Proteomics as a Quality Control Tool of Pharmaceutical Probiotic Bacterial Lysate Products.
Probiotic bacteria have a wide range of applications in veterinary and human therapeutics. Inactivated probiotics are complex samples and quality control (QC) should measure as many molecular features as possible. Capillary electrophoresis coupled to mass spectrometry (CE/MS) has been used as a mult...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3686750?pdf=render |
| _version_ | 1818952511569002496 |
|---|---|
| author | Günter Klein Joost P Schanstra Janosch Hoffmann Harald Mischak Justyna Siwy Kurt Zimmermann |
| author_facet | Günter Klein Joost P Schanstra Janosch Hoffmann Harald Mischak Justyna Siwy Kurt Zimmermann |
| author_sort | Günter Klein |
| collection | DOAJ |
| description | Probiotic bacteria have a wide range of applications in veterinary and human therapeutics. Inactivated probiotics are complex samples and quality control (QC) should measure as many molecular features as possible. Capillary electrophoresis coupled to mass spectrometry (CE/MS) has been used as a multidimensional and high throughput method for the identification and validation of biomarkers of disease in complex biological samples such as biofluids. In this study we evaluate the suitability of CE/MS to measure the consistency of different lots of the probiotic formulation Pro-Symbioflor which is a bacterial lysate of heat-inactivated Escherichia coli and Enterococcus faecalis. Over 5000 peptides were detected by CE/MS in 5 different lots of the bacterial lysate and in a sample of culture medium. 71 to 75% of the total peptide content was identical in all lots. This percentage increased to 87-89% when allowing the absence of a peptide in one of the 5 samples. These results, based on over 2000 peptides, suggest high similarity of the 5 different lots. Sequence analysis identified peptides of both E. coli and E. faecalis and peptides originating from the culture medium, thus confirming the presence of the strains in the formulation. Ontology analysis suggested that the majority of the peptides identified for E. coli originated from the cell membrane or the fimbrium, while peptides identified for E. faecalis were enriched for peptides originating from the cytoplasm. The bacterial lysate peptides as a whole are recognised as highly conserved molecular patterns by the innate immune system as microbe associated molecular pattern (MAMP). Sequence analysis also identified the presence of soybean, yeast and casein protein fragments that are part of the formulation of the culture medium. In conclusion CE/MS seems an appropriate QC tool to analyze complex biological products such as inactivated probiotic formulations and allows determining the similarity between lots. |
| first_indexed | 2024-12-20T09:51:33Z |
| format | Article |
| id | doaj.art-f68f2e7908d540a3b772a4a90c4563fe |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T09:51:33Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f68f2e7908d540a3b772a4a90c4563fe2022-12-21T19:44:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6668210.1371/journal.pone.0066682Proteomics as a Quality Control Tool of Pharmaceutical Probiotic Bacterial Lysate Products.Günter KleinJoost P SchanstraJanosch HoffmannHarald MischakJustyna SiwyKurt ZimmermannProbiotic bacteria have a wide range of applications in veterinary and human therapeutics. Inactivated probiotics are complex samples and quality control (QC) should measure as many molecular features as possible. Capillary electrophoresis coupled to mass spectrometry (CE/MS) has been used as a multidimensional and high throughput method for the identification and validation of biomarkers of disease in complex biological samples such as biofluids. In this study we evaluate the suitability of CE/MS to measure the consistency of different lots of the probiotic formulation Pro-Symbioflor which is a bacterial lysate of heat-inactivated Escherichia coli and Enterococcus faecalis. Over 5000 peptides were detected by CE/MS in 5 different lots of the bacterial lysate and in a sample of culture medium. 71 to 75% of the total peptide content was identical in all lots. This percentage increased to 87-89% when allowing the absence of a peptide in one of the 5 samples. These results, based on over 2000 peptides, suggest high similarity of the 5 different lots. Sequence analysis identified peptides of both E. coli and E. faecalis and peptides originating from the culture medium, thus confirming the presence of the strains in the formulation. Ontology analysis suggested that the majority of the peptides identified for E. coli originated from the cell membrane or the fimbrium, while peptides identified for E. faecalis were enriched for peptides originating from the cytoplasm. The bacterial lysate peptides as a whole are recognised as highly conserved molecular patterns by the innate immune system as microbe associated molecular pattern (MAMP). Sequence analysis also identified the presence of soybean, yeast and casein protein fragments that are part of the formulation of the culture medium. In conclusion CE/MS seems an appropriate QC tool to analyze complex biological products such as inactivated probiotic formulations and allows determining the similarity between lots.http://europepmc.org/articles/PMC3686750?pdf=render |
| spellingShingle | Günter Klein Joost P Schanstra Janosch Hoffmann Harald Mischak Justyna Siwy Kurt Zimmermann Proteomics as a Quality Control Tool of Pharmaceutical Probiotic Bacterial Lysate Products. PLoS ONE |
| title | Proteomics as a Quality Control Tool of Pharmaceutical Probiotic Bacterial Lysate Products. |
| title_full | Proteomics as a Quality Control Tool of Pharmaceutical Probiotic Bacterial Lysate Products. |
| title_fullStr | Proteomics as a Quality Control Tool of Pharmaceutical Probiotic Bacterial Lysate Products. |
| title_full_unstemmed | Proteomics as a Quality Control Tool of Pharmaceutical Probiotic Bacterial Lysate Products. |
| title_short | Proteomics as a Quality Control Tool of Pharmaceutical Probiotic Bacterial Lysate Products. |
| title_sort | proteomics as a quality control tool of pharmaceutical probiotic bacterial lysate products |
| url | http://europepmc.org/articles/PMC3686750?pdf=render |
| work_keys_str_mv | AT gunterklein proteomicsasaqualitycontroltoolofpharmaceuticalprobioticbacteriallysateproducts AT joostpschanstra proteomicsasaqualitycontroltoolofpharmaceuticalprobioticbacteriallysateproducts AT janoschhoffmann proteomicsasaqualitycontroltoolofpharmaceuticalprobioticbacteriallysateproducts AT haraldmischak proteomicsasaqualitycontroltoolofpharmaceuticalprobioticbacteriallysateproducts AT justynasiwy proteomicsasaqualitycontroltoolofpharmaceuticalprobioticbacteriallysateproducts AT kurtzimmermann proteomicsasaqualitycontroltoolofpharmaceuticalprobioticbacteriallysateproducts |