Rapid, cost-effective, sensitive and quantitative detection of Acinetobacter baumannii from pneumonia patients

Background and Objectives: Pneumonia with Acinetobacter baumannii has a major therapeutic problem in health care settings. Decision to initiate correct antibiotic therapy requires rapid identification and quantification of organism. The aim of this study was to develop a rapid and sensitive method f...

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Main Authors: B Nomanpour, A Ghodousi, A Babaei, HR Abtahi, M Tabrizi, M Feizabadi
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2011-12-01
Series:Iranian Journal of Microbiology
Subjects:
Online Access:https://ijm.tums.ac.ir/index.php/ijm/article/view/108
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author B Nomanpour
A Ghodousi
A Babaei
HR Abtahi
M Tabrizi
M Feizabadi
author_facet B Nomanpour
A Ghodousi
A Babaei
HR Abtahi
M Tabrizi
M Feizabadi
author_sort B Nomanpour
collection DOAJ
description Background and Objectives: Pneumonia with Acinetobacter baumannii has a major therapeutic problem in health care settings. Decision to initiate correct antibiotic therapy requires rapid identification and quantification of organism. The aim of this study was to develop a rapid and sensitive method for direct detection of A. baumannii from respiratory specimens. Materials and Methods: A Taqman real time PCR based on the sequence of blaoxa-51 was designed and used for direct detection of A. baumannii from 361 respiratory specimens of patients with pneumonia. All specimens were checked by conventional bacteriology in parallel. Results: The new real time PCR could detect less than 200 cfu per ml of bacteria in specimens. There was agreement between the results of real time PCR and culture (Kappa value 1.0, p value < 0.001). The sensitivity, specificity and predictive values of real time PCR were 100%. The prevalence of A. baumannii in pneumonia patients was 10.53 % (n = 38). Poly-microbial infections were detected in 65.71% of specimens. Conclusion: Acinetobacter baumannii is the third causative agent in nosocomial pneumonia after Pseudomonas aeroginosa (16%) and Staphylococcus aureus (13%) at Tehran hospitals. We recommend that 104 CFU be the threshold for definition of infection with A. baumannii using real time PCR.
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spelling doaj.art-f68af4869a6248ada3c06ca18aa5ce162022-12-21T17:43:48ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472011-12-0134Rapid, cost-effective, sensitive and quantitative detection of Acinetobacter baumannii from pneumonia patientsB Nomanpour0A Ghodousi1A Babaei2HR Abtahi3M Tabrizi4M Feizabadi5Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Anesthesiology, Shaheed Rajaei Cardiovascular, Medical & Research Center, Vali- Asr Avenue Tehran, Iran.Department of Internal Medicine, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran AND Pediatric Infection Diseas Research Center, Tehran, University of Medical Science, Tehran, Iran.Background and Objectives: Pneumonia with Acinetobacter baumannii has a major therapeutic problem in health care settings. Decision to initiate correct antibiotic therapy requires rapid identification and quantification of organism. The aim of this study was to develop a rapid and sensitive method for direct detection of A. baumannii from respiratory specimens. Materials and Methods: A Taqman real time PCR based on the sequence of blaoxa-51 was designed and used for direct detection of A. baumannii from 361 respiratory specimens of patients with pneumonia. All specimens were checked by conventional bacteriology in parallel. Results: The new real time PCR could detect less than 200 cfu per ml of bacteria in specimens. There was agreement between the results of real time PCR and culture (Kappa value 1.0, p value < 0.001). The sensitivity, specificity and predictive values of real time PCR were 100%. The prevalence of A. baumannii in pneumonia patients was 10.53 % (n = 38). Poly-microbial infections were detected in 65.71% of specimens. Conclusion: Acinetobacter baumannii is the third causative agent in nosocomial pneumonia after Pseudomonas aeroginosa (16%) and Staphylococcus aureus (13%) at Tehran hospitals. We recommend that 104 CFU be the threshold for definition of infection with A. baumannii using real time PCR.https://ijm.tums.ac.ir/index.php/ijm/article/view/108HAPVAPAcinetobacter baumanniiReal time PCRPneumonia
spellingShingle B Nomanpour
A Ghodousi
A Babaei
HR Abtahi
M Tabrizi
M Feizabadi
Rapid, cost-effective, sensitive and quantitative detection of Acinetobacter baumannii from pneumonia patients
Iranian Journal of Microbiology
HAP
VAP
Acinetobacter baumannii
Real time PCR
Pneumonia
title Rapid, cost-effective, sensitive and quantitative detection of Acinetobacter baumannii from pneumonia patients
title_full Rapid, cost-effective, sensitive and quantitative detection of Acinetobacter baumannii from pneumonia patients
title_fullStr Rapid, cost-effective, sensitive and quantitative detection of Acinetobacter baumannii from pneumonia patients
title_full_unstemmed Rapid, cost-effective, sensitive and quantitative detection of Acinetobacter baumannii from pneumonia patients
title_short Rapid, cost-effective, sensitive and quantitative detection of Acinetobacter baumannii from pneumonia patients
title_sort rapid cost effective sensitive and quantitative detection of acinetobacter baumannii from pneumonia patients
topic HAP
VAP
Acinetobacter baumannii
Real time PCR
Pneumonia
url https://ijm.tums.ac.ir/index.php/ijm/article/view/108
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