AFLP genomic characterization of <em>Stevia rebaudiana</em> Bertoni cultivated in department of Antioquia (Colombia)
Stevia rebaudiana Bertoni, Asteraceae family (2n = 22), is a plant native from Paraguay, it has in leaves tissues a high production of some substances with strong edulcorant power, getting to be 320 sweet times but that the sugar. Stevia was introduced, in Department of Antioquia, Colombia, at diffe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universidad Central Marta Abreu de Las Villas
2007-04-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/364 |
| _version_ | 1818903314889179136 |
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| author | Diego Martínez Luis Rojas Aura Urreal Elio Jiménez Zulma Monsalve F |
| author_facet | Diego Martínez Luis Rojas Aura Urreal Elio Jiménez Zulma Monsalve F |
| author_sort | Diego Martínez |
| collection | DOAJ |
| description | Stevia rebaudiana Bertoni, Asteraceae family (2n = 22), is a plant native from Paraguay, it has in leaves tissues a high production of some substances with strong edulcorant power, getting to be 320 sweet times but that the sugar. Stevia was introduced, in Department of Antioquia, Colombia, at different times from diverse places. Its cultivation has been spread in the Department, additionally is an alternative for illicit cultivated crops substitution. AFLP marker were generated from genomic DNA of two reference varieties (‘Morita’ and ‘Bertoni’) and samples from fields crops located in different regions of Department of Antioquia. Ten AFLP primer combinations revealed 923 bands and 39.22% polymorphism. Dice coefficient similarity analysis show differences between both ‘Morita’ and ‘Bertoni’ varieties. Primer combination EcoRI-AGG/ Tru 9I-ATG, was able to identify every of two cultivars, and established two groups for Department of Antioquia Stevia varieties. Samples from Támesis, Andes, Jericó, San José del Nus and Santa Fe de Antioquia localities were grouped within ‘Morita’ cultivar and samples from Fredonia, Cisneros localities and University of Antioquia laboratories were grouped within ‘Bertoni’ variety.
Key words: ‘Bertoni’, molecular marker, ‘Morita’ |
| first_indexed | 2024-12-19T20:49:35Z |
| format | Article |
| id | doaj.art-f684f8be093642cdbd21f0936c6e79fb |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-19T20:49:35Z |
| publishDate | 2007-04-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-f684f8be093642cdbd21f0936c6e79fb2022-12-21T20:06:09ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472007-04-0172337AFLP genomic characterization of <em>Stevia rebaudiana</em> Bertoni cultivated in department of Antioquia (Colombia)Diego MartínezLuis RojasAura UrrealElio JiménezZulma Monsalve FStevia rebaudiana Bertoni, Asteraceae family (2n = 22), is a plant native from Paraguay, it has in leaves tissues a high production of some substances with strong edulcorant power, getting to be 320 sweet times but that the sugar. Stevia was introduced, in Department of Antioquia, Colombia, at different times from diverse places. Its cultivation has been spread in the Department, additionally is an alternative for illicit cultivated crops substitution. AFLP marker were generated from genomic DNA of two reference varieties (‘Morita’ and ‘Bertoni’) and samples from fields crops located in different regions of Department of Antioquia. Ten AFLP primer combinations revealed 923 bands and 39.22% polymorphism. Dice coefficient similarity analysis show differences between both ‘Morita’ and ‘Bertoni’ varieties. Primer combination EcoRI-AGG/ Tru 9I-ATG, was able to identify every of two cultivars, and established two groups for Department of Antioquia Stevia varieties. Samples from Támesis, Andes, Jericó, San José del Nus and Santa Fe de Antioquia localities were grouped within ‘Morita’ cultivar and samples from Fredonia, Cisneros localities and University of Antioquia laboratories were grouped within ‘Bertoni’ variety. Key words: ‘Bertoni’, molecular marker, ‘Morita’https://revista.ibp.co.cu/index.php/BV/article/view/364 |
| spellingShingle | Diego Martínez Luis Rojas Aura Urreal Elio Jiménez Zulma Monsalve F AFLP genomic characterization of <em>Stevia rebaudiana</em> Bertoni cultivated in department of Antioquia (Colombia) Biotecnología Vegetal |
| title | AFLP genomic characterization of <em>Stevia rebaudiana</em> Bertoni cultivated in department of Antioquia (Colombia) |
| title_full | AFLP genomic characterization of <em>Stevia rebaudiana</em> Bertoni cultivated in department of Antioquia (Colombia) |
| title_fullStr | AFLP genomic characterization of <em>Stevia rebaudiana</em> Bertoni cultivated in department of Antioquia (Colombia) |
| title_full_unstemmed | AFLP genomic characterization of <em>Stevia rebaudiana</em> Bertoni cultivated in department of Antioquia (Colombia) |
| title_short | AFLP genomic characterization of <em>Stevia rebaudiana</em> Bertoni cultivated in department of Antioquia (Colombia) |
| title_sort | aflp genomic characterization of em stevia rebaudiana em bertoni cultivated in department of antioquia colombia |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/364 |
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