A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish
Palythoa caribaeorum (class Anthozoa) is a zoantharian which, together with other cnidarians, like jellyfishes, hydra, and sea anemones, possesses specialized structures in its tissues, the cnidocytes, which deliver an array of toxins in order to capture prey and deter predators. The whole transcrip...
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| Format: | Article |
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MDPI AG
2018-06-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/10/6/238 |
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| author | Qiwen Liao Guiyi Gong Shirley Weng In Siu Clarence Tsun Ting Wong Huidong Yu Yu Chung Tse Gandhi Rádis-Baptista Simon Ming-Yuen Lee |
| author_facet | Qiwen Liao Guiyi Gong Shirley Weng In Siu Clarence Tsun Ting Wong Huidong Yu Yu Chung Tse Gandhi Rádis-Baptista Simon Ming-Yuen Lee |
| author_sort | Qiwen Liao |
| collection | DOAJ |
| description | Palythoa caribaeorum (class Anthozoa) is a zoantharian which, together with other cnidarians, like jellyfishes, hydra, and sea anemones, possesses specialized structures in its tissues, the cnidocytes, which deliver an array of toxins in order to capture prey and deter predators. The whole transcriptome of P. caribaeroum was deep sequenced, and a diversity of toxin-related peptide sequences were identified, and some retrieved for functional analysis. In this work, a peptide precursor containing a ShK domain, named PcShK3, was analyzed by means of computational processing, comprising structural phylogenetic analysis, model prediction, and dynamics simulation of peptide-receptor interaction. The combined data indicated that PcShK3 is a distinct peptide which is homologous to a cluster of peptides belonging to the ShK toxin family. In vivo, PcShK3 distributed across the vitelline membrane and accumulated in the yolk sac stripe of zebrafish larvae. Notably, it displayed a significant cardio-protective effect in zebrafish in concentrations inferior to the IC50 (<43.53 ± 6.45 µM), while in high concentrations (>IC50), it accumulated in the blood and caused pericardial edema, being cardiotoxic to zebrafish larvae. Remarkably, PcShK3 suppressed the 6-OHDA-induced neurotoxicity on the locomotive behavior of zebrafish. The present results indicated that PcShK3 is a novel member of ShK toxin family, and has the intrinsic ability to induce neuro- and cardio-protective effects or cause cardiac toxicity, according to its effective concentration. |
| first_indexed | 2024-04-14T01:39:59Z |
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| id | doaj.art-f67f7e0bb6b34b1ebb3818ecbf5c9e8d |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T01:39:59Z |
| publishDate | 2018-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-f67f7e0bb6b34b1ebb3818ecbf5c9e8d2022-12-22T02:19:49ZengMDPI AGToxins2072-66512018-06-0110623810.3390/toxins10060238toxins10060238A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in ZebrafishQiwen Liao0Guiyi Gong1Shirley Weng In Siu2Clarence Tsun Ting Wong3Huidong Yu4Yu Chung Tse5Gandhi Rádis-Baptista6Simon Ming-Yuen Lee7State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau, ChinaState Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau, ChinaDepartment of Computer and Information Science, Faculty of Science and Technology, University of Macau, Macau, ChinaDepartment of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, ChinaShenzhen Rongxin Biotechnology Co., Ltd., Shenzhen 518054, ChinaDepartment of Biology, South University of Science and Technology of China, Shenzhen 518055, ChinaLaboratory of Biochemistry and Biotechnology, Institute for Marine Sciences, Federal University of Ceará, Fortaleza 60165-081, BrazilState Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau, ChinaPalythoa caribaeorum (class Anthozoa) is a zoantharian which, together with other cnidarians, like jellyfishes, hydra, and sea anemones, possesses specialized structures in its tissues, the cnidocytes, which deliver an array of toxins in order to capture prey and deter predators. The whole transcriptome of P. caribaeroum was deep sequenced, and a diversity of toxin-related peptide sequences were identified, and some retrieved for functional analysis. In this work, a peptide precursor containing a ShK domain, named PcShK3, was analyzed by means of computational processing, comprising structural phylogenetic analysis, model prediction, and dynamics simulation of peptide-receptor interaction. The combined data indicated that PcShK3 is a distinct peptide which is homologous to a cluster of peptides belonging to the ShK toxin family. In vivo, PcShK3 distributed across the vitelline membrane and accumulated in the yolk sac stripe of zebrafish larvae. Notably, it displayed a significant cardio-protective effect in zebrafish in concentrations inferior to the IC50 (<43.53 ± 6.45 µM), while in high concentrations (>IC50), it accumulated in the blood and caused pericardial edema, being cardiotoxic to zebrafish larvae. Remarkably, PcShK3 suppressed the 6-OHDA-induced neurotoxicity on the locomotive behavior of zebrafish. The present results indicated that PcShK3 is a novel member of ShK toxin family, and has the intrinsic ability to induce neuro- and cardio-protective effects or cause cardiac toxicity, according to its effective concentration.http://www.mdpi.com/2072-6651/10/6/238zoantharianShK-like peptidedynamics simulationvoltage-gated K+ ion channelcalcium-activated K+ ion channelanimal toxins |
| spellingShingle | Qiwen Liao Guiyi Gong Shirley Weng In Siu Clarence Tsun Ting Wong Huidong Yu Yu Chung Tse Gandhi Rádis-Baptista Simon Ming-Yuen Lee A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish Toxins zoantharian ShK-like peptide dynamics simulation voltage-gated K+ ion channel calcium-activated K+ ion channel animal toxins |
| title | A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish |
| title_full | A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish |
| title_fullStr | A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish |
| title_full_unstemmed | A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish |
| title_short | A Novel ShK-Like Toxic Peptide from the Transcriptome of the Cnidarian Palythoa caribaeorum Displays Neuroprotection and Cardioprotection in Zebrafish |
| title_sort | novel shk like toxic peptide from the transcriptome of the cnidarian palythoa caribaeorum displays neuroprotection and cardioprotection in zebrafish |
| topic | zoantharian ShK-like peptide dynamics simulation voltage-gated K+ ion channel calcium-activated K+ ion channel animal toxins |
| url | http://www.mdpi.com/2072-6651/10/6/238 |
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