Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya)
The aim of this work was extract, characterize and evaluate the antibacterial activity of the essential oil of Senecio graveolens Wedd (Wiskataya) against Escherichia coli and Staphylococcus aureus ATCC 29923. The leaves and stems of S. graveolens were collected in the district of Puquio 3800 m.s.n....
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| Format: | Article |
| Language: | English |
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Universidad Nacional de Trujillo
2012-12-01
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| Series: | Scientia Agropecuaria |
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| Online Access: | http://www.revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/90 |
| _version_ | 1818488270264205312 |
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| author | Kiev Ochoa Pumaylle Luis Ricardo Paredes Quiroz Dagnith Liz Bejarano Luján Reynaldo Justino Silva Paz |
| author_facet | Kiev Ochoa Pumaylle Luis Ricardo Paredes Quiroz Dagnith Liz Bejarano Luján Reynaldo Justino Silva Paz |
| author_sort | Kiev Ochoa Pumaylle |
| collection | DOAJ |
| description | The aim of this work was extract, characterize and evaluate the antibacterial activity of the essential oil of Senecio graveolens Wedd (Wiskataya) against Escherichia coli and Staphylococcus aureus ATCC 29923. The leaves and stems of S. graveolens were collected in the district of Puquio 3800 m.s.n.m., Lucanas province, and department of Ayacucho. The essential oil was obtained by steam water destilation dried leaves and stems with yield 1.26% (w/w) to which physical testing were performed. The chemical composition was evaluated by gas chromatography with mass detector (GC-MS). Antibacterial activity of S. graveolens oil was tested by agar diffusion method in wells against Gram positive strains such as S. aureus ATCC 29923 and Gram negative as E. coli. The density a 20 ºC was 0.8755 g/ml; the index refraction was 1.4726 and the rate rotation was 102° 85' and the solubility miscible in ethanol. The GC-MS showed the main components sabinene (52.39 %), (+)-4-carene (8.20 %), τ-terpinen (7.11 %), β-myrcene (6.74 %), 4-terpinenol (3.78 %) and pulegone (3.67 %). The results showing activity strong antibacterial activity and moderate, respectively, for the strains tested, observing formation of inhibition halos for essential oil concentrations at 80, 90 and 100 % in both strains. The essential oil of S. graveolens presented with promising antibacterial activity. |
| first_indexed | 2024-12-10T16:48:47Z |
| format | Article |
| id | doaj.art-f67e652c45a843488ab12e9d688ff5ec |
| institution | Directory Open Access Journal |
| issn | 2077-9917 2306-6741 |
| language | English |
| last_indexed | 2024-12-10T16:48:47Z |
| publishDate | 2012-12-01 |
| publisher | Universidad Nacional de Trujillo |
| record_format | Article |
| series | Scientia Agropecuaria |
| spelling | doaj.art-f67e652c45a843488ab12e9d688ff5ec2022-12-22T01:40:59ZengUniversidad Nacional de TrujilloScientia Agropecuaria2077-99172306-67412012-12-013429130210.17268/sci.agropecu.2012.04.03Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya)Kiev Ochoa PumaylleLuis Ricardo Paredes QuirozDagnith Liz Bejarano LujánReynaldo Justino Silva PazThe aim of this work was extract, characterize and evaluate the antibacterial activity of the essential oil of Senecio graveolens Wedd (Wiskataya) against Escherichia coli and Staphylococcus aureus ATCC 29923. The leaves and stems of S. graveolens were collected in the district of Puquio 3800 m.s.n.m., Lucanas province, and department of Ayacucho. The essential oil was obtained by steam water destilation dried leaves and stems with yield 1.26% (w/w) to which physical testing were performed. The chemical composition was evaluated by gas chromatography with mass detector (GC-MS). Antibacterial activity of S. graveolens oil was tested by agar diffusion method in wells against Gram positive strains such as S. aureus ATCC 29923 and Gram negative as E. coli. The density a 20 ºC was 0.8755 g/ml; the index refraction was 1.4726 and the rate rotation was 102° 85' and the solubility miscible in ethanol. The GC-MS showed the main components sabinene (52.39 %), (+)-4-carene (8.20 %), τ-terpinen (7.11 %), β-myrcene (6.74 %), 4-terpinenol (3.78 %) and pulegone (3.67 %). The results showing activity strong antibacterial activity and moderate, respectively, for the strains tested, observing formation of inhibition halos for essential oil concentrations at 80, 90 and 100 % in both strains. The essential oil of S. graveolens presented with promising antibacterial activity.http://www.revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/90WiskatayaSenecio graveolens Weddessential oilantibacterial activity. |
| spellingShingle | Kiev Ochoa Pumaylle Luis Ricardo Paredes Quiroz Dagnith Liz Bejarano Luján Reynaldo Justino Silva Paz Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya) Scientia Agropecuaria Wiskataya Senecio graveolens Wedd essential oil antibacterial activity. |
| title | Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya) |
| title_full | Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya) |
| title_fullStr | Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya) |
| title_full_unstemmed | Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya) |
| title_short | Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya) |
| title_sort | extraction characterization and evaluation of antibacterial activity of essential oil of senecio graveolens wedd wiskataya |
| topic | Wiskataya Senecio graveolens Wedd essential oil antibacterial activity. |
| url | http://www.revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/90 |
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