In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa
An extensive protein-interaction network of cell-wall biosynthesis assemblies in P. aeruginosa is elucidated using a pulldown strategy with mass-spectrometry-based proteomics and binding assays.
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
|
| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-022-04230-x |
| _version_ | 1797985452236472320 |
|---|---|
| author | Luis F. Avila-Cobian Stefania De Benedetti Choon Kim Rhona Feltzer Matthew M. Champion Jed F. Fisher Shahriar Mobashery |
| author_facet | Luis F. Avila-Cobian Stefania De Benedetti Choon Kim Rhona Feltzer Matthew M. Champion Jed F. Fisher Shahriar Mobashery |
| author_sort | Luis F. Avila-Cobian |
| collection | DOAJ |
| description | An extensive protein-interaction network of cell-wall biosynthesis assemblies in P. aeruginosa is elucidated using a pulldown strategy with mass-spectrometry-based proteomics and binding assays. |
| first_indexed | 2024-04-11T07:18:21Z |
| format | Article |
| id | doaj.art-f69676158d564c2ba1d77745bd267f7a |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-04-11T07:18:21Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-f69676158d564c2ba1d77745bd267f7a2022-12-22T04:37:52ZengNature PortfolioCommunications Biology2399-36422022-11-015111610.1038/s42003-022-04230-xIn vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosaLuis F. Avila-Cobian0Stefania De Benedetti1Choon Kim2Rhona Feltzer3Matthew M. Champion4Jed F. Fisher5Shahriar Mobashery6Department of Chemistry and Biochemistry, University of Notre DameDepartment of Chemistry and Biochemistry, University of Notre DameDepartment of Chemistry and Biochemistry, University of Notre DameDepartment of Chemistry and Biochemistry, University of Notre DameDepartment of Chemistry and Biochemistry, University of Notre DameDepartment of Chemistry and Biochemistry, University of Notre DameDepartment of Chemistry and Biochemistry, University of Notre DameAn extensive protein-interaction network of cell-wall biosynthesis assemblies in P. aeruginosa is elucidated using a pulldown strategy with mass-spectrometry-based proteomics and binding assays.https://doi.org/10.1038/s42003-022-04230-x |
| spellingShingle | Luis F. Avila-Cobian Stefania De Benedetti Choon Kim Rhona Feltzer Matthew M. Champion Jed F. Fisher Shahriar Mobashery In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa Communications Biology |
| title | In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa |
| title_full | In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa |
| title_fullStr | In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa |
| title_full_unstemmed | In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa |
| title_short | In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa |
| title_sort | in vitro studies of the protein interaction network of cell wall lytic transglycosylase rlpa of pseudomonas aeruginosa |
| url | https://doi.org/10.1038/s42003-022-04230-x |
| work_keys_str_mv | AT luisfavilacobian invitrostudiesoftheproteininteractionnetworkofcellwalllytictransglycosylaserlpaofpseudomonasaeruginosa AT stefaniadebenedetti invitrostudiesoftheproteininteractionnetworkofcellwalllytictransglycosylaserlpaofpseudomonasaeruginosa AT choonkim invitrostudiesoftheproteininteractionnetworkofcellwalllytictransglycosylaserlpaofpseudomonasaeruginosa AT rhonafeltzer invitrostudiesoftheproteininteractionnetworkofcellwalllytictransglycosylaserlpaofpseudomonasaeruginosa AT matthewmchampion invitrostudiesoftheproteininteractionnetworkofcellwalllytictransglycosylaserlpaofpseudomonasaeruginosa AT jedffisher invitrostudiesoftheproteininteractionnetworkofcellwalllytictransglycosylaserlpaofpseudomonasaeruginosa AT shahriarmobashery invitrostudiesoftheproteininteractionnetworkofcellwalllytictransglycosylaserlpaofpseudomonasaeruginosa |