Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.
Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discrim...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3492344?pdf=render |
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author | Pavan Ramdya Thomas Schaffter Dario Floreano Richard Benton |
author_facet | Pavan Ramdya Thomas Schaffter Dario Floreano Richard Benton |
author_sort | Pavan Ramdya |
collection | DOAJ |
description | Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila. |
first_indexed | 2024-12-21T08:50:17Z |
format | Article |
id | doaj.art-f6842967b67c433ba6527f4ba2f0c6ce |
institution | Directory Open Access Journal |
issn | 1932-6203 |
language | English |
last_indexed | 2024-12-21T08:50:17Z |
publishDate | 2012-01-01 |
publisher | Public Library of Science (PLoS) |
record_format | Article |
series | PLoS ONE |
spelling | doaj.art-f6842967b67c433ba6527f4ba2f0c6ce2022-12-21T19:09:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e4838110.1371/journal.pone.0048381Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.Pavan RamdyaThomas SchaffterDario FloreanoRichard BentonDistinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila.http://europepmc.org/articles/PMC3492344?pdf=render |
spellingShingle | Pavan Ramdya Thomas Schaffter Dario Floreano Richard Benton Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila. PLoS ONE |
title | Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila. |
title_full | Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila. |
title_fullStr | Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila. |
title_full_unstemmed | Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila. |
title_short | Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila. |
title_sort | fluorescence behavioral imaging fbi tracks identity in heterogeneous groups of drosophila |
url | http://europepmc.org/articles/PMC3492344?pdf=render |
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