Biochemical and tissue physiopathological evaluation of the preclinical efficacy of Solanum torvum Swartz leaves for treating oxidative impairment in rats administered a β-cell-toxicant (STZ)
The current study evaluated the protective role of Solanum torvum Swartz against diabetes-induced oxidative stress and tissue impairment in streptozotocin (STZ)-intoxicated rats. Rats with STZ (40 mg/kg intraperitoneally (i.p.))-induced diabetes were divided into five groups (n = 5) and treated with...
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Elsevier
2022-10-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332222009945 |
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| author | Saidu Sani Bashir Lawal Jerius N. Ejeje Tawakalitu B. Aliu Amos S. Onikanni Onwe O. Uchewa Joy C. Ovoh Faith U. Ekpa Chikezie D. Ozoagu Tochukwu S. Akuma Success C. Onyeji Amara Obialor Saqer S. Alotaibi Sarah M. Albogami Michel De Waard Gaber El-Saber Batiha Tse Hung Huang Alexander T.H. Wu |
| author_facet | Saidu Sani Bashir Lawal Jerius N. Ejeje Tawakalitu B. Aliu Amos S. Onikanni Onwe O. Uchewa Joy C. Ovoh Faith U. Ekpa Chikezie D. Ozoagu Tochukwu S. Akuma Success C. Onyeji Amara Obialor Saqer S. Alotaibi Sarah M. Albogami Michel De Waard Gaber El-Saber Batiha Tse Hung Huang Alexander T.H. Wu |
| author_sort | Saidu Sani |
| collection | DOAJ |
| description | The current study evaluated the protective role of Solanum torvum Swartz against diabetes-induced oxidative stress and tissue impairment in streptozotocin (STZ)-intoxicated rats. Rats with STZ (40 mg/kg intraperitoneally (i.p.))-induced diabetes were divided into five groups (n = 5) and treated with (i) normal saline, (ii) 150 mg/kg body weight (BW) of the ethanol extract of S. torvum leaf (EESTL), (ii) 300 mg/kg BW EESTL, (iv) 100 mg/kg BW metformin, and (v) 50 m/kg BW metformin + 100 mg/kg BW EESTL orally for 21 days. Our results revealed that the EESTL displayed dose-dependent ferric-reducing antioxidant power (FRAP) activity, scavenged DPPH radicals (IC50) = 13.52 ± 0.45 µg/mL), and inhibited lipid peroxidation in an in vitro models. In addition, the EESTL demonstrated dose-dependent inhibitory activity against α-amylase (IC50 =138.46 ± 3.97 µg/mL) and promoted glucose uptake across plasma membranes of yeast cells in a manner comparable to that of metformin. Interestingly, the extract demonstrated in vivo blood glucose normalization effects with concomitant increased activities of antioxidant parameters (superoxide dismutase (SOD), catalase, and reduced glutathione (GSH)) while decreasing malondialdehyde (MDA) levels when compared to untreated rats. Similarly, serum biochemical alterations, and tissues (liver, kidney, and pancreases) histopathological aberrations in untreated rats with STZ-induced diabetes were attenuated by treatment with the EESTL. Biometabolite characterization of the extract identified gallic acid (45.81 ppm), catechin (1.18 ppm), p-coumaric acid (1.43e−1 ppm), DL-proline 5-oxo-methyl ester (9.16 %, retention time (RT): 8.57 min), salicylic acid (3.26% and 7.61 min), and butylated hydroxytoluene (4.75%, RT: 10.18 min) as the major polyphenolic compounds in the plant extract. In conclusion, our study provides preclinical evidence of the antioxidant properties and oxidative stress-preventing role of S. torvum in STZ-dosed diabetic rats. Taken together, the EESTL represents a reserve of bioactive metabolites for managing diabetes and associated complications. |
| first_indexed | 2024-04-12T04:54:10Z |
| format | Article |
