Optimization of the Extraction Process of Anthocyanins from Perilla Leaves and Their Antioxidant and Antibacterial Activities
In order to determine the optimal extraction process, and antioxidant and antibacterial activities of anthocyanins from Perilla friesians leaf, in this study, Perilla friesians leaf was used as raw material. Response surface method was used to optimize the optimal process conditions of ultrasonic-as...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-10-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022100307 |
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| author | Jiangyi SUN Yongyi LONG Shiqi LIU Li ZHANG Qinqin MA Bingyao BAI Lijun SONG |
| author_facet | Jiangyi SUN Yongyi LONG Shiqi LIU Li ZHANG Qinqin MA Bingyao BAI Lijun SONG |
| author_sort | Jiangyi SUN |
| collection | DOAJ |
| description | In order to determine the optimal extraction process, and antioxidant and antibacterial activities of anthocyanins from Perilla friesians leaf, in this study, Perilla friesians leaf was used as raw material. Response surface method was used to optimize the optimal process conditions of ultrasonic-assisted extraction of anthocyanins from Perilla friesians leaf, and liquid mass combined technology was used to conduct qualitative and quantitative analysis of anthocyanins and anthocyanins in the extracts. The antioxidant activity and antibacterial activity of the extracts were studied. The results showed that the optimum conditions were as follows: The ratio of material to liquid was 1:20 g/mL, the ultrasonic temperature was 47 ℃, the ultrasonic time was 57 min, and ethanol concentration was 62%, and the maximum extraction rate was 7.18±0.32 mg/g DW under these conditions. The extract was mainly composed of cyanidin, delphinidin 3-O-glucoside, and cyanidin 3-O-glucoside. The extract showed significant scavenging activity on DPPH and ABTS+ free radicals, and the IC50 values were 0.49 and 0.32 mg/mL, respectively. The lowest inhibitory concentration against Staphylococcus aureus was 160 mg/mL. This study provides a theoretical basis for the extraction and application of anthocyanins from Perilla friesians leaf. |
| first_indexed | 2024-03-11T18:52:11Z |
| format | Article |
| id | doaj.art-f6930dbec72b4493bd7551910d58aa6f |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-11T18:52:11Z |
| publishDate | 2023-10-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-f6930dbec72b4493bd7551910d58aa6f2023-10-11T09:18:20ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-10-01441919119810.13386/j.issn1002-0306.20221003072022100307-19Optimization of the Extraction Process of Anthocyanins from Perilla Leaves and Their Antioxidant and Antibacterial ActivitiesJiangyi SUN0Yongyi LONG1Shiqi LIU2Li ZHANG3Qinqin MA4Bingyao BAI5Lijun SONG6School of Food Science and Engineering, Tarim University, Alar 843300, ChinaSchool of Food Science and Engineering, Tarim University, Alar 843300, ChinaCollege of Food Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066600, ChinaCollege of Food Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066600, ChinaCollege of Life Sciences, Sichuan Normal University, Chengdu 610066, ChinaSchool of Food Science and Engineering, Tarim University, Alar 843300, ChinaCollege of Food Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066600, ChinaIn order to determine the optimal extraction process, and antioxidant and antibacterial activities of anthocyanins from Perilla friesians leaf, in this study, Perilla friesians leaf was used as raw material. Response surface method was used to optimize the optimal process conditions of ultrasonic-assisted extraction of anthocyanins from Perilla friesians leaf, and liquid mass combined technology was used to conduct qualitative and quantitative analysis of anthocyanins and anthocyanins in the extracts. The antioxidant activity and antibacterial activity of the extracts were studied. The results showed that the optimum conditions were as follows: The ratio of material to liquid was 1:20 g/mL, the ultrasonic temperature was 47 ℃, the ultrasonic time was 57 min, and ethanol concentration was 62%, and the maximum extraction rate was 7.18±0.32 mg/g DW under these conditions. The extract was mainly composed of cyanidin, delphinidin 3-O-glucoside, and cyanidin 3-O-glucoside. The extract showed significant scavenging activity on DPPH and ABTS+ free radicals, and the IC50 values were 0.49 and 0.32 mg/mL, respectively. The lowest inhibitory concentration against Staphylococcus aureus was 160 mg/mL. This study provides a theoretical basis for the extraction and application of anthocyanins from Perilla friesians leaf.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022100307perilla leafanthocyaninsresponse surface methodantioxidant activityantibacterial activity |
| spellingShingle | Jiangyi SUN Yongyi LONG Shiqi LIU Li ZHANG Qinqin MA Bingyao BAI Lijun SONG Optimization of the Extraction Process of Anthocyanins from Perilla Leaves and Their Antioxidant and Antibacterial Activities Shipin gongye ke-ji perilla leaf anthocyanins response surface method antioxidant activity antibacterial activity |
| title | Optimization of the Extraction Process of Anthocyanins from Perilla Leaves and Their Antioxidant and Antibacterial Activities |
| title_full | Optimization of the Extraction Process of Anthocyanins from Perilla Leaves and Their Antioxidant and Antibacterial Activities |
| title_fullStr | Optimization of the Extraction Process of Anthocyanins from Perilla Leaves and Their Antioxidant and Antibacterial Activities |
| title_full_unstemmed | Optimization of the Extraction Process of Anthocyanins from Perilla Leaves and Their Antioxidant and Antibacterial Activities |
| title_short | Optimization of the Extraction Process of Anthocyanins from Perilla Leaves and Their Antioxidant and Antibacterial Activities |
| title_sort | optimization of the extraction process of anthocyanins from perilla leaves and their antioxidant and antibacterial activities |
| topic | perilla leaf anthocyanins response surface method antioxidant activity antibacterial activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022100307 |
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