Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.

Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discrim...

Full description

Bibliographic Details
Main Authors: Pavan Ramdya, Thomas Schaffter, Dario Floreano, Richard Benton
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3492344?pdf=render
_version_ 1819039253966880768
author Pavan Ramdya
Thomas Schaffter
Dario Floreano
Richard Benton
author_facet Pavan Ramdya
Thomas Schaffter
Dario Floreano
Richard Benton
author_sort Pavan Ramdya
collection DOAJ
description Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila.
first_indexed 2024-12-21T08:50:17Z
format Article
id doaj.art-f6842967b67c433ba6527f4ba2f0c6ce
institution Directory Open Access Journal
issn 1932-6203
language English
last_indexed 2024-12-21T08:50:17Z
publishDate 2012-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS ONE
spelling doaj.art-f6842967b67c433ba6527f4ba2f0c6ce2022-12-21T19:09:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e4838110.1371/journal.pone.0048381Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.Pavan RamdyaThomas SchaffterDario FloreanoRichard BentonDistinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila.http://europepmc.org/articles/PMC3492344?pdf=render
spellingShingle Pavan Ramdya
Thomas Schaffter
Dario Floreano
Richard Benton
Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.
PLoS ONE
title Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.
title_full Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.
title_fullStr Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.
title_full_unstemmed Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.
title_short Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.
title_sort fluorescence behavioral imaging fbi tracks identity in heterogeneous groups of drosophila
url http://europepmc.org/articles/PMC3492344?pdf=render
work_keys_str_mv AT pavanramdya fluorescencebehavioralimagingfbitracksidentityinheterogeneousgroupsofdrosophila
AT thomasschaffter fluorescencebehavioralimagingfbitracksidentityinheterogeneousgroupsofdrosophila
AT dariofloreano fluorescencebehavioralimagingfbitracksidentityinheterogeneousgroupsofdrosophila
AT richardbenton fluorescencebehavioralimagingfbitracksidentityinheterogeneousgroupsofdrosophila