Breaking the Chain of Infection: Dental Unit Water Quality Control

Introduction: The air–water syringes, ultrasonic scalers, high speed air turbine handpieces are connected to dental units by a network of small-bore plastic tubes through which water and air travel to activate or cool the instruments and it had been shown that this system is extensively contamin...

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Main Authors: Amrita Pawar, Sandeep Garg, Sonia Mehta, Rajat Dang
Format: Article
Language:English
Published: JCDR Research and Publications Private Limited 2016-07-01
Series:Journal of Clinical and Diagnostic Research
Subjects:
Online Access:https://jcdr.net/articles/PDF/8196/19070_CE(Ra1)_F(AK)_PF1(ACAK)_PFA(AK)_PF2(PAG).pdf
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author Amrita Pawar
Sandeep Garg
Sonia Mehta
Rajat Dang
author_facet Amrita Pawar
Sandeep Garg
Sonia Mehta
Rajat Dang
author_sort Amrita Pawar
collection DOAJ
description Introduction: The air–water syringes, ultrasonic scalers, high speed air turbine handpieces are connected to dental units by a network of small-bore plastic tubes through which water and air travel to activate or cool the instruments and it had been shown that this system is extensively contaminated with microbial biofilms and pose a potential risk of infection for patients as well as dental professionals. Aim: To evaluate and compare the efficacy of various disinfectants in reducing the microbial colony count in water derived from Dental Unit Waterlines. Materials and Methods: Five random dental units were selected and samples were collected before and after intervention with 5 disinfectants (0.02% H2 O2 continuously, 0.02% H2 O2 continuously with shock treatment with 0.25% H2 O2 weekly, 0.12% Chlorohexidine and 12% Ethanol overnight, 1:50 Original Listerine overnight, 2% Sodium Perborate and 2% EDTA 5 minutes in morning) using different disinfection methods for 4 weeks. Samples were cultured on Reasoner’s 2A (R2A) agar for microbial counting. Results: Results were recorded as Colony forming units/ml (cfu/ ml) and were evaluated statistically. Results showed that all the dental unit waterlines were heavily contaminated with microbes before any intervention. After 1 day of disinfection regime the counts reduced significantly and showed progressive reduction in consecutive weeks. Goals set by ADA & CDC were ultimately achieved at the end of 4 weeks. Conclusion: All the disinfectants were equally effective in reducing the microbial colony count of DUWLs, irrespective of their concentration and method of disinfection.
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spelling doaj.art-f683d94496964b1da19c9beee84926862022-12-21T20:09:22ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-07-01107ZC80ZC8410.7860/JCDR/2016/19070.8196Breaking the Chain of Infection: Dental Unit Water Quality ControlAmrita Pawar0Sandeep Garg1Sonia Mehta2Rajat Dang3Senior Lecturer, Department of Prosthodontics, ITS College of Dental Sciences & Research, Muradnagar, Ghaziabad, UP, India.Professor and Head, Department of Prosthodontics, MM College of Dental Sciences & Research, Mullana, Ambala, Haryana, India.Associate Professor, Department of Microbiology, MM College of Medical Sciences & Research, Mullana, Ambala, Haryana, India.Professor, Department of Prosthodontics, MM College of Dental Sciences & Research, Mullana, Ambala, Haryana, India.Introduction: The air–water syringes, ultrasonic scalers, high speed air turbine handpieces are connected to dental units by a network of small-bore plastic tubes through which water and air travel to activate or cool the instruments and it had been shown that this system is extensively contaminated with microbial biofilms and pose a potential risk of infection for patients as well as dental professionals. Aim: To evaluate and compare the efficacy of various disinfectants in reducing the microbial colony count in water derived from Dental Unit Waterlines. Materials and Methods: Five random dental units were selected and samples were collected before and after intervention with 5 disinfectants (0.02% H2 O2 continuously, 0.02% H2 O2 continuously with shock treatment with 0.25% H2 O2 weekly, 0.12% Chlorohexidine and 12% Ethanol overnight, 1:50 Original Listerine overnight, 2% Sodium Perborate and 2% EDTA 5 minutes in morning) using different disinfection methods for 4 weeks. Samples were cultured on Reasoner’s 2A (R2A) agar for microbial counting. Results: Results were recorded as Colony forming units/ml (cfu/ ml) and were evaluated statistically. Results showed that all the dental unit waterlines were heavily contaminated with microbes before any intervention. After 1 day of disinfection regime the counts reduced significantly and showed progressive reduction in consecutive weeks. Goals set by ADA & CDC were ultimately achieved at the end of 4 weeks. Conclusion: All the disinfectants were equally effective in reducing the microbial colony count of DUWLs, irrespective of their concentration and method of disinfection.https://jcdr.net/articles/PDF/8196/19070_CE(Ra1)_F(AK)_PF1(ACAK)_PFA(AK)_PF2(PAG).pdfdental unit waterlinesdisinfectionhydrogen peroxideinfection control
spellingShingle Amrita Pawar
Sandeep Garg
Sonia Mehta
Rajat Dang
Breaking the Chain of Infection: Dental Unit Water Quality Control
Journal of Clinical and Diagnostic Research
dental unit waterlines
disinfection
hydrogen peroxide
infection control
title Breaking the Chain of Infection: Dental Unit Water Quality Control
title_full Breaking the Chain of Infection: Dental Unit Water Quality Control
title_fullStr Breaking the Chain of Infection: Dental Unit Water Quality Control
title_full_unstemmed Breaking the Chain of Infection: Dental Unit Water Quality Control
title_short Breaking the Chain of Infection: Dental Unit Water Quality Control
title_sort breaking the chain of infection dental unit water quality control
topic dental unit waterlines
disinfection
hydrogen peroxide
infection control
url https://jcdr.net/articles/PDF/8196/19070_CE(Ra1)_F(AK)_PF1(ACAK)_PFA(AK)_PF2(PAG).pdf
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