Potential of Limestonevirus Bacteriophages for Ecological Control of <i>Dickeya solani</i> Causing Bacterial Potato Blackleg
Pectinolytic bacteria of the family <i>Enterobacteriaceae</i>, specifically <i>Dickeya solani</i>, are known to cause potato blackleg. This study aimed to evaluate the effectiveness of a mixture of two bacteriophages from the genus <i>Limestonevirus</i> in control...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
|
| Series: | Agriculture |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0472/14/3/497 |
| _version_ | 1797242450526339072 |
|---|---|
| author | Martin Kmoch Josef Vacek Věra Loubová Karel Petrzik Sára Brázdová Rudolf Ševčík |
| author_facet | Martin Kmoch Josef Vacek Věra Loubová Karel Petrzik Sára Brázdová Rudolf Ševčík |
| author_sort | Martin Kmoch |
| collection | DOAJ |
| description | Pectinolytic bacteria of the family <i>Enterobacteriaceae</i>, specifically <i>Dickeya solani</i>, are known to cause potato blackleg. This study aimed to evaluate the effectiveness of a mixture of two bacteriophages from the genus <i>Limestonevirus</i> in controlling <i>Dickeya solani</i> in both greenhouse and field trials. The potential of bacteriophages for ecological potato control was also assessed. The phages φDs3CZ and φDs20CZ were isolated from soil in the Czech Republic between 2019 and 2021. They were applied preventively and curatively as a solution on artificially wounded and inoculated seed tubers immediately prior to planting. The phage-treated variant showed a highly significant reduction in the extent of <i>D. solani</i> infection compared to the untreated control in both the greenhouse and field trial. The effect of the phages depended on the concentration of the solution, the rate of tuber injury, and the sequence of application. When applied preventively, the phages caused a significantly higher reduction in the rate of blackleg symptoms (86.7% and/or 87.1%) compared to the curative application (54.6 and/or 36.6%). Phages φDs3CZ and φDs20CZ showed potential for use in biological potato control against <i>Dickeya solani</i>. |
| first_indexed | 2024-04-24T18:39:25Z |
| format | Article |
| id | doaj.art-f683080e28414f6cba70686fd20e3c0f |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-04-24T18:39:25Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-f683080e28414f6cba70686fd20e3c0f2024-03-27T13:16:13ZengMDPI AGAgriculture2077-04722024-03-0114349710.3390/agriculture14030497Potential of Limestonevirus Bacteriophages for Ecological Control of <i>Dickeya solani</i> Causing Bacterial Potato BlacklegMartin Kmoch0Josef Vacek1Věra Loubová2Karel Petrzik3Sára Brázdová4Rudolf Ševčík5Laboratory of Virology, Department of Genetic Resources, Potato Research Institute Havlíčkův Brod, Dobrovského 2366, 580 01 Havlíčkův Brod, Czech RepublicDepartment of Growing Technologies, Potato Research Institute Havlíčkův Brod, Dobrovského 2366, 580 01 Havlíčkův Brod, Czech RepublicLaboratory of Virology, Department of Genetic Resources, Potato Research Institute Havlíčkův Brod, Dobrovského 2366, 580 01 Havlíčkův Brod, Czech RepublicInstitute of Molecular Biology, Biology Centre of the Czech Academy of Sciences, Branišovská 1160/31, 370 05 České Budějovice, Czech RepublicInstitute of Molecular Biology, Biology Centre of the Czech Academy of Sciences, Branišovská 1160/31, 370 05 České Budějovice, Czech RepublicInstitute of Food Preservation, Faculty of Food and Biochemical Technology, University of Chemistry and Technology (VŠCHT), Technická 3, Prague 6—Dejvice, 166 28 Prague, Czech RepublicPectinolytic bacteria of the family <i>Enterobacteriaceae</i>, specifically <i>Dickeya solani</i>, are known to cause potato blackleg. This study aimed to evaluate the effectiveness of a mixture of two bacteriophages from the genus <i>Limestonevirus</i> in controlling <i>Dickeya solani</i> in both greenhouse and field trials. The potential of bacteriophages for ecological potato control was also assessed. The phages φDs3CZ and φDs20CZ were isolated from soil in the Czech Republic between 2019 and 2021. They were applied preventively and curatively as a solution on artificially wounded and inoculated seed tubers immediately prior to planting. The phage-treated variant showed a highly significant reduction in the extent of <i>D. solani</i> infection compared to the untreated control in both the greenhouse and field trial. The effect of the phages depended on the concentration of the solution, the rate of tuber injury, and the sequence of application. When applied preventively, the phages caused a significantly higher reduction in the rate of blackleg symptoms (86.7% and/or 87.1%) compared to the curative application (54.6 and/or 36.6%). Phages φDs3CZ and φDs20CZ showed potential for use in biological potato control against <i>Dickeya solani</i>.https://www.mdpi.com/2077-0472/14/3/497<i>Enterobacteriaceae</i><i>Solanum tuberosum</i><i>Limestonevirus</i>blacklegbiological control |
| spellingShingle | Martin Kmoch Josef Vacek Věra Loubová Karel Petrzik Sára Brázdová Rudolf Ševčík Potential of Limestonevirus Bacteriophages for Ecological Control of <i>Dickeya solani</i> Causing Bacterial Potato Blackleg Agriculture <i>Enterobacteriaceae</i> <i>Solanum tuberosum</i> <i>Limestonevirus</i> blackleg biological control |
| title | Potential of Limestonevirus Bacteriophages for Ecological Control of <i>Dickeya solani</i> Causing Bacterial Potato Blackleg |
| title_full | Potential of Limestonevirus Bacteriophages for Ecological Control of <i>Dickeya solani</i> Causing Bacterial Potato Blackleg |
| title_fullStr | Potential of Limestonevirus Bacteriophages for Ecological Control of <i>Dickeya solani</i> Causing Bacterial Potato Blackleg |
| title_full_unstemmed | Potential of Limestonevirus Bacteriophages for Ecological Control of <i>Dickeya solani</i> Causing Bacterial Potato Blackleg |
| title_short | Potential of Limestonevirus Bacteriophages for Ecological Control of <i>Dickeya solani</i> Causing Bacterial Potato Blackleg |
| title_sort | potential of limestonevirus bacteriophages for ecological control of i dickeya solani i causing bacterial potato blackleg |
| topic | <i>Enterobacteriaceae</i> <i>Solanum tuberosum</i> <i>Limestonevirus</i> blackleg biological control |
| url | https://www.mdpi.com/2077-0472/14/3/497 |
| work_keys_str_mv | AT martinkmoch potentialoflimestonevirusbacteriophagesforecologicalcontrolofidickeyasolaniicausingbacterialpotatoblackleg AT josefvacek potentialoflimestonevirusbacteriophagesforecologicalcontrolofidickeyasolaniicausingbacterialpotatoblackleg AT veraloubova potentialoflimestonevirusbacteriophagesforecologicalcontrolofidickeyasolaniicausingbacterialpotatoblackleg AT karelpetrzik potentialoflimestonevirusbacteriophagesforecologicalcontrolofidickeyasolaniicausingbacterialpotatoblackleg AT sarabrazdova potentialoflimestonevirusbacteriophagesforecologicalcontrolofidickeyasolaniicausingbacterialpotatoblackleg AT rudolfsevcik potentialoflimestonevirusbacteriophagesforecologicalcontrolofidickeyasolaniicausingbacterialpotatoblackleg |