Polylactic Acid-Based Patterned Matrixes for Site-Specific Delivery of Neuropeptides On-Demand: Functional NGF Effects on Human Neuronal Cells
The patterned microchamber arrays based on biocompatible polymers are a versatile cargo delivery system for drug storage and site-/time-specific drug release on demand. However, functional evidence of their action on nerve cells, in particular their potential for enabling patterned neuronal morphoge...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00497/full |
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| author | Olga A. Sindeeva Olga A. Sindeeva Olga Kopach Maxim A. Kurochkin Andrei Sapelkin David J. Gould Dmitri A. Rusakov Gleb B. Sukhorukov Gleb B. Sukhorukov Gleb B. Sukhorukov |
| author_facet | Olga A. Sindeeva Olga A. Sindeeva Olga Kopach Maxim A. Kurochkin Andrei Sapelkin David J. Gould Dmitri A. Rusakov Gleb B. Sukhorukov Gleb B. Sukhorukov Gleb B. Sukhorukov |
| author_sort | Olga A. Sindeeva |
| collection | DOAJ |
| description | The patterned microchamber arrays based on biocompatible polymers are a versatile cargo delivery system for drug storage and site-/time-specific drug release on demand. However, functional evidence of their action on nerve cells, in particular their potential for enabling patterned neuronal morphogenesis, remains unclear. Recently, we have established that the polylactic acid (PLA)-based microchamber arrays are biocompatible with human cells of neuronal phenotype and provide safe loading for hydrophilic substances of low molecular weight, with successive site-specific cargo release on-demand to trigger local cell responses. Here, we load the nerve growth factor (NGF) inside microchambers and grow N2A cells on the surface of patterned microchamber arrays. We find that the neurite outgrowth in local N2A cells can be preferentially directed towards opened microchambers (upon-specific NGF release). These observations suggest the PLA-microchambers can be an efficient drug delivery system for the site-specific delivery of neuropeptides on-demand, potentially suitable for the migratory or axonal guidance of human nerve cells. |
| first_indexed | 2024-04-13T15:29:26Z |
| format | Article |
| id | doaj.art-f685e079812c43d8a6c012ab5e0c7f4c |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-13T15:29:26Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-f685e079812c43d8a6c012ab5e0c7f4c2022-12-22T02:41:26ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-06-01810.3389/fbioe.2020.00497532061Polylactic Acid-Based Patterned Matrixes for Site-Specific Delivery of Neuropeptides On-Demand: Functional NGF Effects on Human Neuronal CellsOlga A. Sindeeva0Olga A. Sindeeva1Olga Kopach2Maxim A. Kurochkin3Andrei Sapelkin4David J. Gould5Dmitri A. Rusakov6Gleb B. Sukhorukov7Gleb B. Sukhorukov8Gleb B. Sukhorukov9Center for Neurobiology and Brain Restoration, Skolkovo Institute of Science and Technology, Moscow, RussiaRemote Controlled Theranostic Systems Lab, Department of Nanotechnology, Educational and Research Institute of Nanostructures and Biosystems, Saratov State University, Saratov, RussiaUCL Queen Square Institute of Neurology, University College London, London, United KingdomCenter for Photonics and Quantum Materials, Skolkovo Institute of Science and Technology, Moscow, RussiaSchool of Physics and Astronomy, Queen Mary University of London, London, United KingdomBiochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, United KingdomUCL Queen Square Institute of Neurology, University College London, London, United KingdomCenter for Neurobiology and Brain Restoration, Skolkovo Institute of Science and Technology, Moscow, RussiaSchool of Engineering and Material Science, Queen Mary University of London, London, United KingdomCenter of Biomedical Engineering, I.M. Sechenov First Moscow State Medical University, Moscow, RussiaThe patterned microchamber arrays based on biocompatible polymers are a versatile cargo delivery system for drug storage and site-/time-specific drug release on demand. However, functional evidence of their action on nerve cells, in particular their potential for enabling patterned neuronal morphogenesis, remains unclear. Recently, we have established that the polylactic acid (PLA)-based microchamber arrays are biocompatible with human cells of neuronal phenotype and provide safe loading for hydrophilic substances of low molecular weight, with successive site-specific cargo release on-demand to trigger local cell responses. Here, we load the nerve growth factor (NGF) inside microchambers and grow N2A cells on the surface of patterned microchamber arrays. We find that the neurite outgrowth in local N2A cells can be preferentially directed towards opened microchambers (upon-specific NGF release). These observations suggest the PLA-microchambers can be an efficient drug delivery system for the site-specific delivery of neuropeptides on-demand, potentially suitable for the migratory or axonal guidance of human nerve cells.https://www.frontiersin.org/article/10.3389/fbioe.2020.00497/fullpolylactic acid (PLA)patterned microchamber arraydrug delivery systemNGFhuman N2A cells |
| spellingShingle | Olga A. Sindeeva Olga A. Sindeeva Olga Kopach Maxim A. Kurochkin Andrei Sapelkin David J. Gould Dmitri A. Rusakov Gleb B. Sukhorukov Gleb B. Sukhorukov Gleb B. Sukhorukov Polylactic Acid-Based Patterned Matrixes for Site-Specific Delivery of Neuropeptides On-Demand: Functional NGF Effects on Human Neuronal Cells Frontiers in Bioengineering and Biotechnology polylactic acid (PLA) patterned microchamber array drug delivery system NGF human N2A cells |
| title | Polylactic Acid-Based Patterned Matrixes for Site-Specific Delivery of Neuropeptides On-Demand: Functional NGF Effects on Human Neuronal Cells |
| title_full | Polylactic Acid-Based Patterned Matrixes for Site-Specific Delivery of Neuropeptides On-Demand: Functional NGF Effects on Human Neuronal Cells |
| title_fullStr | Polylactic Acid-Based Patterned Matrixes for Site-Specific Delivery of Neuropeptides On-Demand: Functional NGF Effects on Human Neuronal Cells |
| title_full_unstemmed | Polylactic Acid-Based Patterned Matrixes for Site-Specific Delivery of Neuropeptides On-Demand: Functional NGF Effects on Human Neuronal Cells |
| title_short | Polylactic Acid-Based Patterned Matrixes for Site-Specific Delivery of Neuropeptides On-Demand: Functional NGF Effects on Human Neuronal Cells |
| title_sort | polylactic acid based patterned matrixes for site specific delivery of neuropeptides on demand functional ngf effects on human neuronal cells |
| topic | polylactic acid (PLA) patterned microchamber array drug delivery system NGF human N2A cells |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.00497/full |
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