Relationship between Promoter Hypermethylation of DNMT3A and DNMT3B genes and Endometrial Cancer
Aberrant DNA methylation is an epigenetic event that occurs by methyltransferases. DNMT3A and DNMT3B are responsible for de novo methylation that plays important roles in normal development and disease. A number of reports on methylation of various genes in endometrial cancer have been published, bu...
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| Format: | Article |
| Language: | English |
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University of Sistan and Baluchestan
2019-02-01
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| Series: | Journal of Epigenetics |
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| Online Access: | https://jep.usb.ac.ir/article_4476_0fad5f920205862869910d78d675fcfb.pdf |
| _version_ | 1797824946097881088 |
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| author | Masoumeh Omidali Neda Jabbara Golnaz Asaadi tehrani |
| author_facet | Masoumeh Omidali Neda Jabbara Golnaz Asaadi tehrani |
| author_sort | Masoumeh Omidali |
| collection | DOAJ |
| description | Aberrant DNA methylation is an epigenetic event that occurs by methyltransferases. DNMT3A and DNMT3B are responsible for de novo methylation that plays important roles in normal development and disease. A number of reports on methylation of various genes in endometrial cancer have been published, but most of these studies focused on tumor suppressor genes. In this study, we determined the promoter methylation pattern of DNMT3A and DNMT3B genes; also we analyzed correlations between methylation statuses with clinicopathological parameters. 28 patients and 22 healthy controls were studied. Isolation of genomic DNA from FFPE and peripheral blood was performed and Methylation-Specific PCR (MSP) was applied for analysis the promoter CpG methylation status of DNMT3A and DNMT3Bgenes in the studied population. A significant difference was found between the study groups and the presence of promoter CpG hypermethylation status in the DNMT3A (P=0.04) gene. Furthermore methylation status between tissue and blood samples of DNMT3A genewas not significant (p=0.78). Our results indicated correlation between age and menopausal state with DNMT3B promoter methylation, but there were no significant relationships between parameters such as tumor grade, type of tumor, amount of metastasis and myometrium invasion, furthermore diabetes (p = 0.01) and obesity (p = 0.027) were two important items in endometrial cancer incidence. In our study hypermethylation of DNMT3A gene was found as an important event in carcinogenesis of endometrial cancer. The inactivation epigenetic of methylation regulation genes is a common occurrence in many cancers, including endometrial cancer. |
| first_indexed | 2024-03-13T10:46:49Z |
| format | Article |
| id | doaj.art-f68c84cb24d94fbe84df891c89130f24 |
| institution | Directory Open Access Journal |
| issn | 2676-4350 |
| language | English |
| last_indexed | 2024-03-13T10:46:49Z |
| publishDate | 2019-02-01 |
| publisher | University of Sistan and Baluchestan |
| record_format | Article |
| series | Journal of Epigenetics |
| spelling | doaj.art-f68c84cb24d94fbe84df891c89130f242023-05-17T18:57:05ZengUniversity of Sistan and BaluchestanJournal of Epigenetics2676-43502019-02-0111192310.22111/jep.2019.27203.10054476Relationship between Promoter Hypermethylation of DNMT3A and DNMT3B genes and Endometrial CancerMasoumeh Omidali0Neda Jabbara1Golnaz Asaadi tehrani2MSc of Biology-Genetics, Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan, Iran.MSc of Biology-Genetics, Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan, Iran.Assistant Professor, Molecular Genetics Ph.D, Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan, Iran.Aberrant DNA methylation is an epigenetic event that occurs by methyltransferases. DNMT3A and DNMT3B are responsible for de novo methylation that plays important roles in normal development and disease. A number of reports on methylation of various genes in endometrial cancer have been published, but most of these studies focused on tumor suppressor genes. In this study, we determined the promoter methylation pattern of DNMT3A and DNMT3B genes; also we analyzed correlations between methylation statuses with clinicopathological parameters. 28 patients and 22 healthy controls were studied. Isolation of genomic DNA from FFPE and peripheral blood was performed and Methylation-Specific PCR (MSP) was applied for analysis the promoter CpG methylation status of DNMT3A and DNMT3Bgenes in the studied population. A significant difference was found between the study groups and the presence of promoter CpG hypermethylation status in the DNMT3A (P=0.04) gene. Furthermore methylation status between tissue and blood samples of DNMT3A genewas not significant (p=0.78). Our results indicated correlation between age and menopausal state with DNMT3B promoter methylation, but there were no significant relationships between parameters such as tumor grade, type of tumor, amount of metastasis and myometrium invasion, furthermore diabetes (p = 0.01) and obesity (p = 0.027) were two important items in endometrial cancer incidence. In our study hypermethylation of DNMT3A gene was found as an important event in carcinogenesis of endometrial cancer. The inactivation epigenetic of methylation regulation genes is a common occurrence in many cancers, including endometrial cancer.https://jep.usb.ac.ir/article_4476_0fad5f920205862869910d78d675fcfb.pdfendometrial cancerpromoter hypermethylationmspdnmt3adnmt3b |
| spellingShingle | Masoumeh Omidali Neda Jabbara Golnaz Asaadi tehrani Relationship between Promoter Hypermethylation of DNMT3A and DNMT3B genes and Endometrial Cancer Journal of Epigenetics endometrial cancer promoter hypermethylation msp dnmt3a dnmt3b |
| title | Relationship between Promoter Hypermethylation of DNMT3A and DNMT3B genes and Endometrial Cancer |
| title_full | Relationship between Promoter Hypermethylation of DNMT3A and DNMT3B genes and Endometrial Cancer |
| title_fullStr | Relationship between Promoter Hypermethylation of DNMT3A and DNMT3B genes and Endometrial Cancer |
| title_full_unstemmed | Relationship between Promoter Hypermethylation of DNMT3A and DNMT3B genes and Endometrial Cancer |
| title_short | Relationship between Promoter Hypermethylation of DNMT3A and DNMT3B genes and Endometrial Cancer |
| title_sort | relationship between promoter hypermethylation of dnmt3a and dnmt3b genes and endometrial cancer |
| topic | endometrial cancer promoter hypermethylation msp dnmt3a dnmt3b |
| url | https://jep.usb.ac.ir/article_4476_0fad5f920205862869910d78d675fcfb.pdf |
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