Identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence-activated cell sorting

Dietary glycans are a major driver of the human gut microbiota composition. Here, the authors apply next-generation metabolic labeling coupled with fluorescence-activated cell sorting to identify and isolate gut bacteria consumers of dietary glycans in human stool samples, linking bacteria to the gl...

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Main Authors: Lharbi Dridi, Fernando Altamura, Emmanuel Gonzalez, Olivia Lui, Ryszard Kubinski, Reilly Pidgeon, Adrian Montagut, Jasmine Chong, Jianguo Xia, Corinne F. Maurice, Bastien Castagner
Format: Article
Language:English
Published: Nature Portfolio 2023-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-023-36365-8
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author Lharbi Dridi
Fernando Altamura
Emmanuel Gonzalez
Olivia Lui
Ryszard Kubinski
Reilly Pidgeon
Adrian Montagut
Jasmine Chong
Jianguo Xia
Corinne F. Maurice
Bastien Castagner
author_facet Lharbi Dridi
Fernando Altamura
Emmanuel Gonzalez
Olivia Lui
Ryszard Kubinski
Reilly Pidgeon
Adrian Montagut
Jasmine Chong
Jianguo Xia
Corinne F. Maurice
Bastien Castagner
author_sort Lharbi Dridi
collection DOAJ
description Dietary glycans are a major driver of the human gut microbiota composition. Here, the authors apply next-generation metabolic labeling coupled with fluorescence-activated cell sorting to identify and isolate gut bacteria consumers of dietary glycans in human stool samples, linking bacteria to the glycans they consume.
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spelling doaj.art-f6847d551c734c8c8c540d20945b6a702023-02-12T12:15:47ZengNature PortfolioNature Communications2041-17232023-02-0114111310.1038/s41467-023-36365-8Identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence-activated cell sortingLharbi Dridi0Fernando Altamura1Emmanuel Gonzalez2Olivia Lui3Ryszard Kubinski4Reilly Pidgeon5Adrian Montagut6Jasmine Chong7Jianguo Xia8Corinne F. Maurice9Bastien Castagner10Department of Pharmacology & Therapeutics, McGill UniversityDepartment of Pharmacology & Therapeutics, McGill UniversityCanadian Centre for Computational Genomics, McGill Genome CenterDepartment of Pharmacology & Therapeutics, McGill UniversityDepartment of Pharmacology & Therapeutics, McGill UniversityDepartment of Pharmacology & Therapeutics, McGill UniversityDepartment of Pharmacology & Therapeutics, McGill UniversityInstitute of Parasitology, McGill UniversityInstitute of Parasitology, McGill UniversityMcGill Centre for Microbiome Research, McGill UniversityDepartment of Pharmacology & Therapeutics, McGill UniversityDietary glycans are a major driver of the human gut microbiota composition. Here, the authors apply next-generation metabolic labeling coupled with fluorescence-activated cell sorting to identify and isolate gut bacteria consumers of dietary glycans in human stool samples, linking bacteria to the glycans they consume.https://doi.org/10.1038/s41467-023-36365-8
spellingShingle Lharbi Dridi
Fernando Altamura
Emmanuel Gonzalez
Olivia Lui
Ryszard Kubinski
Reilly Pidgeon
Adrian Montagut
Jasmine Chong
Jianguo Xia
Corinne F. Maurice
Bastien Castagner
Identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence-activated cell sorting
Nature Communications
title Identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence-activated cell sorting
title_full Identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence-activated cell sorting
title_fullStr Identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence-activated cell sorting
title_full_unstemmed Identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence-activated cell sorting
title_short Identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence-activated cell sorting
title_sort identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence activated cell sorting
url https://doi.org/10.1038/s41467-023-36365-8
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