Effect of quercetin on steroidogenesis and folliculogenesis in ovary of mice with experimentally-induced polycystic ovarian syndrome
IntroductionPolycystic Ovary syndrome (PCOS) affects the health of many women around the world. Apart from fundamental metabolic problems connected to PCOS, focus of our study is on the role of quercetin on genes relevant to steroidogenesis and folliculogenesis.MethodsEighteen mature parkes strain m...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1153289/full |
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| author | Mohd Zahoor ul haq Shah Vinoy kumar Shrivastva Manzoor Ahmad Mir Wajid Mohammad Sheikh Mohd Ashraf Ganie Gulzar Ahmed Rather Majid Shafi Showkeen Muzamil Bashir Mohammad Azam Ansari Meneerah A. Al-Jafary Mohammad H. Al-Qahtani Abdalelgadir Musa Homeida Ebtesam A. Al-Suhaimi |
| author_facet | Mohd Zahoor ul haq Shah Vinoy kumar Shrivastva Manzoor Ahmad Mir Wajid Mohammad Sheikh Mohd Ashraf Ganie Gulzar Ahmed Rather Majid Shafi Showkeen Muzamil Bashir Mohammad Azam Ansari Meneerah A. Al-Jafary Mohammad H. Al-Qahtani Abdalelgadir Musa Homeida Ebtesam A. Al-Suhaimi |
| author_sort | Mohd Zahoor ul haq Shah |
| collection | DOAJ |
| description | IntroductionPolycystic Ovary syndrome (PCOS) affects the health of many women around the world. Apart from fundamental metabolic problems connected to PCOS, focus of our study is on the role of quercetin on genes relevant to steroidogenesis and folliculogenesis.MethodsEighteen mature parkes strain mice (4-5 weeks old) weighing 18–21 g were randomly divided into three groups of six each as follows: Group I serves as the control and was given water and a regular chow diet ad lib for 66 days; group II was given oral gavage administration of letrozole (LETZ) (6 mg/kg bw) for 21 days to induce PCOS and was left untreated for 45 days; For three weeks, Group III received oral gavage dose of LETZ (6 mg/kg), after which it received Quercetin (QUER) (125 mg/kg bw orally daily) for 45 days.ResultsIn our study we observed that mice with PCOS had irregular estrous cycle with increased LH/FSH ratio, decreased estrogen level and decline in expression of Kitl, Bmp1, Cyp11a1, Cyp19a1, Ar, lhr, Fshr and Esr1 in ovary. Moreover, we observed increase in the expression of CYP17a1, as well as increase in cholesterol, triglycerides, testosterone, vascular endothelial growth factor VEGF and insulin levels. All these changes were reversed after the administration of quercetin in PCOS mice.DiscussionQuercetin treatment reversed the molecular, functional and morphological abnormalities brought on due to letrozole in pathological and physiological setting, particularly the issues of reproduction connected to PCOS. Quercetin doesn’t act locally only but it acts systematically as it works on Pituitary (LH/FSH)- Ovary (gonad hormones) axis. the Side effects of Quercetin have to be targeted in future researches. Quercetin may act as a promising candidate for medical management of human PCOS. |
| first_indexed | 2024-03-12T14:04:21Z |
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| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-03-12T14:04:21Z |
| publishDate | 2023-04-01 |
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| spelling | doaj.art-f692bae977344c5bbf10c19765d28be32023-08-21T15:44:57ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-04-011410.3389/fendo.2023.11532891153289Effect of quercetin on steroidogenesis and folliculogenesis in ovary of mice with experimentally-induced polycystic ovarian syndromeMohd Zahoor ul haq Shah0Vinoy kumar Shrivastva1Manzoor Ahmad Mir2Wajid Mohammad Sheikh3Mohd Ashraf Ganie4Gulzar Ahmed Rather5Majid Shafi6Showkeen Muzamil Bashir7Mohammad Azam Ansari8Meneerah A. Al-Jafary9Mohammad H. Al-Qahtani10Abdalelgadir Musa Homeida11Ebtesam A. Al-Suhaimi12Laboratory of Endocrinology, Department of Bioscience, Barkatullah University, Bhopal, Madhya Pradesh, IndiaLaboratory of Endocrinology, Department of Bioscience, Barkatullah University, Bhopal, Madhya Pradesh, IndiaDepartment of Bioresources, University of Kashmir, Srinagar, IndiaBiochemistry & Molecular Biology Lab, Division of Veterinary Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, IndiaDepartment of Endocrinology and Metabolism, Sher-i-Kashmir Institute of Medical Sciences, Srinagar, IndiaDepartment of Biomedical Engineering, Sathyabama