNA): A Rapid, PCR-Free Method for SARS-CoV-2 Whole-Genome Sequencing in Clinical Samples

NASCarD (Nanopore Adaptive Sampling with Carrier DNA): A Rapid, PCR-Free Method for SARS-CoV-2 Whole-Genome Sequencing in Clinical Samples

Whole-genome sequencing (WGS) represents the main technology for SARS-CoV-2 lineage characterization in diagnostic laboratories worldwide. The rapid, near-full-length sequencing of the viral genome is commonly enabled by high-throughput sequencing of PCR amplicons derived from cDNA molecules. Here,...

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Bibliographic Details
Main Authors: Miguel A. Terrazos Miani, Loïc Borcard, Sonja Gempeler, Christian Baumann, Pascal Bittel, Stephen L. Leib, Stefan Neuenschwander, Alban Ramette
Format: Article
Language:English
Published: MDPI AG 2024-01-01
Series:Pathogens
Subjects:
beta coronaviruses
SARS-CoV-2
whole-genome sequencing
next-generation sequencing
genomics
nanopore sequencing
Online Access:https://www.mdpi.com/2076-0817/13/1/61

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