| id | doaj.art-f682671690c3412ab1b07786a3278487 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-12T04:54:10Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-f682671690c3412ab1b07786a32784872022-12-22T03:47:11ZengElsevierBiomedicine & Pharmacotherapy0753-33222022-10-01154113605Biochemical and tissue physiopathological evaluation of the preclinical efficacy of Solanum torvum Swartz leaves for treating oxidative impairment in rats administered a β-cell-toxicant (STZ)Saidu Sani0Bashir Lawal1Jerius N. Ejeje2Tawakalitu B. Aliu3Amos S. Onikanni4Onwe O. Uchewa5Joy C. Ovoh6Faith U. Ekpa7Chikezie D. Ozoagu8Tochukwu S. Akuma9Success C. Onyeji10Amara Obialor11Saqer S. Alotaibi12Sarah M. Albogami13Michel De Waard14Gaber El-Saber Batiha15Tse Hung Huang16Alexander T.H. Wu17Department of Biochemistry, Faculty of Biological Science, Alex Ekwueme Federal University Ndufu Alike Ikwo, PMB 1010 Abakalike, Ebonyi State, NigeriaPhD Program for Cancer Molecular Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University and Academia Sinica, Taipei 11031, Taiwan; Graduate Institute of Cancer Biology & Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, TaiwanDepartment of Biochemistry, Faculty of Biological Science, Alex Ekwueme Federal University Ndufu Alike Ikwo, PMB 1010 Abakalike, Ebonyi State, Nigeria; Biochemistry Unit, Department of Chemical Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, NigeriaDepartment of Medical Biochemistry, Faculty of Basic Medical Sciences, Alex-Ekwueme Federal University Ndufu Alike Ikwo, Ebonyi State, NigeriaBiochemistry Unit, Department of Chemical Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria; Graduate Institute of Biomedical Science, College of Medicine, China Medical University, Taipei, TaiwanDepartment of Human Anatomy, College of Basic Medical Science, Alex Ekwueme Federal University Ndufu Alike Ikwo, PMB 1010 Abakalike, Ebonyi State, NigeriaDepartment of Biochemistry, Faculty of Biological Science, Alex Ekwueme Federal University Ndufu Alike Ikwo, PMB 1010 Abakalike, Ebonyi State, NigeriaDepartment of Biochemistry, Faculty of Biological Science, Alex Ekwueme Federal University Ndufu Alike Ikwo, PMB 1010 Abakalike, Ebonyi State, NigeriaDepartment of Biochemistry, Faculty of Biological Science, Alex Ekwueme Federal University Ndufu Alike Ikwo, PMB 1010 Abakalike, Ebonyi State, NigeriaDepartment of Biochemistry, Faculty of Biological Science, Alex Ekwueme Federal University Ndufu Alike Ikwo, PMB 1010 Abakalike, Ebonyi State, NigeriaDepartment of Biochemistry, Faculty of Biological Science, Alex Ekwueme Federal University Ndufu Alike Ikwo, PMB 1010 Abakalike, Ebonyi State, NigeriaDepartment of Biochemistry, Faculty of Biological Science, Alex Ekwueme Federal University Ndufu Alike Ikwo, PMB 1010 Abakalike, Ebonyi State, NigeriaDepartment of Biotechnology, College of Science, Taif University, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, Taif 21944, Saudi ArabiaSmartox Biotechnology, 6 rue des Platanes, 38120 Saint-Egrève, France; L’institut du Thorax, INSERM, CNRS, UNIV NANTES, F-44007 Nantes, France; LabEx Ion Channels, Science & Therapeutics, Université de Nice Sophia-Antipolis, F-06560 Valbonne, FranceDepartment of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, EgyptDepartment of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Keelung 204, Taiwan; School of Traditional Chinese Medicine, Chang Gung University, Kweishan, Taoyuan 333, Taiwan; School of Nursing, National Taipei University of Nursing and Health Sciences, Taipei 112, Taiwan; Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan; Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan; Department & Graduate Institute of Chemical Engineering & Graduate Institute of Biochemical Engineering, Ming Chi University of Technology, New Taipei City 243, TaiwanThe PhD Program of Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan; TMU Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 11031, Taiwan; Clinical Research Center, Taipei Medical University Hospital, Taipei Medical University, Taipei 11031, Taiwan; Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 114, Taiwan; Corresponding author at: The PhD Program of Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan.The current study evaluated the protective role of Solanum torvum Swartz against diabetes-induced oxidative stress and tissue impairment in streptozotocin (STZ)-intoxicated rats. Rats with STZ (40 mg/kg intraperitoneally (i.p.))