A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption
We have investigated urine samples after coffee consumption using targeted and untargeted approaches to identify furan and 2-methylfuran metabolites in urine samples by UPLC-qToF. The aim was to establish a fast, robust, and time-saving method involving ultra-performance liquid chromatography-quanti...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/21/5104 |
| _version_ | 1797548915763970048 |
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| author | Simone Stegmüller Nadine Beißmann Jonathan Isaak Kremer Denise Mehl Christian Baumann Elke Richling |
| author_facet | Simone Stegmüller Nadine Beißmann Jonathan Isaak Kremer Denise Mehl Christian Baumann Elke Richling |
| author_sort | Simone Stegmüller |
| collection | DOAJ |
| description | We have investigated urine samples after coffee consumption using targeted and untargeted approaches to identify furan and 2-methylfuran metabolites in urine samples by UPLC-qToF. The aim was to establish a fast, robust, and time-saving method involving ultra-performance liquid chromatography-quantitative time-of-flight tandem mass spectrometry (UPLC-qToF-MS/MS). The developed method detected previously reported metabolites, such as Lys-BDA, and others that had not been previously identified, or only detected in animal or in vitro studies. The developed UPLC-qToF method detected previously reported metabolites, such as lysine-<i>cis</i>-2-butene-1,4-dial (Lys-BDA) adducts, and others that had not been previously identified, or only detected in animal and in vitro studies. In sum, the UPLC-qToF approach provides additional information that may be valuable in future human or animal intervention studies. |
| first_indexed | 2024-03-10T15:07:35Z |
| format | Article |
| id | doaj.art-f67dd064137d443ca99551232f3db149 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T15:07:35Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f67dd064137d443ca99551232f3db1492023-11-20T19:40:23ZengMDPI AGMolecules1420-30492020-11-012521510410.3390/molecules25215104A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee ConsumptionSimone Stegmüller0Nadine Beißmann1Jonathan Isaak Kremer2Denise Mehl3Christian Baumann4Elke Richling5Technische Universität Kaiserslautern, Department of Chemistry, Division of Food Chemistry and Toxicology, Erwin-Schrödinger-Str. 52, 67663 Kaiserslautern, GermanyTechnische Universität Kaiserslautern, Department of Chemistry, Division of Food Chemistry and Toxicology, Erwin-Schrödinger-Str. 52, 67663 Kaiserslautern, GermanyTechnische Universität Kaiserslautern, Department of Chemistry, Division of Food Chemistry and Toxicology, Erwin-Schrödinger-Str. 52, 67663 Kaiserslautern, GermanyAB SCIEX Germany GmbH, 64293 Darmstadt, GermanyAB SCIEX Germany GmbH, 64293 Darmstadt, GermanyTechnische Universität Kaiserslautern, Department of Chemistry, Division of Food Chemistry and Toxicology, Erwin-Schrödinger-Str. 52, 67663 Kaiserslautern, GermanyWe have investigated urine samples after coffee consumption using targeted and untargeted approaches to identify furan and 2-methylfuran metabolites in urine samples by UPLC-qToF. The aim was to establish a fast, robust, and time-saving method involving ultra-performance liquid chromatography-quantitative time-of-flight tandem mass spectrometry (UPLC-qToF-MS/MS). The developed method detected previously reported metabolites, such as Lys-BDA, and others that had not been previously identified, or only detected in animal or in vitro studies. The developed UPLC-qToF method detected previously reported metabolites, such as lysine-<i>cis</i>-2-butene-1,4-dial (Lys-BDA) adducts, and others that had not been previously identified, or only detected in animal and in vitro studies. In sum, the UPLC-qToF approach provides additional information that may be valuable in future human or animal intervention studies.https://www.mdpi.com/1420-3049/25/21/5104furan2-methylfuranUPLC-qToFuntargeted analysisurinary metabolites |
| spellingShingle | Simone Stegmüller Nadine Beißmann Jonathan Isaak Kremer Denise Mehl Christian Baumann Elke Richling A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption Molecules furan 2-methylfuran UPLC-qToF untargeted analysis urinary metabolites |
| title | A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption |
| title_full | A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption |
| title_fullStr | A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption |
| title_full_unstemmed | A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption |
| title_short | A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption |
| title_sort | new uplc qtof approach for elucidating furan and 2 methylfuran metabolites in human urine samples after coffee consumption |
| topic | furan 2-methylfuran UPLC-qToF untargeted analysis urinary metabolites |
| url | https://www.mdpi.com/1420-3049/25/21/5104 |
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