Institute of Science & Technology, Chennai, Tamil Nadu, IndiaDivision of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, IndiaBiochemistry & Molecular Biology Lab, Division of Veterinary Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, IndiaDepartment of Epidemic Disease Research, Institute for Research & Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi ArabiaBiology Department, College of Science and Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi ArabiaDepartment of Paediatrics, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia0Department of Environmental Health Research, Institute for Research & Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi ArabiaBiology Department, College of Science and Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi ArabiaIntroductionPolycystic Ovary syndrome (PCOS) affects the health of many women around the world. Apart from fundamental metabolic problems connected to PCOS, focus of our study is on the role of quercetin on genes relevant to steroidogenesis and folliculogenesis.MethodsEighteen mature parkes strain mice (4-5 weeks old) weighing 18–21 g were randomly divided into three groups of six each as follows: Group I serves as the control and was given water and a regular chow diet ad lib for 66 days; group II was given oral gavage administration of letrozole (LETZ) (6 mg/kg bw) for 21 days to induce PCOS and was left untreated for 45 days; For three weeks, Group III received oral gavage dose of LETZ (6 mg/kg), after which it received Quercetin (QUER) (125 mg/kg bw orally daily) for 45 days.ResultsIn our study we observed that mice with PCOS had irregular estrous cycle with increased LH/FSH ratio, decreased estrogen level and decline in expression of Kitl, Bmp1, Cyp11a1, Cyp19a1, Ar, lhr, Fshr and Esr1 in ovary. Moreover, we observed increase in the expression of CYP17a1, as well as increase in cholesterol, triglycerides, testosterone, vascular endothelial growth factor VEGF and insulin levels. All these changes were reversed after the administration of quercetin in PCOS mice.DiscussionQuercetin treatment reversed the molecular, functional and morphological abnormalities brought on due to letrozole in pathological and physiological setting, particularly the issues of reproduction connected to PCOS. Quercetin doesn’t act locally only but it acts systematically as it works on Pituitary (LH/FSH)- Ovary (gonad hormones) axis. the Side effects of Quercetin have to be targeted in future researches. Quercetin may act as a promising candidate for medical management of human PCOS.https://www.frontiersin.org/articles/10.3389/fendo.2023.1153289/fullPCOS (polycystic ovarian syndrome)steroidogenesisfolliculogenesisquercetinVEGF |
| spellingShingle | Mohd Zahoor ul haq Shah Vinoy kumar Shrivastva Manzoor Ahmad Mir Wajid Mohammad Sheikh Mohd Ashraf Ganie Gulzar Ahmed Rather Majid Shafi Showkeen Muzamil Bashir Mohammad Azam Ansari Meneerah A. Al-Jafary Mohammad H. Al-Qahtani Abdalelgadir Musa Homeida Ebtesam A. Al-Suhaimi Effect of quercetin on steroidogenesis and folliculogenesis in ovary of mice with experimentally-induced polycystic ovarian syndrome Frontiers in Endocrinology PCOS (polycystic ovarian syndrome) steroidogenesis folliculogenesis quercetin VEGF |
| title | Effect of quercetin on steroidogenesis and folliculogenesis in ovary of mice with experimentally-induced polycystic ovarian syndrome |
| title_full | Effect of quercetin on steroidogenesis and folliculogenesis in ovary of mice with experimentally-induced polycystic ovarian syndrome |
| title_fullStr | Effect of quercetin on steroidogenesis and folliculogenesis in ovary of mice with experimentally-induced polycystic ovarian syndrome |
| title_full_unstemmed | Effect of quercetin on steroidogenesis and folliculogenesis in ovary of mice with experimentally-induced polycystic ovarian syndrome |
| title_short | Effect of quercetin on steroidogenesis and folliculogenesis in ovary of mice with experimentally-induced polycystic ovarian syndrome |
| title_sort | effect of quercetin on steroidogenesis and folliculogenesis in ovary of mice with experimentally induced polycystic ovarian syndrome |
| topic | PCOS (polycystic ovarian syndrome) steroidogenesis folliculogenesis quercetin VEGF |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1153289/full |
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