Ezrin promotes esophageal squamous cell carcinoma progression via the Hippo signaling pathway
The aim of this study was to analyze the role of Ezrin in esophageal squamous cell carcinoma (ESCC) and investigate potential therapeutic targets for ESCC by interfering with Ezrin expression. Bioinformatics analysis revealed that Ezrin expression differed significantly among patients with different...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-09-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2022-0743 |
| _version_ | 1797668807361167360 |
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| author | Ma Lijuan Liu Li Ji Min Su Liping Guan Yaling Xiao Jinling Pu Hongwei |
| author_facet | Ma Lijuan Liu Li Ji Min Su Liping Guan Yaling Xiao Jinling Pu Hongwei |
| author_sort | Ma Lijuan |
| collection | DOAJ |
| description | The aim of this study was to analyze the role of Ezrin in esophageal squamous cell carcinoma (ESCC) and investigate potential therapeutic targets for ESCC by interfering with Ezrin expression. Bioinformatics analysis revealed that Ezrin expression differed significantly among patients with different clinical stage ESCC. Moreover, there was a significant correlation between Ezrin and yes-associated protein/connective tissue growth factor (YAP1/CTGF) levels in esophageal cancer. Sixty paraffin-embedded ESCC tissue samples were examined and Ezrin and YAP1/CTGF levels were determined using immunohistochemistry. The positive expression rates of Ezrin and YAP1/CTGF were significantly lower in adjacent tissues than in ESCC tissues. Furthermore, knockdown of Ezrin expression inhibited colony formation and reduced cell migration and invasion. Compared with control ESCC cells, protein expression levels of YAP1 and CTGF were significantly downregulated in cells with Ezrin knocked down. We conclude that Ezrin may be involved in ESCC progression through the Hippo signaling pathway. |
| first_indexed | 2024-03-11T20:34:50Z |
| format | Article |
| id | doaj.art-f684933c26764a8c9b3a79412a983452 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-03-11T20:34:50Z |
| publishDate | 2023-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-f684933c26764a8c9b3a79412a9834522023-10-02T07:37:25ZengDe GruyterOpen Life Sciences2391-54122023-09-011812094910.1515/biol-2022-0743Ezrin promotes esophageal squamous cell carcinoma progression via the Hippo signaling pathwayMa Lijuan0Liu Li1Ji Min2Su Liping3Guan Yaling4Xiao Jinling5Pu Hongwei6State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Physiology, School of Basic Medical Sciences, Xinjiang Medical University, No. 567, Shangde North Road, Urumqi830017, Xinjiang, ChinaDepartment of Pathology, First Affiliated Hospital, Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi830054, Xinjiang, ChinaDepartment of Pathology, First Affiliated Hospital, Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi830054, Xinjiang, ChinaDepartment of Pathology, First Affiliated Hospital, Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi830054, Xinjiang, ChinaDepartment of Pathology, School of Basic Medical Sciences, Xinjiang Medical University, No. 567, Shangde North Road, Urumqi830017, Xinjiang, ChinaDepartment of Pathology, School of Basic Medical Sciences, Xinjiang Medical University, No. 567, Shangde North Road, Urumqi830017, Xinjiang, ChinaDepartment of Discipline Construction, First Affiliated Hospital, Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi830054, Xinjiang, ChinaThe aim of this study was to analyze the role of Ezrin in esophageal squamous cell carcinoma (ESCC) and investigate potential therapeutic targets for ESCC by interfering with Ezrin expression. Bioinformatics analysis revealed that Ezrin expression differed significantly among patients with different clinical stage ESCC. Moreover, there was a significant correlation between Ezrin and yes-associated protein/connective tissue growth factor (YAP1/CTGF) levels in esophageal cancer. Sixty paraffin-embedded ESCC tissue samples were examined and Ezrin and YAP1/CTGF levels were determined using immunohistochemistry. The positive expression rates of Ezrin and YAP1/CTGF were significantly lower in adjacent tissues than in ESCC tissues. Furthermore, knockdown of Ezrin expression inhibited colony formation and reduced cell migration and invasion. Compared with control ESCC cells, protein expression levels of YAP1 and CTGF were significantly downregulated in cells with Ezrin knocked down. We conclude that Ezrin may be involved in ESCC progression through the Hippo signaling pathway.https://doi.org/10.1515/biol-2022-0743esophageal squamous cell carcinomaezrinyes-associated proteinhippo signaling pathwayconnective tissue growth factor |
| spellingShingle | Ma Lijuan Liu Li Ji Min Su Liping Guan Yaling Xiao Jinling Pu Hongwei Ezrin promotes esophageal squamous cell carcinoma progression via the Hippo signaling pathway Open Life Sciences esophageal squamous cell carcinoma ezrin yes-associated protein hippo signaling pathway connective tissue growth factor |
| title | Ezrin promotes esophageal squamous cell carcinoma progression via the Hippo signaling pathway |
| title_full | Ezrin promotes esophageal squamous cell carcinoma progression via the Hippo signaling pathway |
| title_fullStr | Ezrin promotes esophageal squamous cell carcinoma progression via the Hippo signaling pathway |
| title_full_unstemmed | Ezrin promotes esophageal squamous cell carcinoma progression via the Hippo signaling pathway |
| title_short | Ezrin promotes esophageal squamous cell carcinoma progression via the Hippo signaling pathway |
| title_sort | ezrin promotes esophageal squamous cell carcinoma progression via the hippo signaling pathway |
| topic | esophageal squamous cell carcinoma ezrin yes-associated protein hippo signaling pathway connective tissue growth factor |
| url | https://doi.org/10.1515/biol-2022-0743 |
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