-induced diabetes were divided into five groups (n = 5) and treated with (i) normal saline, (ii) 150 mg/kg body weight (BW) of the ethanol extract of S. torvum leaf (EESTL), (ii) 300 mg/kg BW EESTL, (iv) 100 mg/kg BW metformin, and (v) 50 m/kg BW metformin + 100 mg/kg BW EESTL orally for 21 days. Our results revealed that the EESTL displayed dose-dependent ferric-reducing antioxidant power (FRAP) activity, scavenged DPPH radicals (IC50) = 13.52 ± 0.45 µg/mL), and inhibited lipid peroxidation in an in vitro models. In addition, the EESTL demonstrated dose-dependent inhibitory activity against α-amylase (IC50 =138.46 ± 3.97 µg/mL) and promoted glucose uptake across plasma membranes of yeast cells in a manner comparable to that of metformin. Interestingly, the extract demonstrated in vivo blood glucose normalization effects with concomitant increased activities of antioxidant parameters (superoxide dismutase (SOD), catalase, and reduced glutathione (GSH)) while decreasing malondialdehyde (MDA) levels when compared to untreated rats. Similarly, serum biochemical alterations, and tissues (liver, kidney, and pancreases) histopathological aberrations in untreated rats with STZ-induced diabetes were attenuated by treatment with the EESTL. Biometabolite characterization of the extract identified gallic acid (45.81 ppm), catechin (1.18 ppm), p-coumaric acid (1.43e−1 ppm), DL-proline 5-oxo-methyl ester (9.16 %, retention time (RT): 8.57 min), salicylic acid (3.26% and 7.61 min), and butylated hydroxytoluene (4.75%, RT: 10.18 min) as the major polyphenolic compounds in the plant extract. In conclusion, our study provides preclinical evidence of the antioxidant properties and oxidative stress-preventing role of S. torvum in STZ-dosed diabetic rats. Taken together, the EESTL represents a reserve of bioactive metabolites for managing diabetes and associated complications.http://www.sciencedirect.com/science/article/pii/S0753332222009945Solanum torvum SwartzOxidative stressDiabetes mellitusOrgan histopathologyAntioxidant |
| spellingShingle | Saidu Sani Bashir Lawal Jerius N. Ejeje Tawakalitu B. Aliu Amos S. Onikanni Onwe O. Uchewa Joy C. Ovoh Faith U. Ekpa Chikezie D. Ozoagu Tochukwu S. Akuma Success C. Onyeji Amara Obialor Saqer S. Alotaibi Sarah M. Albogami Michel De Waard Gaber El-Saber Batiha Tse Hung Huang Alexander T.H. Wu Biochemical and tissue physiopathological evaluation of the preclinical efficacy of Solanum torvum Swartz leaves for treating oxidative impairment in rats administered a β-cell-toxicant (STZ) Biomedicine & Pharmacotherapy Solanum torvum Swartz Oxidative stress Diabetes mellitus Organ histopathology Antioxidant |
| title | Biochemical and tissue physiopathological evaluation of the preclinical efficacy of Solanum torvum Swartz leaves for treating oxidative impairment in rats administered a β-cell-toxicant (STZ) |
| title_full | Biochemical and tissue physiopathological evaluation of the preclinical efficacy of Solanum torvum Swartz leaves for treating oxidative impairment in rats administered a β-cell-toxicant (STZ) |
| title_fullStr | Biochemical and tissue physiopathological evaluation of the preclinical efficacy of Solanum torvum Swartz leaves for treating oxidative impairment in rats administered a β-cell-toxicant (STZ) |
| title_full_unstemmed | Biochemical and tissue physiopathological evaluation of the preclinical efficacy of Solanum torvum Swartz leaves for treating oxidative impairment in rats administered a β-cell-toxicant (STZ) |
| title_short | Biochemical and tissue physiopathological evaluation of the preclinical efficacy of Solanum torvum Swartz leaves for treating oxidative impairment in rats administered a β-cell-toxicant (STZ) |
| title_sort | biochemical and tissue physiopathological evaluation of the preclinical efficacy of solanum torvum swartz leaves for treating oxidative impairment in rats administered a β cell toxicant stz |
| topic | Solanum torvum Swartz Oxidative stress Diabetes mellitus Organ histopathology Antioxidant |
| url | http://www.sciencedirect.com/science/article/pii/S0753332222009945 |
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